Background: Alveolar bone related defect has emerged as a major challenge for clinician. Previous studies reported that concentrated growth factors (CGF), an autogenous product derived from venous blood, could enhance healing of grafts via releasing various growth factors. Methods: This study was designed to investigate the osteogenesis of CGF gel accompanied with Bio-Oss in canine alveolar defect socket model. CGF gel were prepared via variable speed centrifugation. Immunohistochemical staining and semi-quantitative analysis were performed to evaluate the content of transforming growth factor-β1 (TGF-β1) and vascular endothelial growth factor (VEGF) in the CGF gel and red blood cell (RBC) layer. The decay rate of TGF-β1 and VEGF were determined by ELISA assay. Next, mixture of Bio-Oss and CGF gel was implanted as graft to the alveolar defect socket. Three different fluorescent dyes and toluidine blue staining were utilized to track osteogenic progress, and mineral apposition rate (MAR) was calculated. Results: In the CGF gel, TGF-β1 occupied the dominant content with a higher decay rate than that of VEGF. Whereas, the concentration of TGF-β1 released from CGF gel still raised up rapidly within 14 days. In the animal study, combination of Bio-Oss and CGF gel not only accelerated wound healing, but also succussed to activate and sustain the bone formation at defect site represented as a higher MAR (2.21 ± 0.52 µm/day vs 1.43 ± 0.41 µm/day, Bio-Oss group and 1.04 ± 0.26 µm/day, control groups, P < 0.05). Conclusions: combination of Bio-Oss and CGF gel could promote osteogenesis and might provide a promising strategy against alveolar bone defect.