A convenient and reproducible method to genetically transform bacteria of the genus Bifidobacterium

Argnani, A; Leer, R.J.; Luijk, N. van; Pouwels, Peter H.

doi:10.1099/13500872-142-1-109

Cited by 64 publications

(44 citation statements)

References 26 publications

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“…3). It is also noteworthy that Corynebacterium replicons, known to replicate in bifidobacteria (Argnani et al 1996;Schürch 2002), contain replication proteins similar to typologies II, III, and IV (Fig. 3), suggesting that these replicons may have begun replicating in members of Bifidobacterium and Corynebacterium before the two evolved into distinct genera.…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization of Bifidobacterium longum biovar longum NAL8 plasmids and construction of a novel replicon screening system

Guglielmetti

Karp

Mora

et al. 2007

Appl Microbiol Biotechnol

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In this study, we performed molecular characterization and sequence analysis of three plasmids from the human intestinal isolate Bifidobacterium longum biovar longum NAL8 and developed a novel vector screening system. Plasmids pNAL8H (10 kb) and pNAL8M (4.9 kb) show close sequence similarity to and the same gene organization as the already characterized B. longum plasmids. The B. longum plasmid pNAC1 was identified as being most closely related to pNAL8L (3.5 kb). However, DNA sequence analysis suggested that direct repeat-rich sites could have promoted several recombination events to diversify the two plasmid molecules. We verified the likely rolling circle replication of plasmid pNAL8L and studied the phylogenetic relationship in all the Bifidobacterium plasmids fully sequenced to date based on in silico comparative sequence analysis of their replication proteins and iteron regions. Our transformation experiments confirmed that the ColE1 replication origin from high-copy-number pUC vectors could interfere with the replication apparatus of Bifidobacterium plasmids and give rise to false positive clones. As a result, we developed a system suitable for avoiding possible interference by other functional replication modules on the vector and for screening functional replicons from wildtype plasmids.

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Section: Discussionmentioning

confidence: 99%

Molecular characterization of Bifidobacterium longum biovar longum NAL8 plasmids and construction of a novel replicon screening system

Guglielmetti

Karp

Mora

et al. 2007

Appl Microbiol Biotechnol

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“…Plasmid construction and transformation of B. adolescentis 12 A shuttle vector, pBV220, 13 containing Amp r gene, was from the Institute of Virology, Chinese Academy of Preventive Medicine. Human endostatin gene was obtained by PCR method from human liver cDNA library ( Clontech, Franklin Lakes, NJ ) and inserted into pBV220 vector.…”

Section: B Adolescentismentioning

confidence: 99%

Bifidobacterium adolescentis as a delivery system of endostatin for cancer gene therapy: Selective inhibitor of angiogenesis and hypoxic tumor growth

Fan

et al. 2003

Cancer Gene Ther

137

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In order to overcome difficulties that hampered widespread application of antiangiogenesis in cancer therapy, a highly specific delivery system may be engaged in vivo to deliver and express antiangiogenic genes. We selected a strain of Bifidobacterium adolescentis ( B. adolescentis ) as the delivery system to transport endostatin gene to solid tumors. B. adolescentis with endostatin gene were injected into tumor -bearing mice through the tail vein. After the mice were sacrificed, the tumor and some normal tissues of the mice were examined. B. adolescentis were only found in the tumors and no bacilli were found in other normal tissues. Also, a strong inhibition of angiogenesis had been shown to inhibit local tumor growth in the administrated group. These results suggested that B. adolescentis only germinated and proliferated in solid tumors and might be a highly specific and efficient vector for transporting anticancer genes into target tumor in cancer gene therapy.

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“…shuttle vector pBLES 1 00; transformation by electroporation; spectinomycin adenyl transferase AAD (9) Bifidobacteria are Gram-positive anaerobic bacteria, the genome of which is GC-rich.l) These microorganisms constitute a major part of the normal microflora in the large intestine of humans and some other animals.…”

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confidence: 99%

Construction ofEscherichia coli–Bifidobacterium longumShuttle Vector TransformingB. longum105-A and 108-A

Matsumura

Takeuchi

Kano

1997

Bioscience, Biotechnology, and Biochemistry

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A convenient and reproducible method to genetically transform bacteria of the genus Bifidobacterium

Cited by 64 publications

References 26 publications

Molecular characterization of Bifidobacterium longum biovar longum NAL8 plasmids and construction of a novel replicon screening system

Molecular characterization of Bifidobacterium longum biovar longum NAL8 plasmids and construction of a novel replicon screening system

Bifidobacterium adolescentis as a delivery system of endostatin for cancer gene therapy: Selective inhibitor of angiogenesis and hypoxic tumor growth

Construction ofEscherichia coli–Bifidobacterium longumShuttle Vector TransformingB. longum105-A and 108-A

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