A convenient and versatile hydroponic cultivation system for <i>Arabidopsis thaliana</i>

Norén, Hanna; Svensson, Per H.; Andersson, Bertil

doi:10.1111/j.0031-9317.2004.00350.x

Cited by 75 publications

(69 citation statements)

References 20 publications

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“…Fig. 5B 19 and 23% of the assayed uptake of ATP and ADP, respectively. Furthermore, radioactive nucleotides were detected in washed and disrupted expressing cells (see Fig.…”

Section: Resultsmentioning

confidence: 94%

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Identification, Expression, and Functional Analyses of a Thylakoid ATP/ADP Carrier from Arabidopsis

Thuswaldner

Lagerstedt²,

Rojas-Stütz

et al. 2007

Journal of Biological Chemistry

106

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TAAC is readily expressed in dark-grown Arabidopsis seedlings, and its level remains stable throughout the greening process. Its expression is highest in developing green tissues and in leaves undergoing senescence or abiotic stress. We propose that the TAAC protein supplies ATP for energy-dependent reactions during thylakoid biogenesis and turnover in plants.Chloroplasts perform oxygenic photosynthesis in algae and plants and have evolved by endosymbiosis from cyanobacteria. Chloroplasts have two distinct membrane systems, the double envelope surrounding the organelle and an internal membrane system named thylakoids. The envelope membrane represents the interface between the cytoplasm and chloroplast stroma, whereas the thylakoid membrane separates the stroma and the lumenal space. Altogether ϳ800 membrane proteins have been identified by proteomics in the envelope and thylakoid membranes of Arabidopsis thaliana (for reviews, see Refs. 1 and 2). As expected, the main function for the identified envelope proteins was transport of ions and metabolites, whereas photosynthesis was attributed to most of the identified thylakoid proteins. The major protein complexes in thylakoids are photosystems (PS) 4 I and II, the cytochrome b 6 f complex, and the proton-translocating ATP synthase. These photosynthetic complexes contain not only proteins but also pigments and other cofactors. Their assembly, activity, and removal require a large number of auxiliary, regulatory, and transport proteins (3, 4). Many biochemical reports pointed to the existence of transport activities in the thylakoid membrane, such as calcium transport (5), copper transport (6), anion channels (7), cation channels (8, 9), and nucleotide transport (10). Only the thylakoid copper transporter was identified at the genetic level in Arabidopsis (11). No hydrophobic proteins related to the above-mentioned transport activities were identified in the previous proteomic works on Arabidopsis thylakoid membranes (for a review, see Ref.2). Therefore, genetic strategies are required for identification and elucidation of their role in optimal function of the thylakoid.ATP is produced during the light-dependent photosynthetic reactions on the stromal side of the thylakoid membrane. Besides its utilization during CO 2 fixation in the stroma, ATP drives many energy-dependent processes in thylakoids, including protein phosphorylation, folding, import, and degradation.

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“…Fig. 5B 19 and 23% of the assayed uptake of ATP and ADP, respectively. Furthermore, radioactive nucleotides were detected in washed and disrupted expressing cells (see Fig.…”

Section: Resultsmentioning

confidence: 94%

“…Columbia) plants were grown hydroponically (19) Arabidopsis (cv. Wassilewskaja) seeds of the T-DNA insertion line FLAG 443D03 (taac mutant, supplemental Fig.…”

Section: Methodsmentioning

confidence: 99%

Identification, Expression, and Functional Analyses of a Thylakoid ATP/ADP Carrier from Arabidopsis

Thuswaldner

Lagerstedt²,

Rojas-Stütz

et al. 2007

Journal of Biological Chemistry

106

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“…Arabidopsis thaliana wild-type (ecotype Columbia-0) plants and three kinase mutants, stn7 (SALK 073254 [2]), stn8 (SALK 060869 [3]) and the double mutant stn7stn8 [7] were grown hydroponically [18] 0 -GCT TCT TCC TCT GCT CTT TCC for SALK 057443c) and a T-DNA specific primer (5 0 -TGG TTC ACG TAG TGG GCC ATC G-3 0 ). The absence of pTAC16 at the protein level was verified by immunoblotting using the pTAC16-specific antibody.…”

Section: Plant Materialsmentioning

confidence: 99%

Phosphoproteomics of Arabidopsis chloroplasts reveals involvement of the STN7 kinase in phosphorylation of nucleoid protein pTAC16

Ingelsson

Vener

2012

FEBS Letters

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a b s t r a c tLight-regulated protein kinases STN7 and STN8 phosphorylate thylakoid membrane proteins and also affect expression of several chloroplast proteins via yet unknown mechanisms. Comparative phosphoproteomics of acetic acid protein extracts of chloroplasts from Arabidopsis thaliana wild type, stn7, stn8 and stn7stn8 mutants yielded two previously unknown findings: (i) neither STN7 nor STN8 kinase was required for phosphorylation of Ser-48 in Lhcb1.1-1.3 proteins; and (ii) phosphorylation of Thr-451 in pTAC16 protein was STN7-dependent. pTAC16 was found distributed between thylakoids and nucleoid. Its knockout did not affect the nucleoid protein composition and the Thr-451 phosphorylated protein was excluded from the nucleoid. Thr-451 of pTAC16 is conserved in all studied plants and its phosphorylation may regulate membrane-anchoring functions of the nucleoid. Structured summary with protein interactions:RPOC2, RPOB, pTAC3, emb2746, RPOC1, pTAC2, pTAC12, pTAC16, pTAC5, pTAC14, RPOA, pTAC13, pTAC15 and pTAC6 colocalize by mass spectrometry studies of complexes (View interaction) PsbH, Lhcb4,1, Lhcb4,2, STN7, D1, D2, CP43, TSP9, CaS, PsaP, Alb3, PsbL, Rubisco, Unknown, Unknown, OEP6, pTAC16, PGRL1A, Unknown, Unknown and PsbQ colocalize by mass spectrometry studies of complexes (View interaction)

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“…Plants were grown in chambers at 21°C with 70% relative humidity and an 8-h photoperiod (150 mmol m 22 s 21 ) in soil (SunGro Redi-earth) or hydroponically as described previously (Noren et al, 2004;Guo et al, 2008b).…”

Section: Plant Materials and Growth Conditionsmentioning

confidence: 99%

The Sink-Specific Plastidic Phosphate Transporter PHT4;2 Influences Starch Accumulation and Leaf Size in Arabidopsis

et al. 2011

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Nonphotosynthetic plastids are important sites for the biosynthesis of starch, fatty acids, and amino acids. The uptake and subsequent use of cytosolic ATP to fuel these and other anabolic processes would lead to the accumulation of inorganic phosphate (Pi) if not balanced by a Pi export activity. However, the identity of the transporter(s) responsible for Pi export is unclear. The plastid-localized Pi transporter PHT4;2 of Arabidopsis (Arabidopsis thaliana) is expressed in multiple sink organs but is nearly restricted to roots during vegetative growth. We identified and used pht4;2 null mutants to confirm that PHT4;2 contributes to Pi transport in isolated root plastids. Starch accumulation was limited in pht4;2 roots, which is consistent with the inhibition of starch synthesis by excess Pi as a result of a defect in Pi export. Reduced starch accumulation in leaves and altered expression patterns for starch synthesis genes and other plastid transporter genes suggest metabolic adaptation to the defect in roots. Moreover, pht4;2 rosettes, but not roots, were significantly larger than those of the wild type, with 40% greater leaf area and twice the biomass when plants were grown with a short (8-h) photoperiod. Increased cell proliferation accounted for the larger leaf size and biomass, as no changes were detected in mature cell size, specific leaf area, or relative photosynthetic electron transport activity. These data suggest novel signaling between roots and leaves that contributes to the regulation of leaf size.

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A convenient and versatile hydroponic cultivation system for Arabidopsis thaliana

Cited by 75 publications

References 20 publications

Identification, Expression, and Functional Analyses of a Thylakoid ATP/ADP Carrier from Arabidopsis

Identification, Expression, and Functional Analyses of a Thylakoid ATP/ADP Carrier from Arabidopsis

Phosphoproteomics of Arabidopsis chloroplasts reveals involvement of the STN7 kinase in phosphorylation of nucleoid protein pTAC16

The Sink-Specific Plastidic Phosphate Transporter PHT4;2 Influences Starch Accumulation and Leaf Size in Arabidopsis

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