1992
DOI: 10.1021/ja00029a004
|View full text |Cite
|
Sign up to set email alerts
|

A convergent approach to protein design. Metal ion-assisted spontaneous self-assembly of a polypeptide into a triple-helix bundle protein

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1

Citation Types

2
140
1
1

Year Published

1998
1998
2005
2005

Publication Types

Select...
7
1

Relationship

0
8

Authors

Journals

citations
Cited by 235 publications
(144 citation statements)
references
References 0 publications
2
140
1
1
Order By: Relevance
“…NOEs were taken from a NOESY spectrum recorded with a mixing time of 100 ms. The three-bond 2J ␣NH coupling constants were taken from a high-resolution double-quantumfiltered COSY experiment and converted to -angle restraints by using the standard methods (36,37). Only one of the helices is shown for clarity because the other helix gives the same pattern.…”
Section: Resultsmentioning
confidence: 99%
See 1 more Smart Citation
“…NOEs were taken from a NOESY spectrum recorded with a mixing time of 100 ms. The three-bond 2J ␣NH coupling constants were taken from a high-resolution double-quantumfiltered COSY experiment and converted to -angle restraints by using the standard methods (36,37). Only one of the helices is shown for clarity because the other helix gives the same pattern.…”
Section: Resultsmentioning
confidence: 99%
“…Analyses of thermal unfolding data were done with established methods (35). The chemical unfolding data were best-fit by using the nonlinear least-squares algorithms in SIGMA-PLOT to a two-state denaturation function (36). NMR Spectroscopy.…”
mentioning
confidence: 99%
“…More recently, Ghadiri and co-workers have synthesized a peptide containing a 2,2'-bipyridine at the N-terminus 137 . The peptide units were organized through a similar strategy and the proprieties of the systems obtained were studied.…”
Section: Metallo-exoreceptors Formed By Template Strategiesmentioning
confidence: 99%
“…The peptide chain, comprised by a sequence of five different amino acids, possesses a well-established helical structure. Although this peptide may exist in solution as an equilibrium involving the monomer and multimeric aggregates, the addition of Ni 2+ or Co 2+ limits the aggregation to the three-helix bundle, because of the appended bipyridine 137 . The formation of the three-helix structure was confirmed by circular dichroism and mass spectrometry.…”
Section: Metallo-exoreceptors Formed By Template Strategiesmentioning
confidence: 99%
“…The average length over which a helix handedness keeps its integrity is determined by a combination of local conformational preferences in the helix backbone as well as intramolecular and solvent-driven interactions that define the helix propensity associated with the monomers (1)(2)(3)(4)(5)(6)(7). Long-range intermolecular helix-helix interactions and helix bundling have also been shown contribute to helix stability (9)(10)(11)(12)(13). The balance between these factors is subtle, and helix propensity is difficult to predict and characterize.…”
mentioning
confidence: 99%