A Coplanar-Waveguide System for Cells Exposure During Electrophysiological Recordings

Liberti, Micaela; Apollonio, Francesca; Paffi, Alessandra; Pellegrino, M.; D’Inzeo, G.

doi:10.1109/tmtt.2004.837155

Cited by 27 publications

(14 citation statements)

References 11 publications

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“…The exposure system, especially designed for electrophysiological patch-clamp recording, has been described elsewhere [Liberti et al, 2004]. Briefly, it is an open propagating structure consisting of a Co-Planar Wave-guide (CPW) (22.0 cm Â 10.2 cm Â 0.4 cm).…”

Section: Cell Exposure To Rfmentioning

confidence: 99%

“…In this way it is possible to minimize the interference of the field with the electrode (i.e., with the current being measured). Moreover, measurements conducted in scattering parameters showed a low interaction of the system with the laboratory equipment, for example, the microscopic objective [Liberti et al, 2004]. Actually, even though the microscope objective may induce a local perturbation of the field below the substrate, it minimally influences the overall behavior of the exposure system.…”

Section: Cell Exposure To Rfmentioning

confidence: 99%

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Acute exposure to low‐level CW and GSM‐modulated 900 MHz radiofrequency does not affect Ba²⁺ currents through voltage‐gated calcium channels in rat cortical neurons

Platano

Mesirca

Paffi

et al. 2007

Bioelectromagnetics

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We have studied the non-thermal effects of radiofrequency (RF) electromagnetic fields (EMFs) on Ba(2+) currents (I Ba 2+) through voltage-gated calcium channels (VGCC), recorded in primary cultures of rat cortical neurons using the patch-clamp technique. To assess whether low-level acute RF field exposure could modify the amplitude and/or the voltage-dependence of I Ba 2+, Petri dishes containing cultured neurons were exposed for 1-3 periods of 90 s to 900 MHz RF-EMF continuous wave (CW) or amplitude-modulated according to global system mobile communication standard (GSM) during whole-cell recording. The specific absorption rates (SARs) were 2 W/kg for CW and 2 W/kg (time average value) for GSM-modulated signals, respectively. The results obtained indicate that single or multiple acute exposures to either CW or GSM-modulated 900 MHz RF-EMFs do not significantly alter the current amplitude or the current-voltage relationship of I Ba 2+, through VGCC.

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Section: Cell Exposure To Rfmentioning

confidence: 99%

Section: Cell Exposure To Rfmentioning

confidence: 99%

Acute exposure to low‐level CW and GSM‐modulated 900 MHz radiofrequency does not affect Ba²⁺ currents through voltage‐gated calcium channels in rat cortical neurons

Platano

Mesirca

Paffi

et al. 2007

Bioelectromagnetics

View full text Add to dashboard Cite

show abstract

“…Moreover, it should provide an error estimation in order to minimize uncertainty and guarantee the correctness of the solution for each model. Several numerical methods have been employed for Petri dish simulations exposed to EM fields in different configurations, e.g., [3,20,21,16,1,22,23]. Approaches based on differential formulations are mainly used because of their flexibility to deal with complex geometrical and material configurations.…”

Section: Introductionmentioning

confidence: 99%

High-accuracy adaptive modeling of the energy distribution of a meniscus-shaped cell culture in a Petri dish

Gomez-Revuelto

Garcı́a-Castillo

Pardo

2015

Journal of Computational Science

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Cylindrical Petri dishes embedded in a rectangular waveguide and exposed to a polarized electromagnetic wave are often used to grow cell cultures. To guarantee the success of these cultures, it is necessary to enforce that the specific absorption rate distribution is sufficiently high and uniform over the Petri dish. Accurate numerical simulations are needed to design such systems. These simulations constitute a challenge due to the strong discontinuity of electromagnetic material properties involved, the relative low field value within the dish cultures compared with the rest of the domain, and the presence of the meniscus shape developed at the liquid boundary. The latter greatly increases the level of complexity of the model in terms of geometry and intensity of the gradients/singularities of the field solution. In here, we employ a three-dimensional (3D) hp-adaptive finite element method using isoparametric elements to obtain highly accurate simulations. We analyze the impact of the geometrical modeling of the meniscus shape cell culture in the hp-adaptivity. Numerical results showing the error convergence history indicate the numerical difficulties arisen due to the presence of a meniscus-shaped object. At the same time, the resulting energy distribution shows that to consider such meniscus shape is essential to guarantee the success of the cell culture from the biological point of view.

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“…For real-time investigation, specific exposure systems have also been designed incorporating multiple electrode arrays (MEA) [7][8][9] and coplanar waveguides [10,11]. A few studies include monitoring of the sample using microscopic imaging.…”

Section: Introductionmentioning

confidence: 99%

Electromagnetic Analysis of an Aperture Modified Tem Cell Including an Ito Layer for Real-Time Observation of Biological Cells Exposed to Microwaves

Soueid¹,

Kohler²,

Carr³

et al. 2014

PIER

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Abstract-We propose to analyze the aperture and ITO layer presence of a modified transverse electromagnetic (TEM) cell. This TEM cell can be used to study the potential effects of microwave electromagnetic fields on biological cells. This modified delivery device allows real-time observation of biological cells during exposure. Microscopic observation is achieved through an aperture in the lower wall of the TEM cell that is sealed with a 700-nm film of the transparent conducting material Indium tin oxide (ITO). To determine the device efficiency, numerical and experimental electromagnetic dosimetry was conducted. For assessing the effect of the aperture on the specific absorption rate (SAR) in the exposed sample, a plastic Petri dish containing cell culture medium, full-wave 3-D electromagnetic simulations and temperature measurements were performed. For 1-W input power, the SAR values obtained at 1.8 GHz in the sample exposed in the TEM cell with the sealed or non-sealed aperture of 20-mm diameter were 1.1 W/kg and 23.6 W/kg, respectively. An excellent homogeneity of the SAR distribution was achieved when the aperture was sealed with the ITO layer. The performance of the delivery system was confirmed by microwave exposure and simultaneous observation of living cells.

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A Coplanar-Waveguide System for Cells Exposure During Electrophysiological Recordings

Cited by 27 publications

References 11 publications

Acute exposure to low‐level CW and GSM‐modulated 900 MHz radiofrequency does not affect Ba²⁺ currents through voltage‐gated calcium channels in rat cortical neurons

Acute exposure to low‐level CW and GSM‐modulated 900 MHz radiofrequency does not affect Ba²⁺ currents through voltage‐gated calcium channels in rat cortical neurons

High-accuracy adaptive modeling of the energy distribution of a meniscus-shaped cell culture in a Petri dish

Electromagnetic Analysis of an Aperture Modified Tem Cell Including an Ito Layer for Real-Time Observation of Biological Cells Exposed to Microwaves

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A Coplanar-Waveguide System for Cells Exposure During Electrophysiological Recordings

Cited by 27 publications

References 11 publications

Acute exposure to low‐level CW and GSM‐modulated 900 MHz radiofrequency does not affect Ba2+ currents through voltage‐gated calcium channels in rat cortical neurons

Acute exposure to low‐level CW and GSM‐modulated 900 MHz radiofrequency does not affect Ba2+ currents through voltage‐gated calcium channels in rat cortical neurons

High-accuracy adaptive modeling of the energy distribution of a meniscus-shaped cell culture in a Petri dish

Electromagnetic Analysis of an Aperture Modified Tem Cell Including an Ito Layer for Real-Time Observation of Biological Cells Exposed to Microwaves

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Product

Resources

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Acute exposure to low‐level CW and GSM‐modulated 900 MHz radiofrequency does not affect Ba²⁺ currents through voltage‐gated calcium channels in rat cortical neurons

Acute exposure to low‐level CW and GSM‐modulated 900 MHz radiofrequency does not affect Ba²⁺ currents through voltage‐gated calcium channels in rat cortical neurons