A cost-effective PCR–RFLP method for monitoring invasive American mink: preliminary field test in control areas of Spain

Dettori, Ettore Emanuele; Balestrieri, Alessandro; Román, Loreto García; Zapata-Pérez, Víctor Manuel; Ruiu, Angelo; Carcangiu, Laura; Robledano-Aymerich, Francisco

doi:10.1007/s12686-020-01183-3

Cited by 1 publication

(1 citation statement)

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“…DNA was extracted using the QIAamp® DNA Stool Mini Kit, after evaporating ethanol traces under vacuum. As spraints can be confused only with mink scats (Dettori et al 2021), and neither European mink (Mustela lutreola) nor American mink (Neovison vison) occur in the study area, we did not deem necessary to confirm species identification by mtDNA analysis.…”

Section: Genetic Analysismentioning

confidence: 99%

Faecal DNA-based genetic survey of a relict Eurasian otter (Lutra lutra) population (Sila Massif, S Italy)

Balestrieri

Gariano

Grandinetti

et al. 2022

Conservation Genet Resour

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Faecal DNA-based genetic analysis is a suitable tool for assessing both population size and genetic diversity of threatened and elusive species. We applied microsatellite analysis and mtDNA sequencing for investigating the southernmost Italian (Sila Massif, Calabria Region) population of the Eurasian otter (Lutra lutra). This relict population, filed as extinct in the mid-1980s, is currently expanding but still quite isolated. On the two main rivers hosting otters permanently since 2014, we collected 47 spraints, out of which 24 (51.1%) were successfully genotyped (on average 2.0 alleles per locus). Thirteen individuals were identified: seven females and three males (sex identification success of 76.9%). Population size was assessed as 16 individuals (13–22), corresponding to a density of 0.15 (0.13–0.21) ind/km. Successfully amplified mtDNA samples (N = 16) confirmed the occurrence of a haplotype—H10—which had been previously reported only for Southern Italy, bringing new evidence of the unicity of the Italian otter population. Although density values complied with those reported for the core area of otter Italian range, the small size and genetic isolation of this population require special attention. To assist the ongoing re-colonisation of the Sila Massif, habitat management should aim to enhance fish availability and connectivity with the core area.

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Section: Genetic Analysismentioning

confidence: 99%