2024
DOI: 10.1186/s13007-024-01154-x
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A Cotyledon-based Virus-Induced Gene Silencing (Cotyledon-VIGS) approach to study specialized metabolism in medicinal plants

Yongliang Liu,
Ruiqing Lyu,
Joshua J. Singleton
et al.

Abstract: Background Virus-induced gene silencing (VIGS) is widely used in plant functional genomics. However, the efficiency of VIGS in young plantlets varies across plant species. Additionally, VIGS is not optimized for many plant species, especially medicinal plants that produce valuable specialized metabolites. Results We evaluated the efficacy of five-day-old, etiolated seedlings of Catharanthus roseus (periwinkle) for VIGS. The seedlings were vacuum-in… Show more

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“…To improve the reliability of the results, we chose the classic reporter genes PDS and chlH for the successful establishment of passion fruit VIGS [19][20][21]. A gene related to chlorophyll synthesis, PechlH, was screened from the passion fruit genome and was hypothesised to be the catalytic subunit of magnesium chelatase, thus ensuring its activity, which may play an important regulatory role in chlorophyll synthesis [22].…”
Section: Discussionmentioning
confidence: 99%
“…To improve the reliability of the results, we chose the classic reporter genes PDS and chlH for the successful establishment of passion fruit VIGS [19][20][21]. A gene related to chlorophyll synthesis, PechlH, was screened from the passion fruit genome and was hypothesised to be the catalytic subunit of magnesium chelatase, thus ensuring its activity, which may play an important regulatory role in chlorophyll synthesis [22].…”
Section: Discussionmentioning
confidence: 99%