A Coumarin‐Based Fluorescent Probe for the Detection of Hypochlorite Ions and Its Applications in Test Paper and Cell Imaging

Abstract

A turn on fluorescence probe was designed cleverly and synthesized simply through the condensation reaction between coumarin derivative and barbiturate, for sensitively specific recognition of hypochlorite ion (ClO À ). This probe displayed a transient monitor process (about 5 min) to ClO À in a wide range of pH values (3-10), and the monitor limit could be estimated as low as 0.73μM. What's more, this probe could perform fluorescence imaging of ClO À in living cells, and make ClO À in aqueous solution appear on the convenient test strip visually.

BODIPY-Based Fluorescent Probes for Selective Visualization of Endogenous Hypochlorous Acid in Living Cells via Triazolopyridine Formation

“…Currently, fluorescence methods for HOCl detection using designed molecular probes have attracted high interest due to favorable spatiotemporal resolution in living systems. HOCl sensors have been developed in many fluorophores, such as naphthalimides [ 15 , 16 , 17 ], rhodamines [ 18 , 19 , 20 ], fluoresceins [ 21 , 22 , 23 ], coumarins [ 24 , 25 , 26 ], and BODIPYs [ 27 , 28 , 29 ]. As seen in the literature, most emissive HOCl sensors rely on a reaction-based strategy due to the powerful oxidizing nature of HOCl [ 30 , 31 , 32 ].…”

Research Progress of Small Molecule Fluorescent Probes for Detecting Hypochlorite

“…Figure 20. The reactions of probes 19, 20 and ClO − .Xie et al[48] proposed a simple fluorescence sensor 21 (Figure21) based on coumarin derivatives, which is obtained by the condensation reaction of barbiturates and coumarin derivatives. When a series of concentrations of ClO − (0-50 µM) titrate the probe solution, as the concentration increases, the UV absorption bands at 259 nm and 325 nm gradually decrease, while the UV absorption bands at 385 nm and 480 nm gradually increase.…”