A coupled diffusion-fluid pressure model to predict cell density distribution for cells encapsulated in a porous hydrogel scaffold under mechanical loading

Zhao, Feihu; Vaughan, Ted J.; Garrigle, Myles J. Mc; McNamara, Laoise M.

doi:10.1016/j.compbiomed.2017.08.003

Cited by 5 publications

(3 citation statements)

References 44 publications

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“…To reduce the computational cost, CFD analysis has been applied on one or a few unit-cells of the scaffold (Zhao et al 2017; Ali and Sen 2018a, b). For scaffolds with a regular pore geometry, it usually suffices to analyze only a small unit-cell of the sample, since the results for the full sample can be obtained by repetition of the unit-cell results (Marin and Lacroix 2015; Hendrikson et al 2017).…”

Section: Introductionmentioning

confidence: 99%

“…Performing a CFD analysis for the complete scaffold typically is inhibited by the high computational costs involved and by challenges in creating the complex meshes. The CFD analysis in such cases is typically limited to analyzing one or more relatively small representative volume elements (RVEs) (Sandino et al 2008; Stops et al 2010; Zhao et al 2017). The accuracy of such analyses will depend on many factors, e.g., homogeneity of the scaffold, prescribed boundary/loading conditions (Hu et al 2018).…”

Section: Introductionmentioning

confidence: 99%

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A multiscale computational fluid dynamics approach to simulate the micro-fluidic environment within a tissue engineering scaffold with highly irregular pore geometry

Zhao

Melke

Ito

et al. 2019

Biomech Model Mechanobiol

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Mechanical stimulation can regulate cellular behavior, e.g., differentiation, proliferation, matrix production and mineralization. To apply fluid-induced wall shear stress (WSS) on cells, perfusion bioreactors have been commonly used in tissue engineering experiments. The WSS on cells depends on the nature of the micro-fluidic environment within scaffolds under medium perfusion. Simulating the fluidic environment within scaffolds will be important for gaining a better insight into the actual mechanical stimulation on cells in a tissue engineering experiment. However, biomaterial scaffolds used in tissue engineering experiments typically have highly irregular pore geometries. This complexity in scaffold geometry implies high computational costs for simulating the precise fluidic environment within the scaffolds. In this study, we propose a low-computational cost and feasible technique for quantifying the micro-fluidic environment within the scaffolds, which have highly irregular pore geometries. This technique is based on a multiscale computational fluid dynamics approach. It is demonstrated that this approach can capture the WSS distribution in most regions within the scaffold. Importantly, the central process unit time needed to run the model is considerably low.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A multiscale computational fluid dynamics approach to simulate the micro-fluidic environment within a tissue engineering scaffold with highly irregular pore geometry

Zhao

Melke

Ito

et al. 2019

Biomech Model Mechanobiol

Self Cite

View full text Add to dashboard Cite

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“…In addition, the information obtained with CFD also allows to understand the influence exerted by the transport of nutrients on the growth of cells and the effect of cell proliferation on the tissue regeneration [7]. In this way, the effect of mechanical loading on three-dimensional scaffolds can be studied using CFD [8], and this is especially advantageous when designing bioreactors. Modelling with CFD helps to characterize the fluid flow and provides initial estimations.…”

Section: Introductionmentioning

confidence: 99%

Numerical Simulation by CFD of the Growth of Osteocytes within a Bioreactor

Paz¹,

Suárez²,

Vence³

et al. 2019

World Congress on Electrical Engineering and Computer Systems and Science

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For years bone grafts have been used to regenerate or replace damaged bones, treat fractures or bone diseases, which requires surgical intervention and all the risks and problems that this entails, such as: immune response, transmission of diseases etc. As a consequence of the limitations of this technique was born the engineering of bone tissues. The objective of this new type of tissue engineering is to carry out the cellular regeneration of the damaged bone tissue until completely re-establishing its functionality. This requires the creation of artificial porous structures (scaffolding or three-dimensional matrices) biocompatible in which bone cells (osteocytes) extracted from the patient's own tissue are cultured. Achieving adequate oxygen supply, a high cell density and a uniform distribution of cells over three-dimensional scaffolds has become a challenge in recent years. For this reason, a numerical model of osteocyte growth has been implemented in ANSYS Fluent. The model includes the oxygen and nutrient consumption of biomass, the effect of shear stress on cell proliferation and the specific growth rate of osteocytes. This model was evaluated in realistic threedimensional scaffolds inside a perfusion bioreactor and it has been proved that the cell proliferation is conditioned by the wall shear stress.

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Diffusion

Demi̇rel¹,

Gerbaud²

2019

Nonequilibrium Thermodynamics

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A coupled diffusion-fluid pressure model to predict cell density distribution for cells encapsulated in a porous hydrogel scaffold under mechanical loading

Cited by 5 publications

References 44 publications

A multiscale computational fluid dynamics approach to simulate the micro-fluidic environment within a tissue engineering scaffold with highly irregular pore geometry

A multiscale computational fluid dynamics approach to simulate the micro-fluidic environment within a tissue engineering scaffold with highly irregular pore geometry

Numerical Simulation by CFD of the Growth of Osteocytes within a Bioreactor

Diffusion

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