A covalent capillary coating of diazoresin and polyglycerol dendrimer for protein analysis using capillary electrophoresis

Abstract

Overcoming proteins adsorption on the inner surface of capillary has attracted increasing attention recently. By using the unique photochemistry reaction of diazoresin (DR), a new covalent capillary coating was prepared on the fused-silica capillary through layer-by-layer self-assembly of DR with polyglycerol (PG) dendrimer. The separation performance of covalently DR/PG-dendrimer coated capillary noticeably exceeded the bare capillary and the noncovalently linked DR/PG-dendrimer capillary. A baseline separation of lysozyme, myoglobin, bovine serum albumin, and ribonuclease A was achieved using CE within 20 min. Besides, the covalently linked DR/PG-dendrimer coating has the remarkable stability and reproducibility. Especially, compared with the traditional method which use highly toxic and moisture-sensitive silane coupling agent, this method seems to be a simple and environmental friendly way to prepare the covalently coated capillaries for CE.

Controllable and high‐performance immobilized enzyme reactor: DNA‐directed immobilization of multienzyme in polyamidoamine dendrimer‐functionalized capillaries

“…However, most open‐tubular CE‐integrated IMERs are limited to the immobilization of single enzyme, which significantly confines their broad practical application. In the past two decades, there have been several attempts to develop the CE‐integrated IMERs for multienzyme immobilization . Yang et al.…”

“…However, most open-tubular CE-integrated IMERs are limited to the immobilization of single enzyme, which significantly confines their broad practical application. In the past two decades, there have been several attempts to develop the CE-integrated IMERs for multienzyme immobilization [22][23][24][25][26][27][28][29][30]. Yang et al constructed a double-enzyme, coimmobilized, CE-integrated IMER for the detection of glutamic acid; glutamate dehydrogenase and glutamate pyruvate transaminase were directly immobilized on the inner wall of a capillary by covalent bonding [31].…”

Screening and investigation of a short antimicrobial peptide: AVGAV

“…Compared with traditional crosslinking agents, the photo-crosslinking reaction based on diazo resin can more easily form covalent bonds between microspheres and cell membranes, and the damage to cell membranes during the covalent bond formation process is relatively small. 25 The morphology and size of the self-made silica microspheres were characterized by SEM. As shown in Fig.…”