A CRISPR‐Cas9‐mediated domain‐specific base‐editing screen enables functional assessment of ACCase variants in rice

Liu, Xiaoshuang; Qin, Ruiying; Li, Juan; Liao, Shengxiang; Shan, Tiaofeng; Xu, Ruihua; Wu, De-Xiang; Wei, Pengcheng

doi:10.1111/pbi.13348

Cited by 81 publications

(48 citation statements)

References 10 publications

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“…ACCase is responsible for catalyzing the initial step of the biosynthesis of fatty acid. The loss-of-function modification in ACCase leads to severe developmental arrest in plants [ 115 , 143 ]. The identified ACCase -inhibiting herbicide resistant mutations arise in carboxyltransferase domain that directly interacts with herbicides [ 144 ].…”

Section: Mechanism and Approaches For Development Of Herbicide Resistant Plants/cropsmentioning

confidence: 99%

“…More importantly, genome editing, specially programmable base editing, presents heritable, targeted modifications leading to transgene-free HR crops [ 40 , 115 , 122 ] that are genetically non-distinguishable to that of the plants created by traditional mutagenesis [ 151 ]. Moreover, base editing offers multiple amino acids substitution of targeted genes that confers resistance to multiple herbicides [ 26 ].…”

Section: Advantages and Disadvantages Of Genome Editing In Herbicide Resistancementioning

confidence: 99%

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Herbicide Resistance: Another Hot Agronomic Trait for Plant Genome Editing

Hussain

Ding

Alariqi

et al. 2021

Plants

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Weeds have continually interrupted crop plants since their domestication, leading to a greater yield loss compared to diseases and pests that necessitated the practice of weed control measures. The control of weeds is crucial to ensuring the availability of sufficient food for a rapidly increasing human population. Chemical weed control (herbicides) along with integrated weed management (IWM) practices can be the most effective and reliable method of weed management programs. The application of herbicides for weed control practices calls for the urgency to develop herbicide-resistant (HR) crops. Recently, genome editing tools, especially CRISPR-Cas9, have brought innovation in genome editing technology that opens up new possibilities to provide sustainable farming in modern agricultural industry. To date, several non-genetically modified (GM) HR crops have been developed through genome editing that can present a leading role to combat weed problems along with increasing crop productivity to meet increasing food demand around the world. Here, we present the chemical method of weed control, approaches for herbicide resistance development, and possible advantages and limitations of genome editing in herbicide resistance. We also discuss how genome editing would be effective in combating intensive weed problems and what would be the impact of genome-edited HR crops in agriculture.

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Section: Mechanism and Approaches For Development Of Herbicide Resistant Plants/cropsmentioning

confidence: 99%

Section: Advantages and Disadvantages Of Genome Editing In Herbicide Resistancementioning

confidence: 99%

Herbicide Resistance: Another Hot Agronomic Trait for Plant Genome Editing

Hussain

Ding

Alariqi

et al. 2021

Plants

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“…Base editors were also employed to disrupt genes in plants by creating early stop codons or inducing transcript mis-splicing ( Kang et al., 2018 , Xue et al., 2018 , Li et al., 2019b , Veillet et al., 2019 ). In addition, together with a single guide RNA (sgRNA) library, base editors can generate high-density substitutions in the target region, thus facilitating the directed evolution of plant genes ( Kuang et al., 2020 , Li et al., 2020a , Liu et al., 2020 ). Base editors have been extensively optimized to achieve better application in plants.…”

Section: Introductionmentioning

confidence: 99%

Development of Plant Prime-Editing Systems for Precise Genome Editing

Liu

et al. 2020

Plant Communications

Self Cite

166

123

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Prime-editing systems have the capability to perform efficient and precise genome editing in human cells. In this study, we first developed a plant prime editor 2 (pPE2) system and test its activity by generating a targeted mutation on an HPT -ATG reporter in rice. Our results showed that the pPE2 system could induce programmable editing at different genome sites. In transgenic T 0 plants, pPE2-generated mutants occurred with 0%–31.3% frequency, suggesting that the efficiency of pPE2 varied greatly at different genomic sites and with prime-editing guide RNAs of diverse structures. To optimize editing efficiency, guide RNAs were introduced into the pPE2 system following the PE3 and PE3b strategy in human cells. However, at the genomic sites tested in this study, pPE3 systems generated only comparable or even lower editing frequencies. Furthemore, we developed a surrogate pPE2 system by incorporating the HPT -ATG reporter to enrich the prime-edited cells. The nucleotide editing was easily detected in the resistant calli transformed with the surrogate pPE2 system, presumably due to the enhanced screening efficiency of edited cells. Taken together, our results indicate that plant prime-editing systems we developed could provide versatile and flexible editing in rice genome.

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“…Combined with sgRNA libraries, the base-editing toolbox holds great promise to drive CRISPR-directed in planta evolution of proteins by generating many targeted mutations in a whole gene or specific sequence-encoding domains, allowing the identification of new key amino acid(s) associated with agronomic traits ( Capdeville et al., 2020 ). So far, CRISPR-directed in planta evolution has been applied to confer herbicide resistance through amino acid substitutions in OsALS1 and OsACC genes ( Li et al., 2020b ; Kuang et al., 2020 ; Liu et al., 2020 ), but there is no doubt that this strategy could be used for ecological-friendly purposes such as the development of pathogen-resistant crops.…”

Section: Precision Editing: Refining the Tools?mentioning

confidence: 99%

Precision Breeding Made Real with CRISPR: Illustration through Genetic Resistance to Pathogens

Veillet

Durand

Kroj

et al. 2020

Plant Communications

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Since its discovery as a bacterial adaptive immune system and its development for genome editing in eukaryotes, the CRISPR technology has revolutionized plant research and precision crop breeding. The CRISPR toolbox holds great promise in the production of crops with genetic disease resistance to increase agriculture resilience and reduce chemical crop protection with a strong impact on the environment and public health. In this review, we provide an extensive overview on recent breakthroughs in CRISPR technology, including the newly developed prime editing system that allows precision gene editing in plants. We present how each CRISPR tool can be selected for optimal use in accordance with its specific strengths and limitations, and illustrate how the CRISPR toolbox can foster the development of genetically pathogen-resistant crops for sustainable agriculture.

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A CRISPR‐Cas9‐mediated domain‐specific base‐editing screen enables functional assessment of ACCase variants in rice

Cited by 81 publications

References 10 publications

Herbicide Resistance: Another Hot Agronomic Trait for Plant Genome Editing

Herbicide Resistance: Another Hot Agronomic Trait for Plant Genome Editing

Development of Plant Prime-Editing Systems for Precise Genome Editing

Precision Breeding Made Real with CRISPR: Illustration through Genetic Resistance to Pathogens

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