A CRISPR/dCas9 toolkit for functional analysis of maize genes

Gentzel, Irene; Park, Chan Ho; Bellizzi, Maria; Xiao, Guiqing; Gadhave, Kiran R.; Murphree, Colin A.; Yang, Qin; LaMantia, Jonathan; Redinbaugh, Margaret G.; Balint‐Kurti, Peter; Sit, Tim L.; Wang, Guo‐Liang

doi:10.1186/s13007-020-00675-5

Cited by 26 publications

(13 citation statements)

References 46 publications

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“…The new addition of CRISPR-interference (CRISPRi) and CRISPR-mediated activation (CRISPRa) in the CRISPR family, using the dCas9 toolkit, as developed in maize [127], has great potential for further improvement of genome engineering. Targeting the core promoter of a gene by editing technologies could be a reliable approach for fine-tuning of any desired gene expression, unearthing new avenues for breeding improved rice grain quality.…”

Section: Discussionmentioning

confidence: 99%

Applications and Potential of Genome-Editing Systems in Rice Improvement: Current and Future Perspectives

et al. 2021

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Food crop production and quality are two major attributes that ensure food security. Rice is one of the major sources of food that feeds half of the world’s population. Therefore, to feed about 10 billion people by 2050, there is a need to develop high-yielding grain quality of rice varieties, with greater pace. Although conventional and mutation breeding techniques have played a significant role in the development of desired varieties in the past, due to certain limitations, these techniques cannot fulfill the high demands for food in the present era. However, rice production and grain quality can be improved by employing new breeding techniques, such as genome editing tools (GETs), with high efficiency. These tools, including clustered, regularly interspaced short palindromic repeats (CRISPR) systems, have revolutionized rice breeding. The protocol of CRISPR/Cas9 systems technology, and its variants, are the most reliable and efficient, and have been established in rice crops. New GETs, such as CRISPR/Cas12, and base editors, have also been applied to rice to improve it. Recombinases and prime editing tools have the potential to make edits more precisely and efficiently. Briefly, in this review, we discuss advancements made in CRISPR systems, base and prime editors, and their applications, to improve rice grain yield, abiotic stress tolerance, grain quality, disease and herbicide resistance, in addition to the regulatory aspects and risks associated with genetically modified rice plants. We also focus on the limitations and future prospects of GETs to improve rice grain quality.

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Section: Discussionmentioning

confidence: 99%

Applications and Potential of Genome-Editing Systems in Rice Improvement: Current and Future Perspectives

et al. 2021

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“…In addition to the function of genomic sequence editing, CRISPR/Cas9 also has been adapted for transcriptional activation (CRISPRa) or inhibition (CRISPRi) at endogenous genomic loci. A nuclease-deficient Cas9 (dCas9) fused with an activation domain can facilitate the transcriptional activation, while a dCas9 fused with a repression domain can inhibit gene expression (Gentzel et al, 2020). Actually, the CRISPR/ Cas system is constantly evolving, besides Cas9, other endonucleases were also discovered, such as Cpf1 (also known as Cas12a), Cas13, and Cas14 (Manghwar et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

Applications of CRISPR/Cas9-based genome editing in the plant biology

Zheng¹,

Li²,

Ye³

et al. 2021

Turk J Bot

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Clustered regularly interspaced short palindromic repeats/CRISPR-associated proteins (CRISPR/Cas) is an acquired immune system found in bacteria and archaea that can specifically silence or degrade a foreign single or double strand nucleic acid to protect it from infection. In recent years, the CRISPR/Cas9 system has rapidly been evolved into a genome editing technology, in which the Cas9 endonuclease can be targeted to specific DNA sequences by guide RNAs (gRNAs) that are easily programmable. Due to simplicity, specificity and high efficiency, CRISPR/Cas9 is gradually replacing other gene editing technologies and has been implemented in basic and applied plant sciences to boost yield, regulate metabolic process, and increase stress resistance in different varieties. In current review, we introduced its application scope in scientific research and practical application. We summarized the procedure of target plant generation by CRISPR/Cas9 method. We mainly reviewed the applications of CRISPR/Cas9 and its recent advances in model plants and other crop plants, attempting to provide a related general information to researchers. Further, we also included the inadequacies and concerns of CRISPR/Cas9 that have emerged so far.

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“…[ 149 ] For targeted repression of transcription in plants, a CRISPR interference tools were developed by dCas9 fusion with a single or triple SUPERMAN repression domain X motif. [ 142,150 ] Moreover, the CRISPR‐Cas13 RNA endonucleases showed activity in plants, being repurposed for targeted post‐transcriptional RNA silencing of endogenous genes and inducing immunity against RNA viruses. [ 151–154 ]…”

Section: Tools For Genome Editing In Plantsmentioning

confidence: 99%

If Mendel Was Using CRISPR: Genome Editing Meets Non‐Mendelian Inheritance

Hudzieczek

Hobza

Cápal

et al. 2022

Adv Funct Materials

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On July 20 this year, 200th anniversary of the birth of Gregor Mendel, an Augustinian monk who laid the foundations of genetics, is celebrated. His findings provided the stepping stone for rational crop improvement based on cross breeding and selection. While this approach contributed to the development of superior varieties, its potential is limited by genetic variability available within a species and its relatives. Even though physical and chemical mutagenesis enabled the induction of new genetic variability on a large scale, random mutagenesis did not allow targeting particular genome loci. This became feasible only after the development of genome editing tools in the beginning of the 21st century and the ability to precisely edit plant genomes opened a new era in plant research and crop breeding. Mendel probably did not consider a possibility of directly modifying genetic information and the advantages which genetic engineering can bring to society. However, the basic principles of inheritance he discovered are indispensable for any effort to develop improved varieties. In this review, recent advances in plant genome editing are summarized with emphasis on non-Mendelian inheritance in the light of the need to produce more food for the growing human population.

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A CRISPR/dCas9 toolkit for functional analysis of maize genes

Cited by 26 publications

References 46 publications

Applications and Potential of Genome-Editing Systems in Rice Improvement: Current and Future Perspectives

Applications and Potential of Genome-Editing Systems in Rice Improvement: Current and Future Perspectives

Applications of CRISPR/Cas9-based genome editing in the plant biology

If Mendel Was Using CRISPR: Genome Editing Meets Non‐Mendelian Inheritance

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