2023
DOI: 10.1039/d2lc00852a
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A critical review of microfluidic systems for CRISPR assays

Abstract: We review recent advances in CRISPR-based nucleic acid detection using microfluidic devices and discuss the capabilities, limitations, and potential of this emerging technology.

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Cited by 25 publications
(7 citation statements)
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“…In this modality, fluorogenic enzymes are directly activated by one-to-one binding to target biomolecules, allowing indirect detection of target molecules from the fluorescent signal generated by the enzymatic reaction. Recently, nucleic acid cleavage enzymes such as CRISPR–Cas proteins, which are activated upon binding to specific nucleic acid sequences, have been widely adopted in biomedical applications 18 , 19 . Digital bioanalysis using these enzymes has attracted great attention in the diagnosis of infectious diseases, including COVID-19, as an innovative genetic testing method that bypasses the amplification process of genetic material 11 , 12 , 20 23 .…”
Section: Resultsmentioning
confidence: 99%
“…In this modality, fluorogenic enzymes are directly activated by one-to-one binding to target biomolecules, allowing indirect detection of target molecules from the fluorescent signal generated by the enzymatic reaction. Recently, nucleic acid cleavage enzymes such as CRISPR–Cas proteins, which are activated upon binding to specific nucleic acid sequences, have been widely adopted in biomedical applications 18 , 19 . Digital bioanalysis using these enzymes has attracted great attention in the diagnosis of infectious diseases, including COVID-19, as an innovative genetic testing method that bypasses the amplification process of genetic material 11 , 12 , 20 23 .…”
Section: Resultsmentioning
confidence: 99%
“…As the synthesis of RNA oligos is expensive, these properties will also contribute to reducing the overall cost of the assay. We envision that the integration of microfluidics technology will further advance UNIVERSE toward a valid point‐of‐care testing tool [5b,d,24] …”
Section: Discussionmentioning
confidence: 99%
“…Recently, CRISPR-Cas has attracted attention as a new genetic test for the detection of viral genomes [ 9 , 10 ]. Cas12a and Cas13a, which are widely used in genetic testing, form a binary complex with CRISPR-RNA (crRNA), which consequently form a specific ternary complex with the target DNA or RNA, having complementary sequences to crRNA.…”
Section: Satori: a Genetic Test Based On Digital Bioanalysis Using Cr...mentioning
confidence: 99%