A Critical Review on Biotechnological Interventions for Production and Yield Enhancement of Secondary Metabolites in Hairy Root Cultures

Halder, Mihir; Roychowdhury, Dipasree; Jha, Sumita

doi:10.1007/978-981-13-2562-5_2

Cited by 26 publications

(18 citation statements)

References 88 publications

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“…use of transgenic or nontransgenic HRCs), the production of specialized metabolites has been successfully improved by optimizing the growth conditions (e.g. carbon source, aeration, pH, light/dark, culture medium composition), selection of the clone (Sevón et al, 1998), and/or by paying a particular attention to the selection of adapted elicitors (Wang and Wu, 2013;Perassolo et al, 2017;Halder et al, 2018). The expression of specialized metabolites in HRCs, as in all plant-based production systems, requires the identification of the most appropriate elicitors and administration scheme.…”

Section: Production Of Specialized Metabolitesmentioning

confidence: 99%

Hairy Root Cultures—A Versatile Tool With Multiple Applications

Gutierrez-Valdes

Häkkinen

Lemasson³

et al. 2020

Front. Plant Sci.

188

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Hairy roots derived from the infection of a plant by Rhizobium rhizogenes (previously referred to as Agrobacterium rhizogenes) bacteria, can be obtained from a wide variety of plants and allow the production of highly diverse molecules. Hairy roots are able to produce and secrete complex active glycoproteins from a large spectrum of organisms. They are also adequate to express plant natural biosynthesis pathways required to produce specialized metabolites and can benefit from the new genetic tools available to facilitate an optimized production of tailor-made molecules. This adaptability has positioned hairy root platforms as major biotechnological tools. Researchers and industries have contributed to their advancement, which represents new alternatives from classical systems to produce complex molecules. Now these expression systems are ready to be used by different industries like pharmaceutical, cosmetics, and food sectors due to the development of fully controlled large-scale bioreactors. This review aims to describe the evolution of hairy root generation and culture methods and to highlight the possibilities offered by hairy roots in terms of feasibility and perspectives.

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Section: Production Of Specialized Metabolitesmentioning

confidence: 99%

Hairy Root Cultures—A Versatile Tool With Multiple Applications

Gutierrez-Valdes

Häkkinen

Lemasson³

et al. 2020

Front. Plant Sci.

188

View full text Add to dashboard Cite

show abstract

“…The effects of MJ and SA elicitation at different time points (6,12,24, and 48 h) on accumulation of morphinan alkaloids (thebaine, morphine, and codeine) and the relative expression level of six main regulatory genes of the morphinan pathway (COR, SalAT, SalR, T6ODM, CODM and Salsyn) was assessed in HRCs of Papaver orientale [69]. The investigators found that the accumulation of morphinan alkaloids under elicitation through MJ and/or SA were directly related to the expression levels of key genes in the morphinan pathway at different time points.…”

Section: Effect Of Signal Molecules On Sm Productionmentioning

confidence: 99%

“…Various biotechnological strategies such as screening and selection of high yielding line, optimization of culture media composition and physical parameters, precursor feeding, elicitation, large scale cultivation in bioreactor system, hairy root culture (HRC), metabolic engineering, plant cell immobilization, biotransformation, etc. have been assayed to evaluate their effectiveness towards enhancement of SM production utilizing in vitro plant cell culture and/or organ culture of different plant species .…”

Section: Introductionmentioning

confidence: 99%

Elicitation: A biotechnological tool for enhanced production of secondary metabolites in hairy root cultures

Halder

Sarkar

Jha

2019

Engineering in Life Sciences

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238

116

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Elicitation is a possible aid to overcome various difficulties associated with the large‐scale production of most commercially important bioactive secondary metabolites from wild and cultivated plants, undifferentiated or differentiated cultures. Secondary metabolite accumulation in vitro or their efflux in culture medium has been elicited in the undifferentiated or differentiated tissue cultures of several plant species by the application of a low concentration of biotic and abiotic elicitors in the last three decades. Hairy root cultures are preferred for the application of elicitation due to their genetic and biosynthetic stability, high growth rate in growth regulator‐free media, and production consistence in response to elicitor treatment. Elicitors act as signal, recognized by elicitor‐specific receptors on the plant cell membrane and stimulate defense responses during elicitation resulting in increased synthesis and accumulation of secondary metabolites. Optimization of various parameters, such as elicitor type, concentration, duration of exposure, and treatment schedule is essential for the effectiveness of the elicitation strategies. Combined application of different elicitors, integration of precursor feeding, or replenishment of medium or in situ product recovery from the roots/liquid medium with the elicitor treatment have showed improved accumulation of secondary metabolites due to their synergistic effect. This is a comprehensive review about the progress in the elicitation approach to hairy root cultures from 2010 to 2019 and the information provided is valuable and will be of interest for scientists working in this area of plant biotechnology.

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“…Several studies on micropropagation, somatic embryogenesis and synthetic seed production were conducted for sustainable production of S. chirayita plants throughout the year (Kumar and Staden 2016). Agrobacterium rhizogenes induced hairy root technology has been established in large number of species for the production of secondary metabolites (Halder et. al.…”

Section: Introductionmentioning

confidence: 99%

Agrobacterium rhizogenes Mediated Transformation of the Critically Endangered Species, Swertia chirayita

Samaddar

Sarkar

Jha

2019

Plant Tissue Cult. & Biotech.

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Agrobacterium rhizogenes-mediated genetic transformation for the establishment of root culture lines was developed successfully in the critically endangered medicinal plant Swertia chirayita (Roxb.) H. Karst. The key factor for the successfull development of hairy root culture lines was the use of whole micropropagated plants as excised explants of any type did not show any response following infection with A. rhizogenes strains. Maximum root induction frequency (25 ± 4.4%) with 2 - 9 roots per node/intermodal cut site was obtained following infection with A. rhizogenes strain LBA9402. Spontaneous regeneration of shoot buds was observed in six 9402-transformed root lines maintained in phytohormone free N/5 basal medium. The shoot buds developed for regenerating whole rooted Ri-transformed plants in phytohormone free basal medium. Integration of rolA, rolB, rolC and rolD genes of TL-DNA was confirmed in Ri-transformed root lines and plants by PCR analysis. Ten fast growing Ri-transformed root lines were screened for production of principal secoiridoids, characteristics of the parent plants. The swertiamerin content varied from 0.042 to 0.207% in the transformed root lines after 4 weeks of culture in N/5 basal medium. Ri-transformed plants showed enhanced accumulation of swertiamerin as compared to non-transformed plants of similar age. This is the first report of swertiamerin production in hairy root cultures and transformed plants of S. chirayita thereby providing new avenue for large-scale production of secondary metabolites of S. chirayita.

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A Critical Review on Biotechnological Interventions for Production and Yield Enhancement of Secondary Metabolites in Hairy Root Cultures

Cited by 26 publications

References 88 publications

Hairy Root Cultures—A Versatile Tool With Multiple Applications

Hairy Root Cultures—A Versatile Tool With Multiple Applications

Elicitation: A biotechnological tool for enhanced production of secondary metabolites in hairy root cultures

Agrobacterium rhizogenes Mediated Transformation of the Critically Endangered Species, Swertia chirayita

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