A critical review on use of Agrobacterium rhizogenes and their associated binary vectors for plant transformation

Bahramnejad, Bahman; Naji, Mohammad; Bose, Rahul; Jha, Sumita

doi:10.1016/j.biotechadv.2019.06.004

Cited by 65 publications

(60 citation statements)

References 199 publications

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“…R. rhizogenes previously known as Agrobacterium rhizogenes, is a soil-borne gram-negative bacteria that causes hairy roots in plants. In most plant species, cells transformed with R. rhizogenes and its Ri plasmid differentiate into transformed roots, serving as a visual marker for markerfree screening and selection (Young et al, 2001;Bahramnejad et al, 2019). A comprehensive description of R. rhizogenes strains, binary vectors, and plants transformed using them is provided by (Bahramnejad et al, 2019).…”

Section: The Genome Editing Processmentioning

confidence: 99%

Principles, Applications, and Biosafety of Plant Genome Editing Using CRISPR-Cas9

2020

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The terms genome engineering, genome editing, and gene editing, refer to modifications (insertions, deletions, substitutions) in the genome of a living organism. The most widely used approach to genome editing nowadays is based on Clustered Regularly Interspaced Short Palindromic Repeats and associated protein 9 (CRISPR-Cas9). In prokaryotes, CRISPR-Cas9 is an adaptive immune system that naturally protects cells from DNA virus infections. CRISPR-Cas9 has been modified to create a versatile genome editing technology that has a wide diversity of applications in medicine, agriculture, and basic studies of gene functions. CRISPR-Cas9 has been used in a growing number of monocot and dicot plant species to enhance yield, quality, and nutritional value, to introduce or enhance tolerance to biotic and abiotic stresses, among other applications. Although biosafety concerns remain, genome editing is a promising technology with potential to contribute to food production for the benefit of the growing human population. Here, we review the principles, current advances and applications of CRISPR-Cas9-based gene editing in crop improvement. We also address biosafety concerns and show that humans have been exposed to Cas9 protein homologues long before the use of CRISPR-Cas9 in genome editing.

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Section: The Genome Editing Processmentioning

confidence: 99%

Principles, Applications, and Biosafety of Plant Genome Editing Using CRISPR-Cas9

2020

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“…New developments in genetic engineering have allowed much greater control over the transfer of foreign genes to plant cells. A number of vectors can now be used in transgenesis [28,49] that enable different marker and reporter genes and restriction sites to be chosen; they also allow high copy numbers of the binary vectors already in common use in biotechnological laboratories in bacterial cells. Agrobacteriumbased transformation typically proceeds according to the following process: first, naturally-occurring oncogenes are removed to deprive the bacterium of pathogenicity; such a strain is often referred to as disarmed.…”

Section: Binary Vectors As a Basic Tool In Plant Genetic Transformationmentioning

confidence: 99%

“…One of the key strategies enabling the overproduction of valuable plant secondary metabolites in in vitro cultures is the manipulation of existing metabolic pathways by overexpressing or silencing selected elements involved in their biosynthesis [24][25][26][27]. A wide range of plant vectors have been designed that allow the simple and quick introduction of synthetic expression cassettes, allowing easier and more effective creation of in vitro transgenic plant cultures [28][29][30]. Several studies confirm that such modulation of metabolic pathways increases the yield of naturally-occurring biologically-active compounds with potential use in medicine [31][32][33].…”

Section: Introductionmentioning

confidence: 99%

Transgenesis as a Tool for the Efficient Production of Selected Secondary Metabolites from Plant in Vitro Cultures

Kowalczyk

Wieczfińska

Skała

et al. 2020

Plants

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The plant kingdom abounds in countless species with potential medical uses. Many of them contain valuable secondary metabolites belonging to different classes and demonstrating anticancer, anti-inflammatory, antioxidant, antimicrobial or antidiabetic properties. Many of these metabolites, e.g., paclitaxel, vinblastine, betulinic acid, chlorogenic acid or ferrulic acid, have potential applications in medicine. Additionally, these compounds have many therapeutic and health-promoting properties. The growing demand for these plant secondary metabolites forces the use of new green biotechnology tools to create new, more productive in vitro transgenic plant cultures. These procedures have yielded many promising results, and transgenic cultures have been found to be safe, efficient and cost-effective sources of valuable secondary metabolites for medicine and industry. This review focuses on the use of various in vitro plant culture systems for the production of secondary metabolites.

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“…Besides A. tumefaciens , Agrobacterium rhizogenes has become a popular agent for transforming plant genomes (Ozyigit et al, 2013). A. rhizogenes is a soil‐borne bacterium infecting many angiosperms and causing them to produce a copious number of roots which became known as the “hairy root syndrome” (Bahramnejad et al, 2019). Like A. tumefaciens , A. rhizogenes transfers a segment of DNA known as T‐DNA into its hosts.…”

Section: Introductionmentioning

confidence: 99%

A highly efficient protocol for transforming Cuscuta reflexa based on artificially induced infection sites

2020

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The parasitic plant genus Cuscuta is notoriously difficult to transform and to propagate or regenerate in vitro. With it being a substantial threat to many agroecosystems, techniques allowing functional analysis of gene products involved in host interaction and infection mechanisms are, however, in high demand. We set out to explore whether Agrobacterium‐mediated transformation of different plant parts can provide efficient alternatives to the currently scarce and inefficient protocols for transgene expression in Cuscuta. We used fluorescent protein genes on the T‐DNA as markers for transformation efficiency and transformation stability. As a result, we present a novel highly efficient transformation protocol for Cuscuta reflexa cells that exploits the propensity of the infection organ to take up and express transgenes with the T‐DNA. Both, Agrobacterium rhizogenes and Agrobacterium tumefaciens carrying binary transformation vectors with reporter fluorochromes yielded high numbers of transformation events. An overwhelming majority of transformed cells were observed in the cell layer below the adhesive disk’s epidermis, suggesting that these cells are particularly susceptible to infection. Cotransformation of these cells happens frequently when Agrobacterium strains carrying different constructs are applied together. Explants containing transformed tissue expressed the fluorescent markers in in vitro culture for several weeks, offering a future possibility for development of transformed cells into callus. These results are discussed with respect to the future potential of this technique and with respect to the special characteristics of the infection organ that may explain its competence to take up the foreign DNA.

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A critical review on use of Agrobacterium rhizogenes and their associated binary vectors for plant transformation

Cited by 65 publications

References 199 publications

Principles, Applications, and Biosafety of Plant Genome Editing Using CRISPR-Cas9

Principles, Applications, and Biosafety of Plant Genome Editing Using CRISPR-Cas9

Transgenesis as a Tool for the Efficient Production of Selected Secondary Metabolites from Plant in Vitro Cultures

A highly efficient protocol for transforming Cuscuta reflexa based on artificially induced infection sites

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