Mosquitoes are most dangerous insect in the world and they cause many diseases to birds, animals and humans. The control of mosquitoes using plant product is more effectiveness. The current study evaluates the larvicidal bioassay of Chromolaena odorata leaf extracts carried out against fourth instar larvae of Culex quinquefasciatus. The extracts were obtained from solvent such as Petroleum ether, Chloroform, and Ethanol. Extracts from C. odorata were tested for larvicidal activity at levels ranging from 0.625ppm, 1.25 ppm, 2.5 ppm, and 5 ppm. The mortality rate of the larvae was determined 24 hours, 48 hours, and 72 hours. The calculation of LC50 value of extracts was calculated after 48hrs exposure. After a 48-hrs exposure, the LC50 value of the extracts was calculated. After 48 hrs of incubation, the C. odorata Petroleum ether extract showed 100% mortality. C. quinquefasciatus was more susceptible to plant extracts. The crude extracts of C. odorata found to better results on vector control it may be active phytochemicals present in this extract to be explored and also plant-derived compounds have significant potential in mosquito vectors control programme.