A critique of avianCHD‐based molecular sexing protocols illustrated by a Z‐chromosome polymorphism detected in auklets

Dawson, Deborah A.; Darby, Steven; Hunter, Fiona M.; Krupa, Andrew P.; Jones, Ian L.; Burke, Terry

doi:10.1046/j.1471-8278.2001.00060.x

Cited by 103 publications

(103 citation statements)

References 11 publications

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“…These sizes were slightlydifferent compare to the finding reported by Dawson et al (2001), using the primer to sex four species of auklet (Z=600; W=430). In other had using primer set P2/P8 obtained amplicon size from chromosome W = 393 bp and chromosomes Z =351 bp ( Figure 1C).…”

Section: Resultscontrasting

confidence: 77%

SEX DETERMINATION OF BALI STARLING (Leucopsar rothschildi) USING MOLECULAR SEXING

Wirastika

Yuda

Zahida

2015

KLS

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Bali Starling (Leucopsar rothschildi) are monomorphic at the age of nestling. For the conservation of bird it important is to determine its sex at the earlier stage. Conventional methods have limitations. This study applied PCR-based molecular sexing to answer this issue. This study aimed to obtain the most effective molecular primers to identify the sex of Bali starling. The most common used combination of P2/P8, 2550F/ 2718R and 1237L/1272H primers, which amplify CHD1 gene (Chromo-helicase-DNA-binding) were evaluated. DNA samples were obtained from secondary wing feathers of young Bali Starling. Separation in agarose gel electrophoresis of PCR products showed that the three primers were successfully amplified the samples with different degrees of success, that was 90% (P2/P8), 86.7% (2550F/2718R), and 73.3% (1237L/1272H), respectively. However, only the combination of P2/P4 and 2550F/2718R primers was able to sex Bali Starling based on observation of PCR products on agarose gel. The sizes of the genes were slightly different with those reported on previous studies. Most of the results of molecular sexing were in accordance with the sex based on morphological characters. Keywords : Bali starling, Leucopsar rothschildi, molecular sexing, CHD gene

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Section: Resultscontrasting

confidence: 77%

SEX DETERMINATION OF BALI STARLING (Leucopsar rothschildi) USING MOLECULAR SEXING

Wirastika

Yuda

Zahida

2015

KLS

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“…1). This polymorphic Z-product has also been found in 4 species of male auklets (Dawson et al 2001).…”

Section: Sex Determinationmentioning

confidence: 54%

Breeding ecology and bias in offspring sex ratio in little grassbirds (Megalurus gramineus)

et al. 2003

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Little grassbirds (Megalurus gramineus) are small, sexually monomorphic passerines that live in reed beds, lignum swamps and salt marshes in southern Australia. The breeding biology and patterns of sex allocation of the little grassbird were investigated over a single breeding season. Our observations of this species in the Edithvale Wetland Reserve revealed a highly male-biased population sex ratio, with some breeding territories containing several additional males. Nevertheless, there was little compelling evidence that little grassbirds breed cooperatively. The growth rates of male and female nestlings were similar and, as predicted by theory, there was no overall primary sex ratio bias. However, the primary sex ratio was female-biased early in the breeding season and became increasingly male-biased later in the breeding season.

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“…This method is subject to technical failure, resulting from polymorphism in the hybridization sites of primers, preferential amplification or polymorphism of Z chromosome (Dawson et al, 2001;Robertson and Gemmell, 2006). An additional molecular test, to evaluate the incidence of this technical failure in detecting sex by PCR, could not be performed within the scope of this study.…”

Section: Discussionmentioning

confidence: 99%

Using PCR-RFLP for sexing of the endangered Galápagos petrel (Pterodroma phaeopygia)

Patiño¹,

Cruz²,

Martı́nez³

et al. 2013

Genet. Mol. Res.

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ABSTRACT. Pterodroma phaeopygia is a critically endangered avian species of the Galápagos Islands. This bird is sexually monomorphic, making it difficult to identify the sex. This information, however, is relevant to studies of behavior, ecology, and management of wild or captive populations. Here, we aimed to implement a molecular approach for determining sex in this petrel. DNA was extracted from the blood and the feathers of 24 adult P. phaeopygia, with samples from a female and a male Gallus gallus for comparison. We amplified the cromo-helicase DNA binding protein 1 (CHD-1) gene by PCR, using primers P2 and P8. Allele CHD-1W is unique to females and CHD-1Z occurs in both sexes. We then digested these PCR products using the restriction enzyme HaeIII. The PCR amplified a 400-bp product for both alleles. The digestion of the G. gallus, amplicons split the CHD-1Z allele into two fragments (of 320 and 80 bp), while CHD-1W 4761 ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 12 (4): 4760-4767 (2013) Sexing the Galapagos petrel remained intact. Thus, the male exhibited two bands (digested CHD-1Z) and the female three bands (undigested CHD-1W and digested CHD-1Z). Applying this RFLP method on DNA derived from blood, 9 of the 24 petrels were found to be male, while 15 were females. The same results were obtained using feathers as the source of DNA. To our knowledge, this is the first report of molecular method for sexing this species. The potential of sexing this petrel from feathers is remarkable as it minimizes blood sampling induced stress. This method could be used to reinforce the conservation efforts for this bird, to investigate population sex ratios and to develop new conservation strategies.

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A critique of avianCHD‐based molecular sexing protocols illustrated by a Z‐chromosome polymorphism detected in auklets

Cited by 103 publications

References 11 publications

SEX DETERMINATION OF BALI STARLING (Leucopsar rothschildi) USING MOLECULAR SEXING

SEX DETERMINATION OF BALI STARLING (Leucopsar rothschildi) USING MOLECULAR SEXING

Breeding ecology and bias in offspring sex ratio in little grassbirds (Megalurus gramineus)

Using PCR-RFLP for sexing of the endangered Galápagos petrel (Pterodroma phaeopygia)

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