In computer-mediated communication, emoticons are conventionally rendered in yellow. Previous studies demonstrated that colors evoke certain affective meanings, and face color modulates perceived emotion. We investigated whether color variation affects the recognition of emoticon expressions. Japanese participants were presented with emoticons depicting four basic emotions (Happy, Sad, Angry, Surprised) and a Neutral expression, each rendered in eight colors. Four conditions (E1–E4) were employed in the lab-based experiment; E5, with an additional participant sample, was an online replication of the critical E4. In E1, colored emoticons were categorized in a 5AFC task. In E2–E5, stimulus affective meaning was assessed using visual scales with anchors corresponding to each emotion. The conditions varied in stimulus arrays: E2: light gray emoticons; E3: colored circles; E4 and E5: colored emoticons. The affective meaning of Angry and Sad emoticons was found to be stronger when conferred in warm and cool colors, respectively, the pattern highly consistent between E4 and E5. The affective meaning of colored emoticons is regressed to that of achromatic expression counterparts and decontextualized color. The findings provide evidence that affective congruency of the emoticon expression and the color it is rendered in facilitates recognition of the depicted emotion, augmenting the conveyed emotional message.