2002
DOI: 10.1016/s0304-4017(02)00201-7
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A cross-sectional study of Leishmania spp. infection in clinically healthy dogs with polymerase chain reaction and serology in Greece

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Cited by 117 publications
(116 citation statements)
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“…Moreover, the PCR chosen amplified genomic DNA fragment (PCR gDNA) and not a kinetoplastidic DNA (PCR kDNA) because of the artefacts which can be observed with PCR kDNA and rarely with PCR gDNA and because of the low positive predictive value of the PCR kDNA (Lachaud et al, 2002). The sensitivity of PCR was found higher than that of IFAT (p = 0.004) and in vitro culture (p < 10 -5 ), which is in agreement with results found by other studies (Leontides et al, 2002;Oliva et al, 2006). The low sensitivity of in vitro culture observed in our study is in correlation with what is found in some studies (Berrahal et al, 1996, Fisa et al, 2001.…”
Section: Diagnosis Of Canine Leishmaniasissupporting
confidence: 88%
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“…Moreover, the PCR chosen amplified genomic DNA fragment (PCR gDNA) and not a kinetoplastidic DNA (PCR kDNA) because of the artefacts which can be observed with PCR kDNA and rarely with PCR gDNA and because of the low positive predictive value of the PCR kDNA (Lachaud et al, 2002). The sensitivity of PCR was found higher than that of IFAT (p = 0.004) and in vitro culture (p < 10 -5 ), which is in agreement with results found by other studies (Leontides et al, 2002;Oliva et al, 2006). The low sensitivity of in vitro culture observed in our study is in correlation with what is found in some studies (Berrahal et al, 1996, Fisa et al, 2001.…”
Section: Diagnosis Of Canine Leishmaniasissupporting
confidence: 88%
“…Furthermore, all previous studies have been performed by serological methods. Several studies suggesting that the rate of infection is higher than the figures found by serological investigations (Lachaud et al, 2002;Leontides et al, 2002;Oliva et al, 2006). Several molecular methods had been developed for the detection of Leishmania (Van Eys et al, 1992;Ashford et al, 1995;Spanakos et al, 2002).…”
mentioning
confidence: 92%
“…Additionally, many asymptomatic dogs in endemic areas mount predominantly cellular immunity with a negative to low specific antibody titre and may therefore be missed by serological methods (Baneth et al 2008). For example, of 73 clinically healthy hunting dogs in neighbouring Greece, 12.3 % tested positive by serology, whereas 63 % tested positive by PCR (Leontides et al 2002). The latter authors concluded that most of the dogs residing in areas where leishmaniosis is endemic become infected but often remain seronegative.…”
Section: Discussionmentioning
confidence: 97%
“…For example in areas endemic for canine leishmaniosis (Leishmania infantum) in the Mediterranean basin studies using PCR have confirmed that the prevalence of infection in dogs is much higher than the proportion that actually develops symptomatic disease or even specific antibodies [55,56]. By instance in 73 clinically healthy hunting dogs in Greece, 12.3% tested positive by using serology, whereas 63% tested positive by PCR [57]. These observations suggest that serology and PCR are measuring different aspects of the host-parasite relationship of canine neosporosis.…”
Section: Discussionmentioning
confidence: 99%