A cross-species analysis of the aversiveness of denatonium saccharide and quinine

Davis, Stephen F.; Hoskinson, Kimberly J.; Wilder, Kyle A.; Sander, Julie A.; Larsen, R. Kurt; Knapp, Megan

doi:10.3758/bf03334902

Cited by 2 publications

(2 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In our first pilot experiment, 10 miniature schnauzer dogs were offered a solution of 10μM DB in deionised water in a two-bottle choice test with plain deionised water. In subsequent experiments, a total of 76 dogs of three different breeds (miniature schnauzer (31), Labrador retriever (26), and cocker spaniel (19)), were tested using the same assay, but with a concentration of 100μM DB in deionised water.…”

Section: In Vivo Testing Of Dogs With Denatonium Benzoatementioning

confidence: 99%

See 1 more Smart Citation

Bitter taste sensitivity in domestic dogs (Canis familiaris) and its relevance to bitter deterrents of ingestion

Gibbs

Winnig²,

Riva

et al. 2022

PLoS ONE

View full text Add to dashboard Cite

As the most favoured animal companion of humans, dogs occupy a unique place in society. Understanding the senses of the dog can bring benefits to both the dogs themselves and their owners. In the case of bitter taste, research may provide useful information on sensitivity to, and acceptance of, diets containing bitter tasting materials. It may also help to protect dogs from the accidental ingestion of toxic substances, as in some instances bitter tasting additives are used as deterrents to ingestion. In this study we examined the receptive range of dog bitter taste receptors (Tas2rs). We found that orthologous dog and human receptors do not always share the same receptive ranges using in vitro assays. One bitter chemical often used as a deterrent, denatonium benzoate, is only moderately active against dTas2r4, and is almost completely inactive against other dog Tas2rs, including dTas2r10, a highly sensitive receptor in humans. We substituted amino acids to create chimeric dog-human versions of the Tas2r10 receptor and found the ECL2 region partly determined denatonium sensitivity. We further confirmed the reduced sensitivity of dogs to this compound in vivo. A concentration of 100μM (44.7ppm) denatonium benzoate was effective as a deterrent to dog ingestion in a two-bottle choice test indicating higher concentrations may increase efficacy for dogs. These data can inform the choice and concentration of bitter deterrents added to toxic substances to help reduce the occurrence of accidental dog poisonings.

show abstract

Section: In Vivo Testing Of Dogs With Denatonium Benzoatementioning

confidence: 99%

“…Accidental exposure of pet dogs to such products is quite common [ 30 ]. Some mammals are less sensitive to commonly used bitter tastants than humans [ 31 , 32 ], but the molecular basis for these differences are unknown.…”

Section: Introductionmentioning

confidence: 99%

Bitter taste sensitivity in domestic dogs (Canis familiaris) and its relevance to bitter deterrents of ingestion

Gibbs

Winnig²,

Riva

et al. 2022

PLoS ONE

View full text Add to dashboard Cite

show abstract

Aversiveness of Denatonium Saccharide and Quinine in Grasshopper Mice Onychomys Leucogaster

1989

View full text Add to dashboard Cite

The responses toward denatonium saccharide and quinine by three rodent species differ from those exhibited by humans. This study measured the relative aversiveness of these two compounds in grasshopper mice (Onychomys leucogaster) to determine if this species responded similarly to or differently from these other rodents. In a choice test, the mice clearly preferred quinine over denatonium saccharide at concentrations of 1/10,000 and 1/50,000, but only marginally preferred quinine at a concentration of 1/1000. When denatonium or quinine was paired with toxicosis, both suppressed drinking at concentrations from 1/10,000 to 1/50,000, but only denatonium suppressed drinking at 1/100,000. Neither compound suppressed drinking at 1 part in million. Grasshopper mice perceived denatonium saccharide as more aversive than quinine in both experiments. Such a response resembled the behavior of humans more than that of the other three rodents previously tested.

show abstract

A cross-species analysis of the aversiveness of denatonium saccharide and quinine

Cited by 2 publications

References 4 publications

Bitter taste sensitivity in domestic dogs (Canis familiaris) and its relevance to bitter deterrents of ingestion

Bitter taste sensitivity in domestic dogs (Canis familiaris) and its relevance to bitter deterrents of ingestion

Aversiveness of Denatonium Saccharide and Quinine in Grasshopper Mice Onychomys Leucogaster

Contact Info

Product

Resources

About