A crosslinked colloidal network of peptide/nucleic base amphiphiles for targeted cancer cell encapsulation

Zhou, Yanzi; Qiu, Ping; Yao, Defan; Song, Yanyan; Zhu, Yuedong; Pan, Haiting; Wu, Junchen; Zhang, Junji

doi:10.1039/d1sc02995a

Cited by 7 publications

(3 citation statements)

References 39 publications

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“…First, a model OBOC library was prepared. The α v β 3 targeting peptide RGD was the most commonly used molecule to target MDA-MB-231 tumors. − We conjugated targeting peptide RGDGRGD on beads (RGD beads) as targeting beads and peptide GAGAGAG (GAG beads) as nontargeting beads. To differentiate these two kinds of beads, we labeled RGD beads with Rhodamine B (RhB) and GAG beads with Cyanine 5 (Cy5).…”

Section: Resultsmentioning

confidence: 99%

Localized Instillation Enables In Vivo Screening of Targeting Peptides Using One-Bead One-Compound Technology

et al. 2023

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The One-Bead One-Compound (OBOC) library screening is an efficient technique for identifying targeting peptides. However, due to the relatively large bead size, it is challenging for the OBOC method to be applied for in vivo screening. Herein, we report an in vivo Localized Instillation Beads library (LIB) screening method to discover targeting peptides with the OBOC technique. Inspired by localized instillation, we constructed a cavity inside of a transplanted tumor of a mouse. Then, the OBOC heptapeptide library was injected and incubated inside the tumor cavity. After an efficient elution process, the retained beads were gathered, from which three MDA-MB-231 tumor-targeting heptapeptides were discovered. It was verified that the best peptide had 1.9-fold higher tumor accumulation than the commonly used targeting peptide RGD in vivo. Finally, two targeting proteins were discovered as potential targets of our targeting peptide to the MDA-MB-231 tumor. The in vivo LIB screening method expands the scope of OBOC peptide screening applications to discover targeting peptides in vivo feasibly and reliably.

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Section: Resultsmentioning

confidence: 99%

Localized Instillation Enables In Vivo Screening of Targeting Peptides Using One-Bead One-Compound Technology

et al. 2023

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“…Supramolecular hydrogel, consisting of water and a supragelator, has recently attracted increased attention. As an emerging material, supramolecular hydrogel has a variety of applications, including drug delivery, [ 1‐4 ] tissue engineering, [ 5‐6 ] analyte detection, [ 7‐8 ] wound healing, [ 9‐12 ] serving as an immunoadjuvant, [ 13‐15 ] and cancer inhibition. [ 16‐20 ] Guanosine, one of the natural small molecules, is an extensively explored small molecule for supramolecular hydrogel because of its simplicity and easy preparation.…”

Section: Background and Originality Contentmentioning

confidence: 99%

Hierarchical Self‐assembly of G‐Quadruplexes Based Hydrogel Consisting of Guanine and Peptide Epitope^†

et al. 2023

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Guanosine-based hydrogels have attracted considerable attention because of their simplicity and easy preparation. However, the sugar moiety limits its further applications because of the necessity of sugar in the hydrogel formation. This work reports a G-quadruplexes-based hydrogel consisting of guanine and peptide epitope to form a supramolecular hydrogel in the presence of metal cations. Using the metal ion-responsive peptide epitope from the ion channel to replace sugar motif at N9 position of guanosine results in a novel nucleopeptide. The results show that the gelation time, the diameter of nanofibers, the anisotropic property, and the mechanical property of the hydrogel can be simply controlled using metal cations. The magnesium and calcium ions direct the alignment of nanofibers to form anisotropic nano-bundles. The mechanistic studies indicate the formation of G-quadruplexes in the hydrogel. Compared to the storage modulus of nucleopeptide without the metal cation, adding zinc ions results in an over three-order increase in mechanical properties. Cytotoxicity experiment indicates the good biocompatibility of our hydrogel. Moreover, we demonstrate that the guanine-capped peptide could release STING agonist in a controlled manner. This work illustrates a simple way to modulate the property of the nucleopeptide hydrogel to develop soft materials.

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“…First, on the tumor cell membrane, diverse proteins and receptors are upregulated, such as human epidermal growth factor receptor (HER), integrin, [17] and programmed‐death ligand 1 [18] . Utilizing specific peptides that are recognized by receptors, precursors are transformed into nanofibers.…”

Section: Nanofiber Formation In Organellesmentioning

confidence: 99%

Nanofibers in Organelles: From Structure Design to Biomedical Applications

Hu,

Lin,

Zhang

et al. 2023

Angew Chem Int Ed

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Nanofibers are one of the most important morphologies of molecular self‐assemblies, the formation of which relies on the diverse intermolecular interactions of fibrous units. In the past decade, rapid advances have been made in the biomedical application of nanofibers, such as bioimaging and tumor treatment. An important topic to be focused on is not only the nanofiber formation mechanism but also where it forms, because different destinations could have different influences on cells and its formation could be triggered by unique stimuli in organelles. It is therefore necessary and timely to summarize the nanofibers assembled in organelles. This minireview discusses the formation mechanism, triggering strategies, and biomedical applications of nanofibers, which may facilitate the rational design of nanofibers, improve our understanding of the relationship between nanofiber properties and organelle characteristics, allow a comprehensive recognition of organelles affected by materials, and enhance the therapeutic efficiency of nanofibers.

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A crosslinked colloidal network of peptide/nucleic base amphiphiles for targeted cancer cell encapsulation

Abstract: A cross-linking of peptide–nucleic base amphiphiles leads to a dense colloidal network that can perform targeted cancer cell encapsulation in situ.

Cited by 7 publications

References 39 publications

Localized Instillation Enables In Vivo Screening of Targeting Peptides Using One-Bead One-Compound Technology

Localized Instillation Enables In Vivo Screening of Targeting Peptides Using One-Bead One-Compound Technology

Hierarchical Self‐assembly of G‐Quadruplexes Based Hydrogel Consisting of Guanine and Peptide Epitope^†

Nanofibers in Organelles: From Structure Design to Biomedical Applications

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A crosslinked colloidal network of peptide/nucleic base amphiphiles for targeted cancer cell encapsulation

Abstract: A cross-linking of peptide–nucleic base amphiphiles leads to a dense colloidal network that can perform targeted cancer cell encapsulation in situ.

Cited by 7 publications

References 39 publications

Localized Instillation Enables In Vivo Screening of Targeting Peptides Using One-Bead One-Compound Technology

Localized Instillation Enables In Vivo Screening of Targeting Peptides Using One-Bead One-Compound Technology

Hierarchical Self‐assembly of G‐Quadruplexes Based Hydrogel Consisting of Guanine and Peptide Epitope†

Nanofibers in Organelles: From Structure Design to Biomedical Applications

Contact Info

Product

Resources

About

Hierarchical Self‐assembly of G‐Quadruplexes Based Hydrogel Consisting of Guanine and Peptide Epitope^†