1953
DOI: 10.1016/0014-4827(53)90089-5
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A culture chamber for the continuous biochemical and morphological study of living cells in tissue culture

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Cited by 38 publications
(12 citation statements)
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“…The use of a water circulating system for temperature control was preferred to an electrical heating element (see, for example, Lowndes & Hallet, 1986) because the former also provides the opportunity to cool the central well to below room temperature. Similar microperfusion chambers tend to be constructed such that the glass coverslips are sealed in position with wax or petrolatum (Pomerat, 1951;Christiansen et al, 1953;Toy & Bardawil, 1958;Cruikshank et al, 1959). Others use a sealing similar to that used here (Buchsbaum & Kuntz, 1954;Richter & Woodward, 1955;Sykes & Moore, 1959;Thomas & Cramer, 1966;White, 1966;Poyton & Branton, 1970;Dvorak & Stotler, 1971), but none of these allows the temperature of the cell compartment to be controlled.…”
Section: Discussionmentioning
confidence: 99%
“…The use of a water circulating system for temperature control was preferred to an electrical heating element (see, for example, Lowndes & Hallet, 1986) because the former also provides the opportunity to cool the central well to below room temperature. Similar microperfusion chambers tend to be constructed such that the glass coverslips are sealed in position with wax or petrolatum (Pomerat, 1951;Christiansen et al, 1953;Toy & Bardawil, 1958;Cruikshank et al, 1959). Others use a sealing similar to that used here (Buchsbaum & Kuntz, 1954;Richter & Woodward, 1955;Sykes & Moore, 1959;Thomas & Cramer, 1966;White, 1966;Poyton & Branton, 1970;Dvorak & Stotler, 1971), but none of these allows the temperature of the cell compartment to be controlled.…”
Section: Discussionmentioning
confidence: 99%
“…Since the early 1950s, a variety of culture systems have been designed in parallel with improvements in microscopy and cell culture techniques (3). However, most of the available designs have been developed to meet a limited number of criteria (46).…”
Section: Introductionmentioning
confidence: 99%
“…A number of different types of container for growing cells under visual observation have been described (for example : Carrel, 1923;Hu et al, 1951;Christiansen et al, 1953;Earle & Highhouse, 1954;Rose, 1954;Dvorak & Stotler, 1971). These generally fall into two mutually exclusive classes : those designed to obtain maximum resolution from the light microscope, and those intended to simulate the usual cell culture conditions.…”
Section: Introductionmentioning
confidence: 99%