A culture vessel for amniotic fluid cells allowing faster preparation of chromosome slides

Abstract

A new culture vessel for amniotic fluid culture is presented (flaskette). It consists of a microscope slide, on top of which a culture chamber is mounted. Amniotic fluid cell cultures using in situ technique in the flaskette were compared to subcultured samples in ordinary (Falcon) tissue culture bottles. Working time was reduced by using this new culture vessel because of a very simple harvest procedure, allowing simultaneous harvest of 15 samples. The interval between amniocentesis and harvest was shorter for the in situ technique than for the subcultivation technique. The frequency of aneuploidy in individual metaphases was higher with the subcultivation technique, while there was no difference in the frequency of structural anomalies.

Cytogenetic analysis of 2928 CVS samples and 1075 amniocenteses from randomized studies

“…The short-term, method was routinely used as a back-up in cases of suspected MCC in cultures. Amniotic fluid cells were subcultivated by a modification of the method described by Tabor et al (1984). G-banding was achieved by treatment with trypsin/Leishman stain.…”

Rapid prenatal diagnosis of chromosome aneuploidies by interphase fluorescence in situ hybridization: a one‐year clinical experience with high‐risk and urgent fetal and postnatal samples

“…4 See Tables IV and V. 5 Karyotypes of the two mosaics: 47,XX,π8/46,XX and 47,XY,π10/46,XY. 6 Actually only 41 abnormalities were detected by conventional cytogenetics. One further abnormality was detected due to FISH analysis results.…”

PRENATAL DETECTION OF CHROMOSOME ANEUPLOIDIES BY FLUORESCENCE IN SITU HYBRIDIZATION: EXPERIENCE WITH 2000 UNCULTURED AMNIOTIC FLUID SAMPLES IN A PROSPECTIVE PRECLINICAL TRIAL

“…One of these cultures was subcultivated and the cytogenetic analyses of G-banded chromosomes were performed by the in situ technique (Tabor et al, 1984). At least ten metaphase cells were analysed.…”