2023
DOI: 10.3389/fgene.2023.1159109
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A current and future perspective on T cell receptor repertoire profiling

Abstract: T cell receptors (TCR) play a vital role in the immune system’s ability to recognize and respond to foreign antigens, relying on the highly polymorphic rearrangement of TCR genes. The recognition of autologous peptides by adaptive immunity may lead to the development and progression of autoimmune diseases. Understanding the specific TCR involved in this process can provide insights into the autoimmune process. RNA-seq (RNA sequencing) is a valuable tool for studying TCR repertoires by providing a comprehensive… Show more

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Cited by 7 publications
(5 citation statements)
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“…The innovation and evolution of next generation sequencing methodologies facilitate the decoding and deep analysis of complex scientific questions. The TCRβ sequencing enables the identification and quantification of antigen-specific T-cell receptors as well as their clonotypes [ 28 , 29 ]. This approach is accurate and can eliminate false negative results of other different methods, for example ELISA, as it is based on deep sequencing with high sensitivity and specificity [ 12 , 30 ].…”
Section: Discussionmentioning
confidence: 99%
“…The innovation and evolution of next generation sequencing methodologies facilitate the decoding and deep analysis of complex scientific questions. The TCRβ sequencing enables the identification and quantification of antigen-specific T-cell receptors as well as their clonotypes [ 28 , 29 ]. This approach is accurate and can eliminate false negative results of other different methods, for example ELISA, as it is based on deep sequencing with high sensitivity and specificity [ 12 , 30 ].…”
Section: Discussionmentioning
confidence: 99%
“…5 T-cell receptor (TCR) repertoires refer to the collection of different TCRs found in T cells, and each T-cell typically expresses a specific type of TCR that determines its ability to recognize and respond to specific antigens. 6 The diversity of these TCRs is crucial for the immune system, and specific TCR repertoires may be associated with particular allergic subtypes or phenotypes. 7,8 However, the differences in TCR repertoires in the development and progression of asthma and AD have not been fully explored.…”
Section: Introductionmentioning
confidence: 99%
“…T cells are typically involved in recognizing antigens, and the combination of different Variable (V), Diversity (D), and Joining (J) gene segments results in the unique antigen‐binding sites on T‐cell receptors 5 . T‐cell receptor (TCR) repertoires refer to the collection of different TCRs found in T cells, and each T‐cell typically expresses a specific type of TCR that determines its ability to recognize and respond to specific antigens 6 . The diversity of these TCRs is crucial for the immune system, and specific TCR repertoires may be associated with particular allergic subtypes or phenotypes 7,8 .…”
Section: Introductionmentioning
confidence: 99%
“…CDR3 regions exhibit remarkably high diversity through the V(D)J recombination mechanism, allowing them to adapt to a wide range of existing and potential antigens [ 2 ]. The potential number of TCR variants that can be generated is estimated to reach 10 18 or more [ 3 ]. The importance of TCR diversity in disease monitoring, autoimmune diseases and anticancer immunity has driven research on the rules governing CDR3 epitope binding.…”
Section: Introductionmentioning
confidence: 99%