A curved‐field reflectron for improved energy focusing of product ions in time‐of‐flight mass spectrometry

Cornish, Timothy J.; Cotter, Robert J.

doi:10.1002/rcm.1290071114

Cited by 110 publications

(56 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Further, tandem time-of-flight mass spectrometry (TOF/TOF) is a technique in which two time-of-flight mass spectrometers are arranged in tandem and used consecutively [1][2][3][4][5][6][7][8][9][10][11][12][13][14]. Precursor ions are selected by the first time-of-flight mass spectrometer (TOF1), and the fragment ions derived from them are mass analyzed by the second time-of-flight mass spectrometer (TOF2).…”

Section: Introductionmentioning

confidence: 99%

Tandem Time-of-Flight Mass Spectrometer with High Precursor Ion Selectivity Employing Spiral Ion Trajectory and Improved Offset Parabolic Reflectron

Satoh

Sato

Kubo

et al. 2011

J. Am. Soc. Mass Spectrom.

View full text Add to dashboard Cite

In this study, we have developed a tandem time-of-flight mass spectrometry (TOF/TOF) technique involving the use of a matrix-assisted laser desorption/ionization ion source that exhibits high precursor ion selectivity. An ion optical system with a 17 m spiral ion trajectory was used in the first time-of-flight mass spectrometer. High precursor ion selectivity was achieved by realizing a 15 m flight path, which is considerably longer than that of the conventional MALDI-TOF/TOF before the precursor ion selection by an ion gate; monoisotopic ions could be selected properly up to m/z 2500. Furthermore, the first time-of-flight mass spectrometer was composed of electrostatic sectors and could eliminate post-source decay (PSD) ions. Precursor ions with 20 keV kinetic energy were selected and injected into a collision cell, leading to the generation of fragment ions by high-energy collision-induced dissociation (HE-CID). The optimized second time-of-flight mass spectrometer included a post-acceleration region and an offset parabolic reflectron to record product ion spectra in the entire mass range. Our system could generate a simple HE-CID product ion spectrum because each fragment pathway could be observed as a single peak by the selection of monoisotopic ions of all precursor ions and HE-CID fragment pathways could be predominantly observed by the PSD ion elimination.

show abstract

Section: Introductionmentioning

confidence: 99%

Tandem Time-of-Flight Mass Spectrometer with High Precursor Ion Selectivity Employing Spiral Ion Trajectory and Improved Offset Parabolic Reflectron

Satoh

Sato

Kubo

et al. 2011

J. Am. Soc. Mass Spectrom.

View full text Add to dashboard Cite

show abstract

“…This method was then applied to samples isolated from baker's yeast which require ultimate sensitivity. The noteworthy property of this type of reflector [19][20][21][22] is that it brings into simultaneous focus product ions created over a broad m/z range and obviates the need for stepping the reflectron voltage to reconstruct the PSD spectrum.…”

Section: Introductionmentioning

confidence: 99%

Negative Ion Ultraviolet Matrix-Assisted Laser Desorption Ionization Mass Spectrometry and Post Source Decay of Glycosyl Esters of Nucleoside Pyrophosphates

Heinrich

Schäffer

Messner

et al. 2008

Eur J Mass Spectrom (Chichester)

View full text Add to dashboard Cite

Six different glycosyl esters of nucleoside pyrophosphates (monosaccharide nucleotides) were analyzed by means of matrix-assisted laser desorption/ionisation reflectron time-of-flight mass spectrometry (MALDI/RToF MS) in the negative ion mode. Several matrices were evaluated and 3-hydroxypicolinic acid as well as α-cyano-4-hydroxycinnamic acid (CHCA) turned out to be the matrices of choice applying the thin layer technique to obtain maximum sensitivity for deprotonated molecular ion detection and maximal fragmentation particular with CHCA. The determination of the molecular mass with a mass accuracy below 0.1% was feasible with sample amounts in the lower femtomole range applying a MALDI desk-top mass spectrometer. A further important refinement of this technique was the use of post source decay (PSD) fragment ion analysis with a curved field reflector (which means no stepping of the reflector voltage). Detailed structural information of the six selected monosaccharide nucleotides could be obtained with PSD and differences in the fragmentation pattern were used to distinguish them. This method (based on molecular mass and PSD fragment ion analysis) has been applied to verify the presence of a glycosyl ester of nucleoside pyrophosphate in samples from Saccharomyces cerevisiae. KeywordsMALDI; post source decay; mass spectrometry; carbohydrate biosynthesis; glycosyl ester of nucleoside pyrophosphate; negative ions; ToF

show abstract

“…Thus, the curved-field reflectron (CFR) was developed to enable us to utilize the full kinetic energy, resulting from the initial ion acceleration, as the collision energy. 4) The CFR, while developed for the tandem instrument, was also very e#ective for obtaining postsource decay (PSD) product ion mass spectra on a single reflectron instrument. 5), 6) PSD was introduced by Spengler et al 7) for single mass analyzer instruments with a reflectron, but required changing the reflectron voltage to focus separately di#erent regions of the product ion mass spectrum.…”

Section: Introductionmentioning

confidence: 99%

Tandem Time-of-Flight (TOF/TOF) Mass Spectrometry and Proteomics

Cotter

Iltchenko

Wang

et al. 2005

J. Mass Spectrom. Soc. Jpn.

Self Cite

View full text Add to dashboard Cite

ῌ 7 ῌ photodissociation. In our case, however, reduction of the kinetic energy E 1 also reduces the available collision energy.Thus, the curved-field reflectron (CFR) was developed to enable us to utilize the full kinetic energy, resulting from the initial ion acceleration, as the collision energy. 4)The CFR, while developed for the tandem instrument, was also very e#ective for obtaining postsource decay (PSD) product ion mass spectra on a single reflectron instrument. 5), 6)PSD was introduced by Spengler et al. 7) for single mass analyzer instruments with a reflectron, but required changing the reflectron voltage to focus separately di#erent regions of the product ion mass spectrum. The CFR, however, enabled recording of the entire product ion mass range at a single, fixed voltage. Fig. 2. An approach to the design of a tandem TOF mass spectrometer described in ref. 8, in which precursor ions are decelerated prior to entering the collision chamber, and the product ions are then extracted and reaccelerated from a pulsed "second source" before entering the reflectron. Fig. 3. An approach to the design of a tandem mass spectrometer described in ref. 9, in which the product ions with velocities equal to that of their precursor enter a "lift cell" prior to mass analysis.R. J. Cotter, et al.ῌ 8 ῌ Results and Discussion2.1 Tandem mass spectrometers using pulsed extraction and a single reflectron One of the disadvantages of the two reflectron instrument was that considerable post-source decay occurred before the ions entered the first reflectron. The resulting product ions do not arrive at the collision chamber and mass-selector at the same time as their precursor, and so are gated out from the spectrum. Thus, a considerably more sensible approach would be to use a simple, linear TOF as the first mass analyzer, using the fact that precursors and their products will maintain the same velocity and reach the mass-selector at the same time. The combination of a pulsed, linear TOF as a first mass analyzer and a reflectron second mass analyzer is the approach generally used in current tandem TOF instruments, though in some cases deceleration of the ions prior to reaching the collision chamber loses the advantage of recording all mass-selected Fig. 4. An approach to the design of a tandem mass spectrometer described in ref. 10 that does not require deceleration or reacceleration, but focuses product ions in a curved-field reflectron.Fig. 5. Collision-induced dissociation, tandem mass spectra of buckminsterfullerene (C60) at di#erent pressures of helium collision gas.Tandem Time-of-Flight (TOF/TOF) Mass Spectrometry and Proteomics ῌ 9 ῌ post source product ions. For example, in the instrument shown in Fig. 2a and described by Medzihradszky et al. 8) ions formed in the source are accelerated to energies of 20 keV, massselected by a timed ion gate, and then decelerated to 1῍ 2 keV before entering the collision chamber. After the ions are passed through the collision chamber, the remaining precursor and the product ions are rea...

show abstract

A curved‐field reflectron for improved energy focusing of product ions in time‐of‐flight mass spectrometry

Cited by 110 publications

References 6 publications

Tandem Time-of-Flight Mass Spectrometer with High Precursor Ion Selectivity Employing Spiral Ion Trajectory and Improved Offset Parabolic Reflectron

Tandem Time-of-Flight Mass Spectrometer with High Precursor Ion Selectivity Employing Spiral Ion Trajectory and Improved Offset Parabolic Reflectron

Negative Ion Ultraviolet Matrix-Assisted Laser Desorption Ionization Mass Spectrometry and Post Source Decay of Glycosyl Esters of Nucleoside Pyrophosphates

Tandem Time-of-Flight (TOF/TOF) Mass Spectrometry and Proteomics

Contact Info

Product

Resources

About