A cyanobacterial β-actin-like protein, responsible for lichenized Nostoc sp. motility towards a fungal lectin

Díaz, Eva María; Vicente‐Manzanares, Miguel; Legaz, Marı́a-Estrella; Vicente, Carlos

doi:10.1007/s11738-015-2007-4

Cited by 5 publications

(3 citation statements)

References 58 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, this has not been reported in a lichen cyanobiont. Our data are further supported by the suppression of chemotaxis produced by cytoskeleton inhibitors (Díaz et al, 2015). Together, this suggests the existence of an actinlike apparatus that supports chemotactic swimming, similar to that observed in neutrophils (Ehrengruber et al, 1996).…”

Section: Discussionsupporting

confidence: 86%

An actomyosin-like cytoskeleton in the cyanobiont (Nosctoc sp.) of Peltigera canina

Díaz

Ampè

Troys

et al. 2016

Phytochemistry Letters

Self Cite

View full text Add to dashboard Cite

Section: Discussionsupporting

confidence: 86%

An actomyosin-like cytoskeleton in the cyanobiont (Nosctoc sp.) of Peltigera canina

Díaz

Ampè

Troys

et al. 2016

Phytochemistry Letters

Self Cite

View full text Add to dashboard Cite

“…The lectin recognition and signalling mechanism summarised for chlorolichens in Figure 2 can be true of all or some cyanolichens (Sacristán et al, 2007;Vivas et al, 2010;Díaz et al, 2016a). Diaz et al (2015), Diaz et al (2016b) showed that actin-and myosin-like proteins produced by the cyanobacterial photobiont Nostoc of the lichen Peltigera canina is involved in the chemotactic movement of photobiont cells towards the lectin of the mycobiont. The process also involves a contractile protein and ATPase of photobiont, which creates a series of contraction-relaxation steps that result in photobiont # Ribitol in these pre-contact experiments (mycobiont and photobiont separated by a membrane) was added in concentrations of 0.05, 0.8, and 2.0%w/v to the culture media (water agar and malt yeast agar).…”

Section: Cyanolichens (Tablementioning

confidence: 99%

Towards a Systems Biology Approach to Understanding the Lichen Symbiosis: Opportunities and Challenges of Implementing Network Modelling

et al. 2021

View full text Add to dashboard Cite

Lichen associations, a classic model for successful and sustainable interactions between micro-organisms, have been studied for many years. However, there are significant gaps in our understanding about how the lichen symbiosis operates at the molecular level. This review addresses opportunities for expanding current knowledge on signalling and metabolic interplays in the lichen symbiosis using the tools and approaches of systems biology, particularly network modelling. The largely unexplored nature of symbiont recognition and metabolic interdependency in lichens could benefit from applying a holistic approach to understand underlying molecular mechanisms and processes. Together with ‘omics’ approaches, the application of signalling and metabolic network modelling could provide predictive means to gain insights into lichen signalling and metabolic pathways. First, we review the major signalling and recognition modalities in the lichen symbioses studied to date, and then describe how modelling signalling networks could enhance our understanding of symbiont recognition, particularly leveraging omics techniques. Next, we highlight the current state of knowledge on lichen metabolism. We also discuss metabolic network modelling as a tool to simulate flux distribution in lichen metabolic pathways and to analyse the co-dependence between symbionts. This is especially important given the growing number of lichen genomes now available and improved computational tools for reconstructing such models. We highlight the benefits and possible bottlenecks for implementing different types of network models as applied to the study of lichens.

show abstract

“…This means that, in the presence of compatible fungus, the binding of the lectin to its specific cell wall receptor would activate the signaling pathway that involves cytoskeleton reorganization. It must take place probably by means of GTPase activity, since the inhibition of chemotaxis produced by the combined action of phalloidin and blebbistatin is largely reversed by GTP and its analogs, GTP(γ)S and GDP(β)S, as well as by cyclic AMP [48]. On the contrary, when it is a noncompatible interaction, lectin penetrates into the cell, promoting putrescine synthesis.…”

Section: Cytoskeleton Reorganization In Nostoc Cells In Response To Tmentioning

confidence: 99%

Role of the Cytoskeletal Actomyosin Complex in the Motility of Cyanobacteria and Fungal Spores

Sánchez-Elordi¹,

Díaz²,

Vicente³

et al. 2020

Parasitology and Microbiology Research

Self Cite

View full text Add to dashboard Cite

This study demonstrates the involvement of the cytoskeleton in the movement of cyanobacteria and fungal spores to their hosts to establish a state of symbiosis or pathogenicity. The term symbiosis sensu lato is referred not only to commensalism and mutualism but also to the parasitic aberrations. The establishment of association implies that the endohabitant can move on a wet surface until finding an entry point in the exohabitant surface. In aqueous media, the exohabitant secretes glycoproteins that form a chemoattraction gradient for the invading cells. In lichens, the gradient consists of fungal lectins whose function is to recognize a compatible green alga or cyanobacterium. In the case of pathogens, the secreted proteins usually are a mixture that includes false quorum and chemoattractant signals, and cell wall digestive enzymes. The results indicate that fungal lectins and defense proteins bind to specific cell wall receptors for signaling the activation of cytoskeleton, causing successive cycles of cell contraction-relaxation that permits the migration of the endohabitant. In this study, different biochemical and microscopy techniques have been used. The mechanisms through which the cytoskeleton carries out these cycles of cell contractionrelaxation are described, being this a remarkable advance compared to previous results.

show abstract

A cyanobacterial β-actin-like protein, responsible for lichenized Nostoc sp. motility towards a fungal lectin

Cited by 5 publications

References 58 publications

An actomyosin-like cytoskeleton in the cyanobiont (Nosctoc sp.) of Peltigera canina

An actomyosin-like cytoskeleton in the cyanobiont (Nosctoc sp.) of Peltigera canina

Towards a Systems Biology Approach to Understanding the Lichen Symbiosis: Opportunities and Challenges of Implementing Network Modelling

Role of the Cytoskeletal Actomyosin Complex in the Motility of Cyanobacteria and Fungal Spores

Contact Info

Product

Resources

About