A cyclic nucleotide‐gated channel is necessary for optimum fertility in high‐calcium environments

Chaiwongsar, Suraphon; Strohm, Allison K.; Roe, Joshua; Godiwalla, Roxana Y.; Chan, Catherine W. M.

doi:10.1111/j.1469-8137.2009.02833.x

Cited by 23 publications

(16 citation statements)

References 39 publications

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“…This level of lethality is 2-to 3-fold higher than a wild-type control. An analogous CaCl 2 hypersensitivity has been reported for two other cngc mutant phenotypes (Chaiwongsar et al, 2009;Urquhart et al, 2011). For example, the growth reduction phenotype associated with a knockout of cngc2 in Arabidopsis was further decreased by approximately 2-fold by supplementing soil with 10 mM CaCl 2 .…”

Section: Germination Of Cngc16 Pollen Grains Is Highly Sensitive To Ementioning

confidence: 84%

A Cyclic Nucleotide-Gated Channel (CNGC16) in Pollen Is Critical for Stress Tolerance in Pollen Reproductive Development

et al. 2012

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Cyclic nucleotide-gated channels (CNGCs) have been implicated in diverse aspects of plant growth and development, including responses to biotic and abiotic stress, as well as pollen tube growth and fertility. Here, genetic evidence identifies CNGC16 in Arabidopsis (Arabidopsis thaliana) as critical for pollen fertility under conditions of heat stress and drought. Two independent transfer DNA disruptions of cngc16 resulted in a greater than 10-fold stress-dependent reduction in pollen fitness and seed set. This phenotype was fully rescued through pollen expression of a CNGC16 transgene, indicating that cngc16-1 and 16-2 were both loss-of-function null alleles. The most stress-sensitive period for cngc16 pollen was during germination and the initiation of pollen tube tip growth. Pollen viability assays indicate that mutant pollen are also hypersensitive to external calcium chloride, a phenomenon analogous to calcium chloride hypersensitivities observed in other cngc mutants. A heat stress was found to increase concentrations of 39,59-cyclic guanyl monophosphate in both pollen and leaves, as detected using an antibody-binding assay. A quantitative PCR analysis indicates that cngc16 mutant pollen have attenuated expression of several heat-stress response genes, including two heat shock transcription factor genes, HsfA2 and HsfB1. Together, these results provide evidence for a heat stress response pathway in pollen that connects a cyclic nucleotide signal, a Ca 2+ -permeable ion channel, and a signaling network that activates a downstream transcriptional heat shock response.

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Section: Germination Of Cngc16 Pollen Grains Is Highly Sensitive To Ementioning

confidence: 84%

A Cyclic Nucleotide-Gated Channel (CNGC16) in Pollen Is Critical for Stress Tolerance in Pollen Reproductive Development

et al. 2012

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“…Many studies have explored the role of CNGC2 (CYCLIC NUCLEOTIDE GATED CHANNEL2) in regulation of Ca 2+ fluxes across the plasma membrane and its contribution to signaling in the context of immunity [53,54], senescence [55], heat stress [56], and pollen growth [57]. Null mutation of CNGC2 was first isolated as defense no death1 ( dnd1 ), a mutant which exhibits a lesion mimic phenotype which is dependent on growth conditions [58], increased accumulation of SA and constitutive defense activation [59], and altered Ca 2+ transport [60].…”

Section: Introductionmentioning

confidence: 99%

Salicylic acid signaling inhibits apoplastic reactive oxygen species signaling

Brosché

2014

BMC Plant Biol

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BackgroundReactive oxygen species (ROS) are used by plants as signaling molecules during stress and development. Given the amount of possible challenges a plant face from their environment, plants need to activate and prioritize between potentially conflicting defense signaling pathways. Until recently, most studies on signal interactions have focused on phytohormone interaction, such as the antagonistic relationship between salicylic acid (SA)-jasmonic acid and cytokinin-auxin.ResultsIn this study, we report an antagonistic interaction between SA signaling and apoplastic ROS signaling. Treatment with ozone (O3) leads to a ROS burst in the apoplast and induces extensive changes in gene expression and elevation of defense hormones. However, Arabidopsis thaliana dnd1 (defense no death1) exhibited an attenuated response to O3. In addition, the dnd1 mutant displayed constitutive expression of defense genes and spontaneous cell death. To determine the exact process which blocks the apoplastic ROS signaling, double and triple mutants involved in various signaling pathway were generated in dnd1 background. Simultaneous elimination of SA-dependent and SA-independent signaling components from dnd1 restored its responsiveness to O3. Conversely, pre-treatment of plants with SA or using mutants that constitutively activate SA signaling led to an attenuation of changes in gene expression elicited by O3.ConclusionsBased upon these findings, we conclude that plants are able to prioritize the response between ROS and SA via an antagonistic action of SA and SA signaling on apoplastic ROS signaling.

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“…Furthermore, gene expression profiling of cngc2-2 showed strong similarity to the expression pattern of wild-type plants grown at elevated Ca 2+ levels, indicating abnormal Ca 2+ sensing in cngc2 (Chan et al , 2008). Adult cngc2 mutants displayed decreased size and fertility when grown on Ca 2+ -supplemented medium (Chan et al , 2003; Chaiwongsar et al , 2009). Pollen tube growth is another developmental process that is driven by Ca 2+ fluxes (Michard et al , 2009), and it has been shown that the loss of AtCNGC18 causes abnormalities in pollen tube growth (Frietsch et al , 2007; Chang et al , 2008).…”

Section: Introductionmentioning

confidence: 99%

The cyclic nucleotide-gated channels AtCNGC11 and 12 are involved in multiple Ca2+-dependent physiological responses and act in a synergistic manner

Urquhart

Chin

Ung

et al. 2011

Journal of Experimental Botany

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Arabidopsis cyclic nucleotide-gated ion channels (AtCNGCs) form a large family consisting of 20 members. These channels have so far been reported to be involved in a diverse range of physiological phenomena. For example, AtCNGC18 was reported to play an important role in pollen tube growth, while AtCNGC2, 4, 11, and 12 were implicated in mediating pathogen defence. To identify additional functions for AtCNGC11 and 12, various physiological aspects were analysed using both AtCNGC11 and 12 single knockout mutants as well as a double mutant. Although AtCNGC11 and 12 can function as K+ and Ca2+ channels in yeast, it was found that the loss of AtCNGC11 and 12 in Arabidopsis caused increased sensitivity to Ca2+ but not K+, indicating a specific function for these genes in Ca2+ signalling in planta. However, they did not show an alteration in Ca2+ accumulation, suggesting that AtCNGC11 and 12 are not involved in general Ca2+ homeostasis but rather in the endogenous movement of Ca2+ and/or Ca2+ signalling. Furthermore, these channels synergistically contribute to the generation of a Ca2+ signal that leads to gravitropic bending. Finally, AtCNGC11 and 12 gene expression was induced during dark-induced senescence and AtCNGC11 and 12 knockout mutants displayed enhanced chlorophyll loss, which was even more pronounced in the double mutant, also indicating synergistic roles in senescence. The findings indicate that (i) some CNGC family members have multiple physiological functions and (ii) some plant CNGCs share the same biological function and work in a synergistic manner.

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A cyclic nucleotide‐gated channel is necessary for optimum fertility in high‐calcium environments

Cited by 23 publications

References 39 publications

A Cyclic Nucleotide-Gated Channel (CNGC16) in Pollen Is Critical for Stress Tolerance in Pollen Reproductive Development

A Cyclic Nucleotide-Gated Channel (CNGC16) in Pollen Is Critical for Stress Tolerance in Pollen Reproductive Development

Salicylic acid signaling inhibits apoplastic reactive oxygen species signaling

The cyclic nucleotide-gated channels AtCNGC11 and 12 are involved in multiple Ca2+-dependent physiological responses and act in a synergistic manner

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