A cytogenetic study of factors affecting sister chromatid differentiation in Vicia faba and Hordeum vulgare.

Gerster, Jean L.; Grant, W. F.

doi:10.1508/cytologia.54.523

Cited by 4 publications

(1 citation statement)

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“…It was confirmed that the SCE method using the incorporation of the 5-bromodeoxyuridine (BrdU) in combination with 2′deoxyuridine (dUrd) and 5-fluoro-2′-deoxyuridine (FdU) increases the sister chromatid exchange yield (Pardo et al, 1987). The application of BrdU can cause a distortion of the chromosome morphology, a lower proportion of metaphase cells per slide, and problems with poor sister chromatid differentiation as a result of an incorrect BrdU concentration (Gerster and Grant, 1989). The elimination of FdU is also not recommended since it has been shown to enhance the uptake of BrdU.…”

Section: Discussionmentioning

confidence: 96%

EdU-Based Step-by-Step Method for the Detection of Sister Chromatid Exchanges for Application in Plant Genotoxicity Assessment

Kwaśniewska

Bara

2020

Front. Plant Sci.

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This study is an example of using 5-ethynyl-2′-deoxyuridine (EdU) for detecting sister chromatid exchanges (SCEs) at chromosomal level. Here we report a detailed protocol for differential labeling sister chromatids in barley (Hordeum vulgare, 2n = 14) cells that is based on the incorporation and simple detection of EdU. The perfect distinguishing of sister chromatids enabled an analysis of the effects of two model agents-maleic acid hydrazide (MH) and gamma rays-on the formation of SCEs. Using this method, we demonstrated the high sensitivity of barley cells to maleic hydrazide, which is expressed as an increased level of SCEs. A gamma ray induced only slightly more SCEs than in the control cells. The possible mechanisms of MH and gamma ray action in respect to distinguishing chromatids using EdU are discussed. Recommendation for SCEs visualization using EdU as an easy and quick method that can be successfully adapted to other plant species and potentially for human genotoxicity studies is presented.

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Section: Discussionmentioning

confidence: 96%

EdU-Based Step-by-Step Method for the Detection of Sister Chromatid Exchanges for Application in Plant Genotoxicity Assessment

Kwaśniewska

Bara

2020

Front. Plant Sci.

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Sister chromatid exchange in human lymphocytes induced by propoxur following plant activation by Vicia faba

Gómez‐Arroyo¹,

Calderón-Segura²,

Villalobos‐Pietrini³

1995

Environ and Mol Mutagen

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Because the carbamate insecticide propoxur induced sister chromatid exchanges (SCE) in Vicia faba but was ineffective in producing SCE in lymphocytes in culture, it was hardly suspected that plant metabolism was involved. Experiments were conducted in which metabolic activation was afforded by Vicia faba roots, and SCE in human lymphocytes in vitro was used to assess cytogenetic damage. Several concentrations of propoxur (250, 500, 1,000, 1,500, and 2,000 ppm) were applied for 4 hr to the roots of Vicia faba. Extracts prepared from these treatments were added to the lymphocyte cultures and a significant increase of SCE frequencies with a concentration-response relationship could be detected. The lymphocyte proliferation kinetics and the proliferation rate index (PRI) were not affected (except in the highest concentration, of 2,000 ppm). This general behavior was in agreement with the presence of an enzymatic system (S10 fraction) in Vicia roots capable of metabolizing or activating the propoxur. With 2,000 ppm, cell necrosis was produced in Vicia; therefore, this extract did not induce SCE in lymphocytes. However, lymphocyte proliferation kinetics were delayed and PRI was significantly decreased. Ethanol, a promutagen activated by this plant, was applied directly to the lymphocyte cultures as a positive control, and the response was negative. On the other hand, the extracts of roots treated with ethanol increased the SCE to more than twice that of the negative control, but the lymphocyte proliferation kinetics and PRI were not affected.

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Induction of sister chromatid exchanges by heavy metal salts in root meristem cells ofAllium cepa L.

Panda¹,

Patra²,

Panda³

1996

Biologia plant.

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A cytogenetic study of factors affecting sister chromatid differentiation in Vicia faba and Hordeum vulgare.

Cited by 4 publications

References 44 publications

EdU-Based Step-by-Step Method for the Detection of Sister Chromatid Exchanges for Application in Plant Genotoxicity Assessment

EdU-Based Step-by-Step Method for the Detection of Sister Chromatid Exchanges for Application in Plant Genotoxicity Assessment

Sister chromatid exchange in human lymphocytes induced by propoxur following plant activation by Vicia faba

Induction of sister chromatid exchanges by heavy metal salts in root meristem cells ofAllium cepa L.

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