A cytotoxic anti HLA‐AB monoclonal antibody which in dilution becomes specific to HLA‐A3 crossreacting group

Mazzilli, Maria Cristina; Cascino, Isabella; Porrini, Sandro Costanzi; Lavaggi, M. V.; Lulli, Patrizia; Morellini, M; Testa, Luana; Gandini, E.

doi:10.1111/j.1399-0039.1984.tb02113.x

Cited by 9 publications

(2 citation statements)

References 11 publications

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“…We tested the dose‐dependent inhibition of [ 3 H]thymidine incorporation in PHA‐activated T‐lymphocytes induced by the following MAbs: 01.65 and W6/32, which are directed to framework determinants expressed on the heavy chain of HLA class I antigens [1, 16]; XI‐20.4, an anti‐HLA‐A and B [17]; R1.30, an anti‐β 2 ‐microglobulin [18]; and as control P3, an anti‐IgGlk obtained from pristane‐primed mice injected with X63.Ag8 murine parental myeloma cells. MAb 01.65 was selected because of the high inhibitory activity and administered at a dose of 12.5 μg/ml.…”

Section: Methodsmentioning

confidence: 99%

Nuclear accumulation of c‐myc mRNA in phytohaemagglutinin‐activated T lymphocytes treated with anti‐HLA class I monoclonal antibody

et al. 1998

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Anti-HLA class I monoclonal antibody 01.65 inhibits the proliferative response of PHA-activated human T lymphocytes from peripheral blood mononuclear cells. The recruitment rate in the cell cycle is slack and the G1 and S phases are prolonged. Among the early events after PHA activation, only the calcium-dependent PKC activity appears to be modified: particulate PKC is completely depleted while cytosolic residual PKC is reduced by 80% after MAb 01.65 treatment. We have carried out in greater detail the study of c-myc gene regulation by MAb 01.65 and the results are as follows: (i) c-myc RNA transcription is normally initiated and finished, suggesting a post-transcriptional regulation of c-myc gene expression; (ii) no alteration in c-myc mRNA stability has been documented; (iii) steady-state levels of c-myc mRNA expression by Northern blot analysis and PCR amplification are decreased in the cytoplasmic compartment, while in the nuclear compartment they appear to be increased. Nuclear accumulation of mature mRNA after MAb 01.65 and PKC inhibitor (H7 and StSp) treatment appears to be the most probable mechanism involved. The possible implications of this are discussed.z 1998 Federation of European Biochemical Societies.

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Section: Methodsmentioning

confidence: 99%

Nuclear accumulation of c‐myc mRNA in phytohaemagglutinin‐activated T lymphocytes treated with anti‐HLA class I monoclonal antibody

et al. 1998

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“…For A3, the alpha, "A", chain is encoded by the HLA-A*03 allele group. Production of monoclonal antibodies to A3 antigen is of basic and clinical importance [10][11][12][13][14]. This antibody is among several antibodies that are used in serologic typing of HLA class I alleles.…”

Section: Introductionmentioning

confidence: 99%

Production of mouse monoclonal antibodies against human HLA-A3 for use in microlymphocytotoxicity technique

Yari

Sareh

Sadri

et al. 2009

HAB

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Monoclonal antibody production using the hybridoma technology is one of the attractive areas of research. The aim of this study was to investigate the production of monoclonal antibodies to human HLA-A3 antigen for use in microlymphocytotoxicity technique. HLA-A3 antigen was purified from human leukocytes and injected to several mice. The spleen-derived cells of immunized animal were fused with P3-X63-Ag8 myeloma cells. Obtained hybridoma cells were grown in HAT medium and the supernatant of the cells were screened for antibodies using ELISA and microlymphocytotoxicity technique. Produced monoclonal antibody from the hybridoma clone, B1F10 showed the specificity for human HLA-A3. Furthermore, in this study, we encountered with clones of immortal hybrid cells with completely different features, they were adherent and non-producers for antibodies. This observation could imply the possible role of the antigen (HLA) and its nature in the expansion of cell types other than B cells in the spleen of mouse during immunization.

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Mapping of A3 and A2 polymorphic determinants on hybrid HLA antigens

et al. 1987

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A cytotoxic anti HLA‐AB monoclonal antibody which in dilution becomes specific to HLA‐A3 crossreacting group

Cited by 9 publications

References 11 publications

Nuclear accumulation of c‐myc mRNA in phytohaemagglutinin‐activated T lymphocytes treated with anti‐HLA class I monoclonal antibody

Nuclear accumulation of c‐myc mRNA in phytohaemagglutinin‐activated T lymphocytes treated with anti‐HLA class I monoclonal antibody

Production of mouse monoclonal antibodies against human HLA-A3 for use in microlymphocytotoxicity technique

Mapping of A3 and A2 polymorphic determinants on hybrid HLA antigens

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