This paper proposes an overall practical stability assessment for a multi-port single-phase solid-state transformer (MS3T) in the electromagnetic timescale. When multiple stable subsystems are combined into one MS3T, the newly formed MS3T has a certain possibility to be unstable. Thus, this paper discusses the stability assessment of the MS3T in detail. First and foremost, the structure of the MS3T and its three stage control strategies are proposed. Furthermore, the stability analysis of each of the MS3T’s subsystems is achieved through the closed loop transfer function of each subsystem, respectively, including an AC-DC front-end side converter, dual active bridge (DAB) with a high-frequency (HF) or medium-frequency (MF) transformer, and back-end side incorporating DC-AC and dc-dc converters. Furthermore, the practical impedance stability criterion in the electromagnetic timescale, which only requires two current sensors and one external high-bandwidth small-signal sinusoidal perturbation current source, is proposed by the Gershgorin theorem and Kirchhoff laws. Finally, the overall stability assessment, based on a modified impedance criterion for the MS3T is investigated. The overall practical stability assessment of the MS3T can be validated through extensive simulation and hardware results.