This study was devoted to investigate the bias due to entrapped air -in the cylindrical tube water -to a web based rain gauge calibrator (WBRGC). This study was carried out in laboratory temperature around 25 °C using experiment method, especially by comparing the mass of the water with and without bubbles. The entrapped air used the space for 1.57 g of 1009.82 g of water if there were no bubbles, so this condition made bias around -0.16 %.This bias was very significant in contributing the uncertainty of WBRGC, where in this case was 3 ml for 966 ml of water (0.31 %). Hence, this bias should be promptly overcome.