A database of protein expression in lung cancer

Oh, Jane M. C.; Brichory, Franck; Puravs, Eric; Kuick, Rork; Wood, Christopher D.; Rouillard, Jean Marie; Tra, John; Kardia, Sharon L.R.; Beer, David G.; Hanash, Samir M.

doi:10.1002/1615-9861(200110)1:10<1303::aid-prot1303>3.0.co;2-2

Cited by 80 publications

(43 citation statements)

References 16 publications

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“…Proteomic research to date has mainly involved proteomic pattern profiling of tissue and body fluids by mass spectrometry with sophisticated bioinformatic tools to identify proteins within the complex proteomic profile that discriminate between normal, benign, or disease states (52,56). Proteomic approaches have been used with some success for the molecular classification of tumors (62) and to develop a discriminatory pattern that distinguishes normal sera from that of ovarian, prostate, breast, lung cancer, and, more recently, cutaneous T-cell lymphoma (56,(63)(64)(65)(66)(67)(68)(69)(70). To a lesser extent, proteomics has been applied to study physiologic or pathologic changes associated with external ''environmental'' exposures.…”

Section: Metabonomicsmentioning

confidence: 99%

Molecular Epidemiology and Biomarkers in Etiologic Cancer Research: The New in Light of the Old

Víneis

Perera

2007

Cancer Epidemiology, Biomarkers &Amp; Prevention

162

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The purpose of this review is to evaluate progress in molecular epidemiology over the past 24 years in cancer etiology and prevention to draw lessons for future research incorporating the new generation of biomarkers. Molecular epidemiology was introduced in the study of cancer in the early 1980s, with the expectation that it would help overcome some major limitations of epidemiology and facilitate cancer prevention. The expectation was that biomarkers would improve exposure assessment, document early changes preceding disease, and identify subgroups in the population with greater susceptibility to cancer, thereby increasing the ability of epidemiologic studies to identify causes and elucidate mechanisms in carcinogenesis. The first generation of biomarkers has indeed contributed to our understanding of risk and susceptibility related largely to genotoxic carcinogens.Consequently, interventions and policy changes have been mounted to reduce risk from several important environmental carcinogens. Several new and promising biomarkers are now becoming available for epidemiologic studies, thanks to the development of highthroughput technologies and theoretical advances in biology. These include toxicogenomics, alterations in gene methylation and gene expression, proteomics, and metabonomics, which allow large-scale studies, including discovery-oriented as well as hypothesis-testing investigations. However, most of these newer biomarkers have not been adequately validated, and their role in the causal paradigm is not clear. There is a need for their systematic validation using principles and criteria established over the past several decades in molecular cancer epidemiology. (Cancer Epidemiol Biomarkers Prev 2007;16(10):1954 -65)

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Section: Metabonomicsmentioning

confidence: 99%

Molecular Epidemiology and Biomarkers in Etiologic Cancer Research: The New in Light of the Old

Víneis

Perera

2007

Cancer Epidemiology, Biomarkers &Amp; Prevention

162

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“…We, therefore, aimed to identify the radiationresponsive genes at the protein level using two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and matrix assisted laser desorption/ionization time of flight-mass spectrometry (MALDI-TOF-MS) in MCF-7 human breast cancer cells. Tools such as 2D-PAGE and MALDI-TOF-MS have been widely used for the study of cancer proteomics, as noted in several other studies (23)(24)(25).…”

Section: Introductionmentioning

confidence: 99%

Protein expression pattern in response to ionizing radiation in MCF-7 human breast cancer cells

Jung

Lee

et al. 2011

Oncology Letters

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Abstract. Breast cancer is one of the most common types of cancer in women and is highly treatable by radiotherapy. However, repeated exposure to radiation results in tumor cell resistance. Understanding the molecular mechanisms involved in the response of tumors to γ-irradiation is important for improving radiotherapy. For this reason, we aimed to identify radiation-responsive genes at the protein level. In the present study, we observed differentially expressed proteins using 2D-PAGE and MALDI-TOF-MS for the global analysis of protein expression patterns in response to ionizing radiation (IR). When the expression patterns of proteins were compared to a control gel, numerous spots were found that differed greatly. Among them, 11 spots were found to be significantly different. One set of proteins (GH2, RGS17, BAK1, CCNH, TSG6, RAD51B, IGFBP1 and CASP14) was upregulated and another set of proteins (C1QRF, PLSCR2 and p34 SE1-1 ) was downregulated after exposure to γ-rays. These proteins are known to be related to cell cycle control, apoptosis, DNA repair, cell proliferation and other signaling pathways.

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“…Other cancer tissues were analyzed using proteome methods, such as of lung [91,92], liver [93], colon [77,94], bladder [95][96][97][98], heart [99] or prostate [100]. Srisomsap et al [101] detected upregulation of cathepsin B in neoplastic thyroid tissues by proteomic analysis.…”

Section: Examples Of Successful Application Of Proteomicsmentioning

confidence: 99%

Two‐dimensional gel electrophoresis as tool for proteomics studies in combination with protein identification by mass spectrometry

2006

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The proteome analysis by 2-DE is one of the most potent methods of analyzing the complete proteome of cells, cell lines, organs and tissues in proteomics studies. It allows a fast overview of changes in cell processes by analysis of the entire protein extracts in any biological and medical research projects. New instrumentation and advanced technologies provide proteomics studies in a wide variety of biological and biomedical questions. Proteomics work is being applied to study antibiotics-resistant strains and human tissues of various brain, lung, and heart diseases. It cumulated in the identification of antigens for the design of new vaccines. These advances in proteomics have been possible through the development of advanced high-resolution 2-DE systems allowing resolution of up to 10 000 protein spots of entire cell lysates in combination with protein identification by new highly sensitive mass spectrometric techniques. The present technological achievements are suited for a high throughput screening of different cell situations. Proteomics may be used to investigate the health effects of radiation and electromagnetic field to clarify possible dangerous alterations in human beings.

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A database of protein expression in lung cancer

Cited by 80 publications

References 16 publications

Molecular Epidemiology and Biomarkers in Etiologic Cancer Research: The New in Light of the Old

Molecular Epidemiology and Biomarkers in Etiologic Cancer Research: The New in Light of the Old

Protein expression pattern in response to ionizing radiation in MCF-7 human breast cancer cells

Two‐dimensional gel electrophoresis as tool for proteomics studies in combination with protein identification by mass spectrometry

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