A decade of vibrational micro-spectroscopy of human cells and tissue (1994–2004)

Diem, Max; Romeo, Melissa; Boydston‐White, Susie; Miljković, Miloš D.; Matthäus, Christian

doi:10.1039/b408952a

Cited by 265 publications

(198 citation statements)

References 21 publications

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“…These spectral images are based solely on the similarity of the spectra in a hyperspectral data set. A detailed description of HCA has been published (Diem et al, 2004;Lasch et al, 2004;Wood et al, 2004). For HCA, the pair-wise similarity coeffcients of all spectra in a data set are collected as a matrix of correlation coeffcients C, which contains N 2 entries, where N is the total number of spectra in a data set.…”

Section: Principal Component Analysis-mentioning

confidence: 99%

“…Therefore, the possibility of using label-free imaging methods is of interest to the scientific community. Among the label-free methods, newly developed techniques of vibrational microspectroscopic imaging (Diem et al, 2004) have gained acceptance in many fields such as nanoscience and semiconductor technology; however, their presence and acceptance in cell biology has been limited. The two most common techniques of vibrational micro-spectroscopy are infrared (IR) and Raman (RA) microspectroscopy (IR-MSP and RA-MSP).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Chapter 10 Infrared and Raman Microscopy in Cell Biology

Matthäus

Bird

Miljković

et al. 2008

Methods in Cell Biology

168

119

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This chapter presents novel microscopic methods to monitor cell biological processes of live or fixed cells without the use of any dye, stains, or other contrast agent. These methods are based on spectral techniques that detect inherent spectroscopic properties of biochemical constituents of cells, or parts thereof. Two different modalities have been developed for this task. One of them is infrared micro-spectroscopy, in which an average snapshot of a cell's biochemical composition is collected at a spatial resolution of typically 25 mm. This technique, which is extremely sensitive and can collect such a snapshot in fractions of a second, is particularly suited for studying gross biochemical changes. The other technique, Raman microscopy (also known as Raman microspectroscopy), is ideally suited to study variations of cellular composition on the scale of subcellular organelles, since its spatial resolution is as good as that of fluorescence microscopy. Both techniques exhibit the fingerprint sensitivity of vibrational spectroscopy toward biochemical composition, and can be used to follow a variety of cellular processes.

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Section: Principal Component Analysis-mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Chapter 10 Infrared and Raman Microscopy in Cell Biology

Matthäus

Bird

Miljković

et al. 2008

Methods in Cell Biology

168

119

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show abstract

“…During the last 15 years, methods have been developed that can be viewed as molecular pathology methods that depend on sampling the entire genome, proteome and metabolome of cells and tissues, rather than probing for the presence of selected markers [11,12]. These methods rely on the detection of the inherent spectral signatures of the biochemical components in a pixel of tissue.…”

Section: Introductionmentioning

confidence: 99%

Molecular pathology via IR and Raman spectral imaging

Diem¹,

Mazur²,

Lenau³

et al. 2013

Journal of Biophotonics

177

115

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Journal of BIOPHOTONICSDuring the last 15 years, vibrational spectroscopic methods have been developed that can be viewed as molecular pathology methods that depend on sampling the entire genome, proteome and metabolome of cells and tissues, rather than probing for the presence of selected markers. First, this review introduces the background and fundamentals of the spectroscopies underlying the new methodologies, namely infrared and Raman spectroscopy. Then, results are presented in the context of spectral histopathology of tissues for detection of metastases in lymph nodes, squamous cell carcinoma, adenocarcinomas, brain tumors and brain metastases. Results from spectral cytopathology of cells are discussed for screening of oral and cervical mucosa, and circulating tumor cells. It is concluded that infrared and Raman spectroscopy can complement histopathology and reveal information that is available in classical methods only by costly and time-consuming steps such as immunohistochemistry, polymerase chain reaction or gene arrays. Due to the inherent sensitivity toward changes in the bio-molecular composition of different cell and tissue types, vibrational spectroscopy can even provide information that is in some cases superior to that of any one of the conventional techniques.Schematic of spectral histopathology (SHP) process: diagnostic algorithm is developed by spectral data of well documented specimens and subsequently applied to unknown dataset.

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“…Due to vibrational selection rules biological molecules may contain individual bonds or moieties that will be either infrared or Raman active, and consequently measurement of both spectra assesses the total content of organic chemical species within a biological specimen [19]. Both modalities have in the last decade seen an acceleration of their use in and application to biological research as a result of the development of microscope systems and solid state detection systems that now allow the rapid measurement of the chemical content in cellular and tissue species [20] within laboratory benchtop devices. The spatial resolution of both Fourier Transform Infrared microspectroscopy (FTIRM) and Confocal Raman Microscopy (CRM) is diffraction limited to ~3µm to 10µm in FTIRM and ~0.5µm to 1.5µm in CRM depending on the measurement wavelengths and detector characteristics in a given system [21].…”

Section: Introductionmentioning

confidence: 99%

Spectroscopic and chemometric approaches to radiobiological analyses

Meade

Byrne²,

Lyng

2010

Mutation Research/Reviews in Mutation Research

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Vibrational spectroscopy is an attractive modality for the analysis of biological samples, providing a complete non-invasive acquisition of the biochemical fingerprint of the sample. It has been demonstrated that this data provides the means to assay multiple functional responses of a biological system at a spatial resolution as low as a micron within the sample. As the interaction of ionizing radiation with biological systems involves chemical reactions between the products of radiation induced damage and various structural and functional units within the cell, the vibrational spectroscopic modalities have received attention as potential measurement platforms for the in-situ examination of the chemistry of biological species in radiobiology. This presents challenges in relation to sample preparation and the construction of suitable analytical methodologies. In this work protocols for sample preparation and approaches to multivariate analysis of vibrational spectra in radiobiological analysis are detailed and the utility of the methodology in analyzing the evolution of biochemical responses to radiobiological damage are highlighted.

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A decade of vibrational micro-spectroscopy of human cells and tissue (1994–2004)

Cited by 265 publications

References 21 publications

Chapter 10 Infrared and Raman Microscopy in Cell Biology

Chapter 10 Infrared and Raman Microscopy in Cell Biology

Molecular pathology via IR and Raman spectral imaging

Spectroscopic and chemometric approaches to radiobiological analyses

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