A decision support system to detect morphologic changes of chromatin arrangement in normal-appearing cells

Sacile, Roberto; Montaldo, Elisa; Ruggiero, Carmelina; Nieburgs, Herbert E.; G, Nicolò

doi:10.1109/tnb.2003.813939

Cited by 10 publications

(5 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…IF was analysed using the Automated QUantitative Analysis (AQUA) system (HistoRx, New Haven, CT) as previously described [ 69 ]. For each immunofluorescence image, AQUAnalysis software evaluated the quantity (in AQUA units=Au) of target protein expression (through Cy-5-tyramide) within the cytoplasm (identified by cytokeratin) and nuclei (DAPI).…”

Section: Methodsmentioning

confidence: 99%

The role of HDAC2 in chromatin remodelling and response to chemotherapy in ovarian cancer

et al. 2015

View full text Add to dashboard Cite

Chromatin undergoes structural changes in response to extracellular and environmental signals. We observed changes in nuclear morphology in cancer tissue biopsied after chemotherapy and hypothesised that these DNA damage-induced changes are mediated by histone deacetylases (HDACs). Nuclear morphological changes in cell lines (PE01 and PE04 models) and a xenograft model (OV1002) were measured in response to platinum chemotherapy by image analysis of nuclear texture. HDAC2 expression increased in PEO1 cells treated with cisplatin at 24h, which was accompanied by increased expression of heterochromatin protein 1 (HP1). HDAC2 and HP1 expression were also increased after carboplatin treatment in the OV1002 carboplatin-sensitive xenograft model but not in the insensitive HOX424 model. Expression of DNA damage response pathways (pBRCA1, γH2AX, pATM, pATR) showed time-dependent changes after cisplatin treatment. HDAC2 knockdown by siRNA reduced HP1 expression, induced DNA double strand breaks (DSB) measured by γH2AX, and interfered with the activation of DNA damage response induced by cisplatin. Furthermore, HDAC2 depletion affected γH2AX foci formation, cell cycle distribution, and apoptosis triggered by cisplatin, and was additive to the inhibitory effect of cisplatin in cell lines. By inhibiting expression of HDAC2, reversible alterations in chromatin patterns during cisplatin treatment were observed. These results demonstrate quantifiable alterations in nuclear morphology after chemotherapy, and implicate HDAC2 in higher order chromatin changes and cellular DNA damage responses in ovarian cancer cells in vitro and in vivo.

show abstract

Section: Methodsmentioning

confidence: 99%

The role of HDAC2 in chromatin remodelling and response to chemotherapy in ovarian cancer

et al. 2015

View full text Add to dashboard Cite

show abstract

“…MACs refer to biochemical changes, such as chromatin rearrangement, in visually and histologically normal cells in the vicinity of a tumor that can be detected with ultra sensitive techniques like high resolution cytometry. This idea was first proposed over 40 years ago [37][38][39], and more recent studies of tissues such as lung [40][41][42], cervix [43][44][45][46], and breast [47][48][49] have supported the idea.…”

Section: Malignancy Associated Changesmentioning

confidence: 99%

Detecting Temporal and Spatial Effects of Epithelial Cancers with Raman Spectroscopy

et al. 2008

View full text Add to dashboard Cite

Epithelial cancers, including those of the skin and cervix, are the most common type of cancers in humans. Many recent studies have attempted to use Raman spectroscopy to diagnose these cancers. In this paper, Raman spectral markers related to the temporal and spatial effects of cervical and skin cancers are examined through four separate but related studies. Results from a clinical cervix study show that previous disease has a significant effect on the Raman signatures of the cervix, which allow for near 100% classification for discriminating previous disease versus a true normal. A Raman microspectroscopy study showed that Raman can detect changes due to adjacent regions of dysplasia or HPV that cannot be detected histologically, while a clinical skin study showed that Raman spectra may be detecting malignancy associated changes in tissues surrounding nonmelanoma skin cancers. Finally, results of an organotypic raft culture study provided support for both the skin and the in vitro cervix results. These studies add to the growing body of evidence that optical spectroscopy, in this case Raman spectral markers, can be used to detect subtle temporal and spatial effects in tissue near cancerous sites that go otherwise undetected by conventional histology.

show abstract

“…Several studies have demonstrated the phenomenon. [3][4][5] MACs are subtle sub-visual, i.e. visually imperceptible, changes in the appearance of otherwise normal-looking cells from an abnormal Pap slide.…”

Section: Introductionmentioning

confidence: 99%

Novel chromatin texture features for the classification of pap smears

et al. 2013

View full text Add to dashboard Cite

This paper presents a set of novel structural texture features for quantifying nuclear chromatin patterns in cells on a conventional Pap smear. The features are derived from an initial segmentation of the chromatin into bloblike texture primitives. The results of a comprehensive feature selection experiment, including the set of proposed structural texture features and a range of different cytology features drawn from the literature, show that two of the four top ranking features are structural texture features. They also show that a combination of structural and conventional features yields a classification performance of 0.954±0.019 (AUC±SE) for the discrimination of normal (NILM) and abnormal (LSIL and HSIL) slides. The results of a second classification experiment, using only normal-appearing cells from both normal and abnormal slides, demonstrates that a single structural texture feature measuring chromatin margination yields a classification performance of 0.815±0.019. Overall the results demonstrate the efficacy of the proposed structural approach and that it is possible to detect malignancy associated changes (MACs) in Papanicoloau stain.

show abstract

A decision support system to detect morphologic changes of chromatin arrangement in normal-appearing cells

Cited by 10 publications

References 13 publications

The role of HDAC2 in chromatin remodelling and response to chemotherapy in ovarian cancer

The role of HDAC2 in chromatin remodelling and response to chemotherapy in ovarian cancer

Detecting Temporal and Spatial Effects of Epithelial Cancers with Raman Spectroscopy

Novel chromatin texture features for the classification of pap smears

Contact Info

Product

Resources

About