A deep insight into the male and female sialotranscriptome of adult Culex tarsalis mosquitoes

Ribeiro, José M. C.; Martín-Martín, Inés; Moreira, Fernando R.; Bernard, Kristen A.; Calvo, Eric

doi:10.1016/j.ibmb.2018.03.001

Cited by 23 publications

(28 citation statements)

References 67 publications

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“…D7 proteins are among the most abundant components in the salivary glands of several blood-feeding arthropods and are distantly related to the arthropod odorant-binding protein superfamily [7][8][9][10] . The D7s belong to a multi-gene family that evolved through gene duplication events, resulting in long forms and truncated versions of a duplicated long form, known as short forms 8 .…”

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confidence: 99%

ADP binding by the Culex quinquefasciatus mosquito D7 salivary protein enhances blood feeding on mammals

et al. 2020

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During blood-feeding, mosquito saliva is injected into the skin to facilitate blood meal acquisition. D7 proteins are among the most abundant components of the mosquito saliva. Here we report the ligand binding specificity and physiological relevance of two D7 long proteins from Culex quinquefasciatus mosquito, the vector of filaria parasites or West Nile viruses. CxD7L2 binds biogenic amines and eicosanoids. CxD7L1 exhibits high affinity for ADP and ATP, a binding capacity not reported in any D7. We solve the crystal structure of CxD7L1 in complex with ADP to 1.97 Å resolution. The binding pocket lies between the two protein domains, whereas all known D7s bind ligands either within the Nor the C-terminal domains. We demonstrate that these proteins inhibit hemostasis in ex vivo and in vivo experiments. Our results suggest that the ADP-binding function acquired by CxD7L1 evolved to enhance blood-feeding in mammals, where ADP plays a key role in platelet aggregation.

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confidence: 99%

ADP binding by the Culex quinquefasciatus mosquito D7 salivary protein enhances blood feeding on mammals

et al. 2020

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“…This phenomenon is not unique to RPW and has also been confirmed in many other insects, such as T. castaneum [3], T. molitor [8], Nicrophorus vespilloide [37], and Helicoverpa armigera [34]. OS are produced by salivary glands [35]. In the present study, we correspondingly detected the specific overexpression of all RfARSBs in salivary glands, the mRNA abundance of which was much higher than that in other tissues/organs, including the head, epidermis, fat body, gut, and hemolymph.…”

Section: Discussionmentioning

confidence: 64%

“…In recent years, this phenomenon has also been found to be increasingly common in other insects [33,34]. Previously, a sialotranscriptome of adult female, Culex tarsalis, revealed that oral secretions (OS) were generally produced by salivary glands [35].…”

Section: Introductionmentioning

confidence: 96%

Identification of Novel ARSB Genes Necessary for p-Benzoquinone Biosynthesis in the Larval Oral Secretion Participating in External Immune Defense in the Red Palm Weevil

Zhang

et al. 2020

IJMS

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External secretions, composed of a variety of chemical components, are among the most important traits that endow insects with the ability to defend themselves against predators, parasites, or other adversities, especially pathogens. Thus, these exudates play a crucial role in external immunity. Red palm weevil larvae are prolific in this regard, producing large quantities of p-benzoquinone, which is present in their oral secretion. Benzoquinone with antimicrobial activity has been proven to be an active ingredient and key factor for external immunity in a previous study. To obtain a better understanding of the genetic and molecular basis of external immune secretions, we identify genes necessary for p-benzoquinone synthesis. Three novel ARSB genes, namely, RfARSB-0311, RfARSB-11581, and RfARSB-14322, are screened, isolated, and molecularly characterized on the basis of transcriptome data. To determine whether these genes are highly and specifically expressed in the secretory gland, we perform tissue/organ-specific expression profile analysis. The functions of these genes are further determined by examining the antimicrobial activity of the secretions and quantification of p-benzoquinone after RNAi. All the results reveal that the ARSB gene family can regulate the secretory volume of p-benzoquinone by participating in the biosynthesis of quinones, thus altering the host’s external immune inhibitory efficiency.

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“…Additional SNPs in the reference sequence compared to multiple sequenced KNWR individuals were removed by extracting the consensus sequence from a single male KNWR library mapped to the reference using bcftools. Annotations were performed using Maker (v.2.31.10) with the Culex tarsalis transcriptome (Ribeiro et al 2018) and Cx. quinquefasciatus protein sequences (CpipJ2.4).…”

Section: Pacbio Genome Assemblymentioning

confidence: 99%

Whole genome assembly ofCulex tarsalis

Main

Marcantonio

Johnston

et al. 2020

Preprint

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The mosquito, Culex tarsalis, is a key vector species in the western United States due to its role in transmission of zoonotic arboviruses that affect human health. Extensive research has been conducted on Cx. tarsalis ecology, feeding behavior, vector competence, autogeny, diapause, genetics, and insecticide resistance. However, the lack of a published reference genome has limited genomic analyses for this species, although a previous population genetic analysis of microsatellite allele frequencies across the western U.S. identified three geographic clusters. Salivary gland-specific gene expression has also revealed genes involved in blood feeding. To facilitate genomic studies in this important vector species, we have assembled and annotated a reference genome (CtarK1) based on PacBio HiFi reads from a single male and 10X linked-reads. Genome completeness was 71.5% based on the Benchmarking Universal Single-Copy Orthologs (BUSCO) tool and the N50 was 59 kb. The largest contig was 5.7Mb, including 678kb of unknown bases. CtarK1 is 37% larger than Cx. quinquefasciatus (800Mb vs. 579Mb); a difference that is largely transposable elements. Using the Cx. tarsalis transcriptome and protein sequences from Culex quinquefasciatus, 15,682 genes were annotated in the CtarK1 genome. Based on full mitochondrial genome alignments we present a Bayesian phylogeny and estimate the divergence time from Culex quinquefasciatus at 13 MYA (+/-4).

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A deep insight into the male and female sialotranscriptome of adult Culex tarsalis mosquitoes

Cited by 23 publications

References 67 publications

ADP binding by the Culex quinquefasciatus mosquito D7 salivary protein enhances blood feeding on mammals

ADP binding by the Culex quinquefasciatus mosquito D7 salivary protein enhances blood feeding on mammals

Identification of Novel ARSB Genes Necessary for p-Benzoquinone Biosynthesis in the Larval Oral Secretion Participating in External Immune Defense in the Red Palm Weevil

Whole genome assembly ofCulex tarsalis

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