A Deep Learning-Based System (Microscan) for the Identification of Pollen Development Stages and Its Application to Obtaining Doubled Haploid Lines in Eggplant

García‐Fortea, Edgar; Garcı́a-Pérez, Ana L.; Gimeno-Páez, Esther; Sánchez-Gimeno, Alfredo; Vilanova, Santiago; Prohens, Jaime; Pastor-Calle, David

doi:10.3390/biology9090272

Cited by 6 publications

(6 citation statements)

References 64 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The second relevant parameter that determines a successful induction of microspore embryogenesis is the stage of the microspores used for in vitro culture, either through anther culture or through microspore culture. It is widely acknowledged that the best stage to induce embryogenesis revolves around the first pollen mitosis [13,33,36]. This means that, in general, mature, vacuolated microspores and young, just divided pollen grains are the stages where embryogenesis can be induced more efficiently.…”

Section: Anther Culture In Eggplantmentioning

confidence: 99%

See 1 more Smart Citation

Doubled Haploids in Eggplant

Mir

Calabuig-Serna

Seguí-Simarro

2021

Biology

View full text Add to dashboard Cite

Eggplant is a solanaceous crop cultivated worldwide for its edible fruit. Eggplant breeding programs are mainly aimed to the generation of F1 hybrids by crossing two highly homozygous, pure lines, which are traditionally obtained upon several self crossing generations, which is an expensive and time consuming process. Alternatively, fully homozygous, doubled haploid (DH) individuals can be induced from haploid cells of the germ line in a single generation. Several attempts have been made to develop protocols to produce eggplant DHs principally using anther culture and isolated microspore culture. Eggplant could be considered a moderately recalcitrant species in terms of ability for DH production. Anther culture stands nowadays as the most valuable technology to obtain eggplant DHs. However, the theoretical possibility of having plants regenerated from somatic tissues of the anther walls cannot be ruled out. For this reason, the use of isolated microspores is recommended when possible. This approach still has room for improvement, but it is largely genotype-dependent. In this review, we compile the most relevant advances made in DH production in eggplant, their application to breeding programs, and the future perspectives for the development of other, less genotype-dependent, DH technologies.

show abstract

Section: Anther Culture In Eggplantmentioning

confidence: 99%

“…m 2019 [32] Role of the cell wall in the embryogenic response of different species, including eggplant. m 2020 [33] Procedure for the identification of the microspore/pollen responsive stages.…”

Section: Introductionmentioning

confidence: 99%

Doubled Haploids in Eggplant

Mir

Calabuig-Serna

Seguí-Simarro

2021

Biology

View full text Add to dashboard Cite

show abstract

“…Pollen has been the primary target of these types of approaches, likely because it is relatively easy to isolate compared to the female gametophyte. Previous studies have used deep learning to identify pollen from several different species (Dunker et al 2020 ) and differentiate pollen developmental stages (García-Fortea et al 2020 ).…”

Section: Opportunities For High-throughput Phenotyping In Plant Reproductionmentioning

confidence: 99%

Deep learning-based high-throughput phenotyping can drive future discoveries in plant reproductive biology

Warman

Fowler

2021

Plant Reprod

View full text Add to dashboard Cite

Key message Advances in deep learning are providing a powerful set of image analysis tools that are readily accessible for high-throughput phenotyping applications in plant reproductive biology. High-throughput phenotyping systems are becoming critical for answering biological questions on a large scale. These systems have historically relied on traditional computer vision techniques. However, neural networks and specifically deep learning are rapidly becoming more powerful and easier to implement. Here, we examine how deep learning can drive phenotyping systems and be used to answer fundamental questions in reproductive biology. We describe previous applications of deep learning in the plant sciences, provide general recommendations for applying these methods to the study of plant reproduction, and present a case study in maize ear phenotyping. Finally, we highlight several examples where deep learning has enabled research that was previously out of reach and discuss the future outlook of these methods.

show abstract

“…The generative cell then divides to form two sperm cells (pollen mitosis II, PMII), resulting in a tricellular pollen grain . The developmental stage of the donor pollen is a key factor for the successful induction of ME, and the window of developmental stages with embryogenic competence is very narrow (García-Fortea et al, 2020;. In most species, including B. napus, haploid embryos are thought to develop from the mid to late-unicellular microspore (just before pollen mitosis I) or from the vegetative cell of the bicellular pollen (just after pollen mitosis I) .…”

Section: From Microspore To Haploid Embryomentioning

confidence: 99%

“…Abiotic or chemical stress treatments can induce immature male gametophytes of many plants to change developmental pathways in vitro from pollen to haploid embryo development, a process commonly referred to as microspore embryogenesis (ME) . The developmental stage of the donor pollen is a key factor for induction of ME and the window of developmental stages with embryogenic competence is very narrow (García-Fortea et al, 2020;. In B. napus, the mid-unicellular microspore stage (just before pollen mitosis I) to the early-bicellular pollen stage (just after pollen mitosis I) are the most responsive developmental stages for embryo induction Kyo & Harada, 1986;Pechan & Keller, 1988;Telmer et al, 1992).…”

Section: Introductionmentioning

confidence: 99%

From microspore to haploid embryo : Dissecting the key molecular and cellular factors driving microspore embryogenesis

Siemons

View full text Add to dashboard Cite

The main aim of this thesis is to dissect the key molecular and cellular factors involved in Brassica napus in vitro microspore embryogenesis. Microspore embryogenesis is a form of in vitro totipotency where the male gametophyte is reprogrammed to form haploid embryos, usually after exposure to an abiotic stress treatment. Induced embryogenic structures can follow different developmental pathways that can be similar or very different to zygotic embryogenesis. In this Chapter, a comparison is made between zygotic embryogenesis and in vitro microspore embryogenesis. The similarities and differences between these two types of embryogenesis are discussed, focusing on their development and transcriptional regulation.The role of the hormone auxin and the role of histone acetylation are also discussed in detail.Totipotency is defined as the ability of a single cell to form an entire organism . The single-celled zygote is the only totipotent cell that can form an entire organism during normal sexual reproduction. Remarkably, many other plant cells are also totipotent and develop into embryos in the absence of fertilization, either via asexual reproduction or in vitro in response to an inducer treatment . Somatic embryogenesis (SE) is a form of plant cell totipotency where embryos are formed from vegetative cells, generating plants with the same genetic composition and ploidy level as the donor plant (i.e., clones). SE occurs naturally in the ovule tissues of some apomictic plants and along the leaf margins of other plants (Garcês et al., 2007;Ozias-Akins & Conner, 2020), but can also be induced in vitro in many different cells in a wide range of model and crop plants . In addition to SE, both male and female gametophytic cells can also form embryos without fertilization in a process called gametophytic embryogenesis (Reynolds, 1997; Soriano et al., 2013b). In some apomictic species, diploid embryos develop spontaneously from an egg cell formed without meiotic recombination or chromosome reduction (parthenogenesis) .Isolated haploid gametophytic cells can also be induced in vitro to form haploid embryos, but in this case the embryos are derived from meiotically produced cells and are genetically distinct from the mother plant. Haploid embryogenesis from the female gametophyte/egg cell, as in apomicts, is called gynogenesis or parthenogenesis , while haploid embryogenesis from the male gametophyte/pollen grain is termed androgenesis or microspore embryogenesis (ME) (Dong et al., 2021;Hale et al., 2022;. In both cases, the resulting haploid embryo can be converted into a Embryo proper development begins with a transverse division of the distal-most suspensor cell, which continues to divide like the typical ordered zygotic embryo .Suspensors can also develop as small protrusions or with multiple cell files , which are observed in the low response genotype DH12075 used in this study. However, it is not known if these embryos develop in a similar way to suspensor embryos in Topas DH4079 cultures, i.e., first the development of...

show abstract

A Deep Learning-Based System (Microscan) for the Identification of Pollen Development Stages and Its Application to Obtaining Doubled Haploid Lines in Eggplant

Cited by 6 publications

References 64 publications

Doubled Haploids in Eggplant

Doubled Haploids in Eggplant

Deep learning-based high-throughput phenotyping can drive future discoveries in plant reproductive biology

From microspore to haploid embryo : Dissecting the key molecular and cellular factors driving microspore embryogenesis

Contact Info

Product

Resources

About