A dehydration‐induced NAC protein, RD26, is involved in a novel ABA‐dependent stress‐signaling pathway

Fujita, Miki; Fujita, Yasunari; Maruyama, Kyonoshin; Seki, Motoaki; Hiratsu, Keiichiro; Ohme‐Takagi, Masaru; Tran, Lam‐Son Phan; Yamaguchi-Shinozaki, Kazuko; Shinozaki, Kazuo

doi:10.1111/j.1365-313x.2004.02171.x

Cited by 842 publications

(641 citation statements)

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“…NAM) [11], stress responses (eg. ATAF1, RD26 and OsNAC6) [18,47,66] and leaf senescence (eg. NAP) [29].…”

Section: Resultsmentioning

confidence: 99%

“…NAC proteins have been implicated to participate in a wide range of plant developmental processes, including shoot apical meristem development [10,23-26], floral morphogenesis [27], lateral root development [15,28], leaf senescence [29,30], stress inducible flowering induction [31,32], embryo development [11], cell cycle control [33-35], hormone signaling [15,18,33,36], grain nutrient remobilization [30,37] and shoot branching determination [38]. Particularly, numerous NAC domain proteins have also been implicated in plant abiotic stresses and defense responses such as drought, salinity, cold shock, mechanical wounding and viral infection [18,20,28,36,39-47].…”

Section: Introductionmentioning

confidence: 99%

“…Particularly, numerous NAC domain proteins have also been implicated in plant abiotic stresses and defense responses such as drought, salinity, cold shock, mechanical wounding and viral infection [18,20,28,36,39-47]. Recently, accumulating evidences indicated that a considerable portion of NAC domain proteins also play crucial roles in the processes of xylogenesis, fiber development, and wood formation in vascular plants [17,19,21,48-55].…”

Section: Introductionmentioning

confidence: 99%

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Comprehensive Analysis of NAC Domain Transcription Factor Gene Family in Populus trichocarpa

et al. 2010

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BackgroundNAC (NAM, ATAF1/2 and CUC2) domain proteins are plant-specific transcriptional factors known to play diverse roles in various plant developmental processes. NAC transcription factors comprise of a large gene family represented by more than 100 members in Arabidopsis, rice and soybean etc. Recently, a preliminary phylogenetic analysis was reported for NAC gene family from 11 plant species. However, no comprehensive study incorporating phylogeny, chromosomal location, gene structure, conserved motifs, and expression profiling analysis has been presented thus far for the model tree species Populus.ResultsIn the present study, a comprehensive analysis of NAC gene family in Populus was performed. A total of 163 full-length NAC genes were identified in Populus, and they were phylogeneticly clustered into 18 distinct subfamilies. The gene structure and motif compositions were considerably conserved among the subfamilies. The distributions of 120 Populus NAC genes were non-random across the 19 linkage groups (LGs), and 87 genes (73%) were preferentially retained duplicates that located in both duplicated regions. The majority of NACs showed specific temporal and spatial expression patterns based on EST frequency and microarray data analyses. However, the expression patterns of a majority of duplicate genes were partially redundant, suggesting the occurrence of subfunctionalization during subsequent evolutionary process. Furthermore, quantitative real-time RT-PCR (RT-qPCR) was performed to confirm the tissue-specific expression patterns of 25 NAC genes.ConclusionBased on the genomic organizations, we can conclude that segmental duplications contribute significantly to the expansion of Populus NAC gene family. The comprehensive expression profiles analysis provides first insights into the functional divergence among members in NAC gene family. In addition, the high divergence rate of expression patterns after segmental duplications indicates that NAC genes in Populus are likewise to have been retained by substantial subfunctionalization. Taken together, our results presented here would be helpful in laying the foundation for functional characterization of NAC gene family and further gaining an understanding of the structure-function relationship between these family members.

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“…NAM) [11], stress responses (eg. ATAF1, RD26 and OsNAC6) [18,47,66] and leaf senescence (eg. NAP) [29].…”

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Comprehensive Analysis of NAC Domain Transcription Factor Gene Family in Populus trichocarpa

et al. 2010

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“…In Brassica napus, nine members of the NAC (BnNAC) were identified for their differential expression after feeding with flea beetle and treatment of cold temperature (Hegedus et al 2003). In Arabidopsis, Tran et al (2004) and Fujita et al (2004) reported that three NAC genes ANAC019, ANAC055 and ANAC072 were induced by drought, salinity, and/or low temperature, and the transgenic Arabidopsis plants overexpressing these genes showed improved stress tolerance compared to the wild type. Furthermore, Proteins of these genes can bind to the promoter sequences with CATGTG motif .…”

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confidence: 99%

Characterization of transcription factor gene SNAC2 conferring cold and salt tolerance in rice

et al. 2008

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Plants respond to adverse environment by initiating a series of signaling processes including activation of transcription factors that can regulate expression of arrays of genes for stress response and adaptation. NAC (NAM, ATAF, and CUC) is a plant specific transcription factor family with diverse roles in development and stress regulation. In this report, a stress-responsive NAC gene (SNAC2) isolated from upland rice IRA109 (Oryza sativa L. ssp japonica) was characterized for its role in stress tolerance. SNAC2 was proven to have transactivation and DNA-binding activities in yeast and the SNAC2-GFP fusion protein was localized in the rice nuclei. Northern blot and SNAC2 promoter activity analyses suggest that SNAC2 gene was induced by drought, salinity, cold, wounding, and abscisic acid (ABA) treatment. The SNAC2 gene was over-expressed in japonica rice Zhonghua 11 to test the effect on improving stress tolerance. More than 50% of the transgenic plants remained vigorous when all WT plants died after severe cold stress (4-8°C for 5 days). The transgenic plants had higher cell membrane stability than wild type during the cold stress. The transgenic rice had significantly higher germination and growth rate than WT under high salinity conditions. Over-expression of SNAC2 can also improve the tolerance to PEG treatment. In addition, the SNAC2-overexpressing plants showed significantly increased sensitivity to ABA. DNA chip profiling analysis of transgenic plants revealed many up-regulated genes related to stress response and adaptation such as peroxidase, ornithine aminotransferase, heavy metal-associated protein, sodium/hydrogen exchanger, heat shock protein, GDSL-like lipase, and phenylalanine ammonia lyase. Interestingly, none of the up-regulated genes in the SNAC2-overexpressing plants matched the genes up-regulated in the transgenic plants over-expressing other stress responsive NAC genes reported previously. These data suggest SNAC2 is a novel stress responsive NAC transcription factor that possesses potential utility in improving stress tolerance of rice.

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“…For example, transcript accumulation was observed for RD26 in response to cold stress [44], but this was not found with ATAF1 (data not shown). Recently, OsNAC6, a rice ortholog of ATAF1, was reported to be a positive contributor to disease resistance and salinity tolerance [29,30].…”

Section: Functional Overlap Between Ataf1 and Other Stressregulatory mentioning

confidence: 92%

Dual function of Arabidopsis ATAF1 in abiotic and biotic stress responses

et al. 2009

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NAC family genes encode plant-specific transcription factors involved in diverse biological processes. In this study, the Arabidopsis NAC gene ATAF1 was found to be induced by drought, high-salinity, abscisic acid (ABA), methyl jasmonate, mechanical wounding, and Botrytis cinerea infection. Significant induction of ATAF1 was found in an ABA-deficient mutant aba2 subjected to drought or high salinity, revealing an ABA-independent mechanism of expression. Arabidopsis ATAF1-overexpression lines displayed many altered phenotypes, including dwarfism and short primary roots. Furthermore, in vivo experiments indicate that ATAF1 is a bona fide regulator modulating plant responses to many abiotic stresses and necrotrophic-pathogen infection. Overexpression of ATAF1 in Arabidopsis increased plant sensitivity to ABA, salt, and oxidative stresses. Especially, ATAF1 overexpression plants, but not mutant lines, showed remarkably enhanced plant tolerance to drought. Additionally, ATAF1 overexpression enhanced plant susceptibility to the necrotrophic pathogen B. cinerea, but did not alter disease symptoms caused by avirulent or virulent strains of P. syringae pv tomato DC3000. Transgenic plants overexpressing ATAF1 were hypersensitive to oxidative stress, suggesting that reactive oxygen intermediates may be related to ATAF1-mediated signaling in response to both pathogen and abiotic stresses.

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A dehydration‐induced NAC protein, RD26, is involved in a novel ABA‐dependent stress‐signaling pathway

Cited by 842 publications

References 79 publications

Comprehensive Analysis of NAC Domain Transcription Factor Gene Family in Populus trichocarpa

Comprehensive Analysis of NAC Domain Transcription Factor Gene Family in Populus trichocarpa

Characterization of transcription factor gene SNAC2 conferring cold and salt tolerance in rice

Dual function of Arabidopsis ATAF1 in abiotic and biotic stress responses

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