A demonstration of athermal effects of continuous microwave irradiation on the growth and antibiotic sensitivity of<i>Pseudomonas aeruginosa</i>PAO1

Nakouti, Ismini; Hobbs, Glyn; Teethaisong, Yothin; Phipps, D. A.

doi:10.1002/btpr.2392

Cited by 11 publications

(15 citation statements)

References 33 publications

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“…This observation coincides with the previous reports shown that exposure to electromagnetic fields (EMF) makes some bacteria resistant to antibiotics [8] and the effects of EMF exposure depend on the morphology of exposed cells [20]. Moreover, continuous 24 hours exposure to mobile 1.8 GHz waves showed the presence of persisters Pseudomonas aeruginosa cells with enhanced antibiotic resistance [11]. In contrast, no significant effects of high frequency electromagnetic fields were revealed on antibiotic sensitivity of exposed Staphylococcus [7].…”

Section: Discussionsupporting

confidence: 90%

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Evaluation of Wi-Fi Radiation Effects on Antibiotic Susceptibility, Metabolic Activity and Biofilm Formation by Escherichia Coli O157H7, Staphylococcus Aureus and Staphylococcus Epidermis

et al. 2019

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Background:The radiation emitted from electromagnetic fields (EMF) can cause biological effects on prokaryotic and eukaryotic cells, including non-thermal effects. Objective: The present study evaluated the non-thermal effects of wireless fidelity (Wi-Fi) operating at 2.4 GHz part of non-ionizing EMF on different pathogenic bacterial strains (Escherichia coli 0157H7, Staphylococcus aureus, and Staphylococcus epidermis). Antibiotic resistance, motility, metabolic activity and biofilm formation were examined. Material and Methods: In this case-control, a Wi-Fi router was used as a source of microwaves and also bacterial cells were exposed to Wi-Fi radiation continuously for 24 and 48 hours. The antibiotic susceptibility was carried out using a disc diffusion method on Müller Hinton agar plates. Motility of Escherichia coli 0157H7 was conducted on motility agar plates. Cell metabolic activity and biofilm formation were performed using 3-(4, 5-Dimethylthiazol-2yl)-2, 5-diphenyltetrazolium bromide (MTT) assay and crystal violet quantification, respectively. Results:The exposure to Wi-Fi radiation altered motility and antibiotic susceptibility of Escherichia coli 0157H7. However, there was no effect Wi-Fi radiation on antibiotic susceptibility of Staphylococcus aureus and Staphylococcus epidermis. On the other hand, the exposed cells, as compared to the unexposed control, showed an increased metabolic activity and biofilm formation ability in Escherichia coli 0157H7, Staphylococcus aureus and Staphylococcus epidermis. Conclusion:These results proposed that Wi-Fi exposure acted on bacteria in stressful manner by increasing antibiotic resistance and motility of Escherichia coli 0157H7, as well as enhancing biofilm formation by Escherichia coli 0157H7, Staphylococcus aureus and Staphylococcus epidermis. The findings may have implications for the management of serious diseases caused by these infectious bacteria.

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Section: Discussionsupporting

confidence: 90%

“…Several studies investigated the non-thermal effects of high frequency electromagnetic fields (GSM and Wi-Fi) on different strains of bacteria [5-11]. Exposure of Staphylococcus aureus , Staphylococcus epidermis , and Pseudomonas aeruginosa to GSM revealed no effects on their bacterial growth rate or antibiotic susceptibility [7].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Wi-Fi Radiation Effects on Antibiotic Susceptibility, Metabolic Activity and Biofilm Formation by Escherichia Coli O157H7, Staphylococcus Aureus and Staphylococcus Epidermis

et al. 2019

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“…Suspension turbidity of E. coli (p<0.05) When bacteria are exposed to 2.4 GHz Wi-Fi for a long time, it has been shown to affect the growth of bacteria [20]. In our study, the growth of E. coli (25922) after 6 h exposure was negatively affected from the 5 GHz Wi-Fi that corrects long term exposure results of previous studies [22,23]. SH-SY5Y cells have been used for in vitro studies of neurodegenerative disorders.…”

Section: Resultssupporting

confidence: 86%

5 GHZ Wi Fi Effects on Escherichia Coli, Caenorhabditis Elegans and Human Neuroblastoma Cells

Dinç

Ilyas

Kaygusuz

et al. 2021

Mugla Journal of Science and Technology

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The use of 5 GHz Wi-Fi has spread and potential effects on microorganisms and human health are still under investigation. To investigate the possible effects for that, experiments were performed using three different microorganisms, Escherichia coli, SH-SY5Y human neuroblastoma cells and Caernohabditis elegans. The maximum allowed temperature for the Wi-Fi Access Point (AP) was considered in the measurement setup in order not to harm the internal circuitry of the router which can result impact the performance of the router. Suspension turbidity, optical density measurements and simultaneous counting of E. coli were performed. It was observed that 5 GHz Wi-Fi exposure slow down the E. coli growth rate at the same temperature conditions with sham exposed groups especially after the first 6 h. 5 GHz Wi-Fi induced decrease in cell viability remarkably for the cells seeded at densities 20,40, 100 (×10 3) and viability values were varied between 20 and 30%. C. elegans which is a nematode, plays key role in nutrient cycling and soil fertilization was affected by the radiation and egg-laying rates changed by 27.49%.

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“…The exposure of S. aureus to mobile phone for one hour did not affect their ability to form biofilm 3 . Longer time of GSM and Wi-Fi exposure affected the growth, antibiotic susceptibility and biofilm formation of P. aeruginosa, E. coli, S. aureus, S. epidermis and L. monocytogenes 6,7,9 . The prolonged EMF exposure resulted in major effects in K. pneumonia before they reach an adaptation stage 5 .…”

Section: Discussionmentioning

confidence: 99%

Global gene expression analysis of Escherichia coli K-12 DH5α after exposure to 2.4 GHz wireless fidelity radiation

Said-Salman

Jebaii

Yusef

et al. 2019

Sci Rep

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This study investigated the non-thermal effects of Wi-Fi radiofrequency radiation of 2.4 GHz on global gene expression in Escherichia coli K-12 DH5α. High-throughput RNA-sequencing of 2.4 GHz exposed and non-exposed bacteria revealed that 101 genes were differentially expressed (DEGs) at P ≤ 0.05. The up-regulated genes were 52 while the down-regulated ones were 49. QRT-PCR analysis of pgaD, fliC, cheY, malP, malZ, motB, alsC, alsK, appB and appX confirmed the RNA-seq results. About 7% of DEGs are involved in cellular component organization, 6% in response to stress stimulus, 6% in biological regulation, 6% in localization, 5% in locomotion and 3% in cell adhesion. Database for annotation, visualization and integrated discovery (DAVID) functional clustering revealed that DEGs with high enrichment score included genes for localization of cell, locomotion, chemotaxis, response to external stimulus and cell adhesion. Kyoto encyclopedia of genes and genomes (KEGG) pathways analysis showed that the pathways for flagellar assembly, chemotaxis and two-component system were affected. Go enrichment analysis indicated that the up-regulated DEGs are involved in metabolic pathways, transposition, response to stimuli, motility, chemotaxis and cell adhesion. The down-regulated DEGs are associated with metabolic pathways and localization of ions and organic molecules. Therefore, the exposure of E. coli DH5α to Wi-Fi radiofrequency radiation for 5 hours influenced several bacterial cellular and metabolic processes.

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A demonstration of athermal effects of continuous microwave irradiation on the growth and antibiotic sensitivity ofPseudomonas aeruginosaPAO1

Cited by 11 publications

References 33 publications

Evaluation of Wi-Fi Radiation Effects on Antibiotic Susceptibility, Metabolic Activity and Biofilm Formation by Escherichia Coli O157H7, Staphylococcus Aureus and Staphylococcus Epidermis

Evaluation of Wi-Fi Radiation Effects on Antibiotic Susceptibility, Metabolic Activity and Biofilm Formation by Escherichia Coli O157H7, Staphylococcus Aureus and Staphylococcus Epidermis

5 GHZ Wi Fi Effects on Escherichia Coli, Caenorhabditis Elegans and Human Neuroblastoma Cells

Global gene expression analysis of Escherichia coli K-12 DH5α after exposure to 2.4 GHz wireless fidelity radiation

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