A dense and strong bonding collagen film for carbon/carbon composites

Cao, Sheng; Li, Hejun; Li, Kezhi; Lu, Jinhua; Zhang, Leilei

doi:10.1016/j.apsusc.2015.04.081

Cited by 11 publications

(12 citation statements)

References 34 publications

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“…First, C/C composites were modified by 30% H 2 O 2 for 4 h at 100°C, then they were washed and vacuum dried . Second, modified C/C specimens were immersed in the C‐collagen solution, and then extracted the residual air for half an hour.…”

Section: Methodsmentioning

confidence: 99%

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In vitro mineralization of MC3T3‐E1 osteoblast‐like cells on collagen/nano‐hydroxyapatite scaffolds coated carbon/carbon composites

Cao

et al. 2015

J Biomedical Materials Res

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Collagen/nano-hydroxyapatite (collagen/nHA) scaffolds were successfully prepared on carbon/carbon composites as bioactive films using the layer-by-layer coating method. Surface characterizations of collagen/nHA scaffolds were detected by scanning electron microscope (SEM), X-ray diffraction (XRD), and Fourier transform infrared (FTIR) spectroscopy. Compressive strengths of the scaffolds were evaluated by a universal test machine. In vitro biological performances were determined using scaffolds seeded with MC3T3-E1 osteoblasts-like cells and cultured in mineralization medium for up to 21 days. In addition, cellular morphologies and several related gene expressions of MC3T3-E1 cells in the scaffolds were also evaluated. Chemical and morphological analysis showed that the scaffolds had uniform pore sizes and unified phase composition. Mechanical testing indicated that the collagen/nHA scaffolds had the highest compressive strength in 50% of strain condition when the proportion of collagen and nano-hydroxyapatite was 1:3. Cellular morphology observations and cytology tests indicated that MC3T3-E1 cells were adhered on these scaffolds and proliferated. SEM photographs and gene expressions showed that mineralized MC3T3-E1 cells and newly formed extra cellular matrix (ECM) filled up the pores of the scaffolds after the 3-week mineralization inducement. Nano-sized apatite particles were secreted from MC3T3-E1 cells and combined with the reconstructed ECM. Collectively, collagen/nHA scaffolds provided C/C composites with a biomimetic surface for cell adhesion, proliferation and mineralized extra cellular matrices formation.

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Section: Methodsmentioning

confidence: 99%

“…In our previous work, a dense and strong bonding collagen coating had been successfully applied on C/C composites . This collagen film could serve also as a transition film between collagen/nHA scaffolds and C/C composites.…”

Section: Introductionmentioning

confidence: 99%

In vitro mineralization of MC3T3‐E1 osteoblast‐like cells on collagen/nano‐hydroxyapatite scaffolds coated carbon/carbon composites

Cao

et al. 2015

J Biomedical Materials Res

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“…In our previous research, numerous hydroxyl, carboxyl, and carbonyl functional groups were formed on the surface of an H-C/C sample [12]. The improvement in surface wettability enhanced the adhesion numbers of MC3T3-E1 cells on the H-C/C surface.…”

Section: Discussionmentioning

confidence: 99%

“…After a 4-hour treatment, the modified C/C samples were taken out, then washed with deionized water three times, and dried in a vacuum environment. The oxidized C/C samples were considered as H-C/C samples [12].…”

Section: Methodsmentioning

confidence: 99%

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Unique cytological behavior of MC3T3-E1 osteoblasts on H2O2-modified C/C composites in vitro

Cao

et al. 2017

Sci. China Mater.

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MC3T3-E1 osteoblasts were cultured on H2O2-modified and unmodified carbon/carbon (H-C/C and C/C) composites for one week in order to evaluate differences in cell adhesion, spreading, and proliferation. The results indicated a certain degree of enhancement in the cell adhesion capability of osteoblasts cultured on H-C/C samples. Cellular morphologies after cell adhesion were observed via scanning electron microscopy (SEM), which showed that the cells adhered more closely and spread more widely on the H-C/C sample surface. However, no cell appeared in several multiple and continuous types of minor pores on both the C/C and H-C/C surfaces. In addition, two unique situations were observed on the H-C/C samples: an outline change of the osteoblasts was observed when the cells spread across some minor pores, and the cells entered and adhered well in some larger pores.

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