A derivative of the menaquinone precursor 1,4-dihydroxy-2-naphthoate is involved in the reductive transformation of carbon tetrachloride by aerobically grown Shewanella oneidensis MR-1

Ward, Melissa J.; Fu, Qing; Rhoads, Kurt R.; Yeung, Chok Hang; Spormann, Alfred M.; Criddle, Craig S.

doi:10.1007/s00253-003-1407-3

Cited by 30 publications

(23 citation statements)

References 15 publications

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“…The fact that menaquinone rescued reduction of the Fe-beads by a menF mutant of MR-1 but did not stimulate Fe-bead reduction by wild-type cells demonstrated that the mediator used for iron reduction at a distance is not a product of the menaquinone biosynthetic pathway. This rules out the previously described quinone-like molecule derived from the menaquinone pathway (48,67) as an electron shuttle for iron reduction at a distance.…”

Section: Discussionmentioning

confidence: 99%

Shewanella oneidensis MR-1 Uses Overlapping Pathways for Iron Reduction at a Distance and by Direct Contact under Conditions Relevant for Biofilms

Lies¹,

Hernández

Kappler³

et al. 2005

Appl Environ Microbiol

304

199

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We developed a new method to measure iron reduction at a distance based on depositing Fe(III) (hydr)oxide within nanoporous glass beads. In this "Fe-bead" system, Shewanella oneidensis reduces at least 86.5% of the iron in the absence of direct contact. Biofilm formation accompanies Fe-bead reduction and is observable both macro-and microscopically. Fe-bead reduction is catalyzed by live cells adapted to anaerobic conditions, and maximal reduction rates require sustained protein synthesis. The amount of reactive ferric iron in the Fe-bead system is available in excess such that the rate of Fe-bead reduction is directly proportional to cell density; i.e., it is diffusion limited. Addition of either lysates prepared from anaerobic cells or exogenous electron shuttles stimulates Fe-bead reduction by S. oneidensis, but iron chelators or additional Fe(II) do not. Neither dissolved Fe(III) nor electron shuttling activity was detected in culture supernatants, implying that the mediator is retained within the biofilm matrix. Strains with mutations in omcB or mtrB show about 50% of the wild-type levels of reduction, while a cymA mutant shows less than 20% of the wild-type levels of reduction and a menF mutant shows insignificant reduction. The Fe-bead reduction defect of the menF mutant can be restored by addition of menaquinone, but menaquinone itself cannot stimulate Fe-bead reduction. Because the menF gene encodes the first committed step of menaquinone biosynthesis, no intermediates of the menaquinone biosynthetic pathway are used as diffusible mediators by this organism to promote iron reduction at a distance. CymA and menaquinone are required for both direct and indirect mineral reduction, whereas MtrB and OmcB contribute to but are not absolutely required for iron reduction at a distance.

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Section: Discussionmentioning

confidence: 99%

Shewanella oneidensis MR-1 Uses Overlapping Pathways for Iron Reduction at a Distance and by Direct Contact under Conditions Relevant for Biofilms

Lies¹,

Hernández

Kappler³

et al. 2005

Appl Environ Microbiol

304

199

View full text Add to dashboard Cite

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“…In addition, a number of radionucleotide oxides can be reduced (Myers and Nealson, 1988;Nealson and Scott, 2003;Icopini et al, 2009). Thus, bacteria such as Shewanella significantly impact biogeochemical cycling processes and are of particular interest with regard to bioremediation processes (Heidelberg et al, 2002;Nealson et al, 2002;Lovley et al, 2004;Ward et al, 2004;Hau and Gralnick, 2007). It has been hypothesized that direct interaction of Shewanella cells with, or close proximity to, an appropriate surface facilitates the deposition of electrons (Das and Caccavo, 2000;Gorby et al, 2006;McLean et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Phage-induced lysis enhances biofilm formation in Shewanella oneidensis MR-1

et al. 2010

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Shewanella oneidensis MR-1 is capable of forming highly structured surface-attached communities. By DNase I treatment, we demonstrated that extracellular DNA (eDNA) serves as a structural component in all stages of biofilm formation under static and hydrodynamic conditions. We determined whether eDNA is released through cell lysis mediated by the three prophages LambdaSo, MuSo1 and MuSo2 that are harbored in the genome of S. oneidensis MR-1. Mutant analyses and infection studies revealed that all three prophages may individually lead to cell lysis. However, only LambdaSo and MuSo2 form infectious phage particles. Phage release and cell lysis already occur during early stages of static incubation. A mutant devoid of the prophages was significantly less prone to lysis in pure culture. In addition, the phage-less mutant was severely impaired in biofilm formation through all stages of development, and three-dimensional growth occurred independently of eDNA as a structural component. Thus, we suggest that in S. oneidensis MR-1 prophage-mediated lysis results in the release of crucial biofilm-promoting factors, in particular eDNA.

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“…Previously, we reported a genetic analysis of surface adhesion and biofilm formation, as well as a physiological and molecular characterization of detachment in Shewanella oneidensis MR-1, which is a facultative Fe(III) and Mn(IV) mineral-reducing soil bacterium and plays a critical role in mineral dissolution, heavy-metal (im)mobilization, and pollution degradation (31,33,47,48,52). In a genetic screen for biofilm defective mutants, we identified type IV pili as critical in mediating the initial adhesion of cells to the substratum during surface colonization (47).…”

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confidence: 99%

Control of Formation and Cellular Detachment from Shewanella oneidensis MR-1 Biofilms by Cyclic di-GMP

Thormann¹,

Duttler²,

Saville³

et al. 2006

J Bacteriol

219

203

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Stability and resilience against environmental perturbations are critical properties of medical and environmental biofilms and pose important targets for their control. Biofilm stability is determined by two mutually exclusive processes: attachment of cells to and detachment from the biofilm matrix. Using Shewanella oneidensis MR-1, an environmentally versatile, Fe(III) and Mn(IV) mineral-reducing microorganism, we identified mxdABCD as a new set of genes essential for formation of a three-dimensional biofilm. Molecular analysis revealed that mxdA encodes a cyclic bis(3,5)guanylic acid (cyclic di-GMP)-forming enzyme with an unusual GGDEF motif, i.e., NVDEF, which is essential for its function. mxdB encodes a putative membrane-associated glycosyl transferase. Both genes are essential for matrix attachment. The attachment-deficient phenotype of a ⌬mxdA mutant was rescued by ectopic expression of VCA0956, encoding another diguanylate cyclase. Interestingly, a rapid cellular detachment from the biofilm occurred upon induction of yhjH, a gene encoding an enzyme that has been shown to have phosphodiesterase activity. In this way, it was possible to bypass the previously identified sudden depletion of molecular oxygen as an environmental trigger to induce biofilm dissolution. We propose a model for c-di-GMP as a key intracellular regulator for controlling biofilm stability by shifting the state of a biofilm cell between attachment and detachment in a concentration-dependent manner.

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A derivative of the menaquinone precursor 1,4-dihydroxy-2-naphthoate is involved in the reductive transformation of carbon tetrachloride by aerobically grown Shewanella oneidensis MR-1

Cited by 30 publications

References 15 publications

Shewanella oneidensis MR-1 Uses Overlapping Pathways for Iron Reduction at a Distance and by Direct Contact under Conditions Relevant for Biofilms

Shewanella oneidensis MR-1 Uses Overlapping Pathways for Iron Reduction at a Distance and by Direct Contact under Conditions Relevant for Biofilms

Phage-induced lysis enhances biofilm formation in Shewanella oneidensis MR-1

Control of Formation and Cellular Detachment from Shewanella oneidensis MR-1 Biofilms by Cyclic di-GMP

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