A detailed restriction endonuclease cleavage map of rat liver mitochondrial DNA

Feldmann, Horst; Grosskopf, Rüdiger

doi:10.1007/bf00413303

Curr Genet

1979

DOI: 10.1007/bf00413303

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A detailed restriction endonuclease cleavage map of rat liver mitochondrial DNA

Horst Feldmann

Rüdiger Grosskopf²

Abstract: A restriction endonuclease cleavage map of rat liver mitochondrial DNA is presented for the following enzymes: Xba I, Bgl II, Hae II, Bam HI, Hpa I, Hha I, Bcl I, Hind II, Hind III, Eco RI, Hpa II, Hae III, and Sau 3A. It was derived from complete and partial digestions with these enzymes, double digestions, and redigestions of defined fragments obtained with one enzyme with other restriction enzymes. By the use of these and further enzymes (Taq YI, Alu I) the mitochondrial DNA (ca. 15.6 Kb) can be dissected i… Show more

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1981

1982

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Cited by 9 publications

References 25 publications

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Polymorphisms of mitochondrial DNAs in Norway rats (Rattus norvegicus): Cleavage site variations and length polymorphism of restriction fragments

et al. 1981

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Extensive polymorphism was found in mitochondrial DNAs (mtDNAs) of Norway rats (Rattus norvegicus). The restriction endonuclease cleavage patterns of mtDNAs of laboratory rats, wild rats, tumor cells, and culture cells were compared. The polymorphism is defined by two criteria; one is cleavage site variation and the other is length polymorphism of restriction fragments. The cleavage site variation may be caused by point mutation, and the length polymorphism by sequence deletions or insertions. At least five types, types A-E, were identified by cleavage site variations, and two groups, a and b, were identified by length polymorphism of one HpaII fragment, Hpa5. All types except type C belonged to either group-a or group-b, whereas both groups were found in type C. Differentiation of polymorphic Norway rat mtDNA types and the experimental use of the polymorphism are discussed.

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Polymorphisms of mitochondrial DNAs in Norway rats (Rattus norvegicus): Cleavage site variations and length polymorphism of restriction fragments

et al. 1981

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Analysis of a DNA segment from rat liver mitochondria containing the genes for the cytochrome oxidase subunits I, II and III, ATPase subunit 6, and several tRNA genes

Grosskopf

Feldmann

1981

Current Genetics

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The sequence of a 6.24 kb DNA segment of the mitochondrial genome from rat liver has been determined. It comprises several genes coding for mitochondrial protein subunits and five tRNA genes in the following order: cytochrome oxidase subunit I - tRNA (UCN) (Ser) -tRNA(Asp) - cytochrome oxidase subunit II - tRNALys -ATPase subunit - cytochrome oxidase subunit III -tRNA(Gly) - potential open reading frame - tRNA(Arg) -two potential open reading frames. The tRNA genes were detected by a computer search programme. The assignments for the protein coding sequences were made through comparison with known sequences, mainly from the yeast mitochondrial proteins (e.g. Bonitz et al. 1980). Our data are discussed with regard to the features of gene arrangement, codon usage, and tRNA structure in mammalian mitochondria (Anderson et al. 1981).

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Nucleotide sequence of the cytochrome B gene and adjacent regions from rat liver mitochondrial DNA

Görtz

Feldmann

1982

Curr Genet

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The nucleotide sequence of a 1.76 Kbp segment from rat liver mitochondrial DNA has been determined. It contains the gene for the cytochrome b apoprotein and the tRNA genes for threonine, glutamic acid, and proline. The arrangement of these genes in rat liver mitochondria is the same as in human (Anderson et al. 1981) or mouse (Bibb et al. 1981) mitochondria. The comparison of the cytochrome b sequences so far determined, reveals that several regions in these proteins are highly conserved.

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A detailed restriction endonuclease cleavage map of rat liver mitochondrial DNA

Cited by 9 publications

References 25 publications

Polymorphisms of mitochondrial DNAs in Norway rats (Rattus norvegicus): Cleavage site variations and length polymorphism of restriction fragments

Polymorphisms of mitochondrial DNAs in Norway rats (Rattus norvegicus): Cleavage site variations and length polymorphism of restriction fragments

Analysis of a DNA segment from rat liver mitochondria containing the genes for the cytochrome oxidase subunits I, II and III, ATPase subunit 6, and several tRNA genes

Nucleotide sequence of the cytochrome B gene and adjacent regions from rat liver mitochondrial DNA

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