A detergent-polyacrylamide gel system for electrophoretic resolution of membrane and wall proteins

Cell-Envelope Proteins of Bordetella Pertussis

“…With other bacteria this technique has proved to be very useful for separating and comparing envelope polypeptides according to molecular weight (e.g. Schaitman, 1970a and b ; Grula and Savoy, 1971). We have therefore used SDS-polysacrylamide-gel electrophoresis to compare the protein profiles of B. pertussis phase-I (X-mode) cell envelopes with those of phase IV and C-mode.…”

Properties of a Thermosensitive Asporogenous Filamentous Mutant of Bacillus megaterium

“…The electrophoresis buffer consisted of sodium phosphate buffer (0.1 M and pH 7.0) containing SDS (0.1% wt/vol). Gels were pre-run at 4 mA/gel for 1 h to remove ammonium persulfate and other low-molecular-weight materials.…”

“…(i) It was assumed that the proteins were equally extractable from the membranes of either strain grown at permissive or restrictive conditions. The extraction procedure was rigorous and commonly used in these kinds of comparisons (4,9,18). (ii) The complete disag- Effect of penicillin on growth of rods and filaments of ts mutant TH14.…”

“…However, some of the membrane protein profiles of Escherichia coli by Siccardi et al (20) do resemble those reported here. The protein profile obtained is dependent on the method of protoplast ghost membrane preparation, and the method of Salton and Freer (17) was not used for the published profiles referred to above (4,9,10,18,20). It is unlikely that the quantity of fraction Z is an artifact due to random heat-induced rupture of peptide bonds during extraction since its Effect of temperature shift-up on sporulation of ts mutant TH14.…”