2000
DOI: 10.1523/jneurosci.20-03-01260.2000
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A Developmental Change in NMDA Receptor-Associated Proteins at Hippocampal Synapses

Abstract: The membrane-associated guanylate kinases [Chapsyn-110/postsynaptic density-93 (PSD-93), synapse-associated protein-90 (SAP-90)/PSD-95, and SAP-102] are believed to cluster and anchor NMDA receptors at the synapse and to play a role in signal transduction. We have investigated the developmental changes in expression of these proteins in rat hippocampus using biochemical analyses and quantitative immunogold electron microscopy. At postnatal day 2 (P2), SAP-102 was highly expressed, whereas PSD-93 and PSD-95 wer… Show more

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Cited by 472 publications
(554 citation statements)
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“…In this study, we examined the developmental changes in GluRs and several associated proteins in synapses. Previous studies showed that 1) NMDA receptors are high from P2-adult, while AMPA receptors increase with age , and 2) the NMDA receptor-associated MAGUK, SAP102, decreases with age while other MAGUKs, PSD-95 and PSD-93, increase with age (Sans et al, 2000). Here we show that those early postnatal synapses that display ultrastructural signs of maturity already have acquired substantial levels of many GluRassociated proteins.…”
Section: Introductionsupporting
confidence: 62%
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“…In this study, we examined the developmental changes in GluRs and several associated proteins in synapses. Previous studies showed that 1) NMDA receptors are high from P2-adult, while AMPA receptors increase with age , and 2) the NMDA receptor-associated MAGUK, SAP102, decreases with age while other MAGUKs, PSD-95 and PSD-93, increase with age (Sans et al, 2000). Here we show that those early postnatal synapses that display ultrastructural signs of maturity already have acquired substantial levels of many GluRassociated proteins.…”
Section: Introductionsupporting
confidence: 62%
“…Three antibodies to mGluR5 were obtained from Chemicon International (Temecula, CA, USA); another from Chemicon was made to the last 13 amino acids and recognizes both mGluR5 and mGluR1alpha. Antibodies to AMPA receptors, NR1, NR2A/B, PSD-95, PSD-93, SAP97 and SAP102 were characterized in general and for immunogold labeling in hippocampus by our lab (Petralia and Wenthold, 1992;Petralia et al, 1994aPetralia et al, ,b, 1999Sans et al, 2000Sans et al, , 2001. Some new labeling studies were carried out for NR1 (amino acids 909-938; affinity-purified; recognizes the intracellular domain), SAP97 (amino acids 1-105; previous published studies used both this antiserum and an identical affinity-purified antibody), and SAP102 (amino acids 1-119; antiserum; previous published studies used an identical affinity-purified antibody).…”
Section: Antibodies and Their Characterizationmentioning
confidence: 99%
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“…The double-band detected for PSD-93, in accordance with earlier reports, likely represent different splice variants of this protein. 62,63 Correlation analysis, using age, pH and PMI as independent measures, identified significant associations between PMI and NF-L expression in DLPFC (r = 0.50, P = 0.02) and PSD-93 and PMI in ACC (r = 0.45, P = 0.04). In addition, we found PSD-95 expression in ACC negatively associated with pH (r = 0.44, 0.048) (i.e., lower pH = increased PSD-95 concentration).…”
Section: Resultsmentioning
confidence: 99%
“…SAP102 is expressed prior to PSD95 and accumulates first at hippocampal synapses in vivo [35,36]. Each molecule has differential effects during synaptogenesis and synapse maturation.…”
Section: Discussionmentioning
confidence: 99%