A developmentally regulated wound epithelial antigen of the newt limb regenerate is also present in a variety of secretory/transport cell types

Goldhamer, David J.; Tomlinson, Bruce L.; Tassava, Roy A.

doi:10.1016/0012-1606(89)90188-7

Cited by 17 publications

(11 citation statements)

References 30 publications

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“…The cells of the basal layer are the closest epithelial cells to the distal mesenchyme of the blastema, and studies suggest that these cells have robust secretory activity (Singer and Salpeter, 1961;Goldhamer et al, 1989). Here, we show that FN message in the AEC is expressed only in the cells of the basal layer, and previous work showed FN protein strongly immunolocalized beneath the basal layer (Nace and Tassava, 1995); thus, FN protein appears to be secreted into the basal lamina, mesenchyme, or both, from these cells.…”

Section: Differences Between Aec Compartmentsmentioning

confidence: 99%

“…The outermost cornified layer and the basal-most low columnar layer bound the inner layer(s) of cuboidal cells (Singer and Salpeter, 1961). Ultrastructural and immunocytochemical studies suggest a robust secretory activity, especially in the basal layer (Singer and Salpeter, 1961;Tassava et al, 1986;Goldhamer et al, 1989;Estrada et al, 1993). The AEC secretes factors needed by the underlying blastema mesenchyme, providing critical stimulatory signals for regeneration (reviewed in Stocum, 1995).…”

Section: Introductionmentioning

confidence: 95%

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Apical epithelial cap morphology and fibronectin gene expression in regenerating axolotl limbs

Christensen

Tassava

2000

Dev. Dyn.

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Section: Differences Between Aec Compartmentsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 95%

Apical epithelial cap morphology and fibronectin gene expression in regenerating axolotl limbs

Christensen

Tassava

2000

Dev. Dyn.

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“…The wound epidermis expresses two antigens designated WE3 and WE6 that are thought to be actin‐binding proteins and to regulate secretion and/or ionic composition (Castilla & Tassava, 1992; Estrada, Park, Castilla, & Tassava, 1993; Goldhamer, Tomlinson, & Tassava, 1989; Tassava & Acton, 1989; Tassava, Castilla, Arsanto, & Thouveny, 1993; Tassava, Johnson‐Wint, & Gross, 1986). These antigens are not expressed in uninjured epidermis, indicating that they are specific to regeneration.…”

Section: Formation Of the Accumulation Blastemamentioning

confidence: 99%

Mechanisms of urodele limb regeneration

Stocum

2017

Regeneration

109

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This review explores the historical and current state of our knowledge about urodele limb regeneration. Topics discussed are (1) blastema formation by the proteolytic histolysis of limb tissues to release resident stem cells and mononucleate cells that undergo dedifferentiation, cell cycle entry and accumulation under the apical epidermal cap. (2) The origin, phenotypic memory, and positional memory of blastema cells. (3) The role played by macrophages in the early events of regeneration. (4) The role of neural and AEC factors and interaction between blastema cells in mitosis and distalization. (5) Models of pattern formation based on the results of axial reversal experiments, experiments on the regeneration of half and double half limbs, and experiments using retinoic acid to alter positional identity of blastema cells. (6) Possible mechanisms of distalization during normal and intercalary regeneration. (7) Is pattern formation is a self‐organizing property of the blastema or dictated by chemical signals from adjacent tissues? (8) What is the future for regenerating a human limb?

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“…It has long been thought that the WE is secretory in nature , 32,33 and it is apparent that ECM proteins represent one set of secreted molecules. It is impor tant to determine why these ECM proteins are made by the mesenchyme and the WE 16 and to what degree WE secretion of ECM proteins functions in the process of regeneration .…”

Section: Synthesis Of Extracellular Matrix Components By Wound Epithementioning

confidence: 99%

Extracellular matrix protein turnover during salamander limb regeneration

Tassava

Nace

Wei

1996

Wound Repair Regeneration

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After amputation of a salamander limb, the extracellular matrix undergoes remodeling. The extracellular matrix that maintains the differentiated state of limb tissues is broken down and replaced by an extracellular matrix essential for dedifferentiation and blastema formation. We used monoclonal antibodies in immunohistochemistry methods and riboprobes in in situ hybridization to evaluate the upregulation of tenascin, type XII collagen, fibronectin, and the MT5 antigen. The Stump 1 antigen, an extracellular matrix protein that is abundant in the normal limb, is downregulated during regeneration and reappears late in regeneration as differentiation occurs. In the embryo, the Stump 1 antigen is also absent from the early limb bud and first appears during differentiation stages. Tenascin and fibronectin are also upregulated in the limb bud of the embryo, and these two extracellular matrix proteins appear to function during limb regeneration in adults and limb development in embryos. However, type XII collagen and the MT5 antigen are not found in the limb bud, indicating that type XII collagen and the MT5 antigen have roles in the regenerating limb but not in the embryo limb bud.

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A developmentally regulated wound epithelial antigen of the newt limb regenerate is also present in a variety of secretory/transport cell types

Cited by 17 publications

References 30 publications

Apical epithelial cap morphology and fibronectin gene expression in regenerating axolotl limbs

Apical epithelial cap morphology and fibronectin gene expression in regenerating axolotl limbs

Mechanisms of urodele limb regeneration

Extracellular matrix protein turnover during salamander limb regeneration

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