A di- O -dihydrogeranylgeranyl glycerol from Thermococcus S 557, a novel ether lipid, and likely intermediate in the biosynthesis of diethers in Archæa

Gonthier, Isabelle; Rager, Marie‐Noëlle; Metzger, Pierre; Guézennec, Jean; Largeau, Claude

doi:10.1016/s0040-4039(01)00305-7

Cited by 27 publications

(17 citation statements)

References 22 publications

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“…III). These results confirmed the presence of unsaturated archaeol, consistent with the previously studied structure of 2,3-di-O-dihydrogeranylgeranyl glycerol diether (31).…”

Section: Nmr Analysis Of Total Polar Lipidssupporting

confidence: 92%

Positive and negative tandem mass spectrometric fingerprints of lipids from the halophilic Archaea Haloarcula marismortui

Souza

Müller-Santos

Iacomini

et al. 2009

Journal of Lipid Research

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Lipids from the extremely halophilic Archaea, Haloarcula marismortui, contain abundant phytanyl diether phospholipids, namely archaetidic acid (AA), archaetidylglycerol (AG), archaetidylglycerosulfate (AGS), with mainly archaetidylglycerophosphate methyl ester (AGP-Me). These were accompanied by a triglycosyl archaeol (TGA), lacking characteristic sulfate groups. Tandem-mass spectrometry was employed to provide fingerprints for identifying these known lipids, as well as small amounts of unsaturated phospholipids. These contained 3 and 6 double bonds in their archaeol moiety, suggested by negative tandem-MS of intact phospholipids, as indicated by differences between their pseudo-molecular ion and specific fragment ions designated as p 2 . The core ether lipids were confirmed by electrospray ionization mass spectrometry (ESI-MS) as 2,3-di-O-phytanylsn-glycerol (C20, C20), which gave rise to a precursor-ion at

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“…III). These results confirmed the presence of unsaturated archaeol, consistent with the previously studied structure of 2,3-di-O-dihydrogeranylgeranyl glycerol diether (31).…”

Section: Nmr Analysis Of Total Polar Lipidssupporting

confidence: 92%

Positive and negative tandem mass spectrometric fingerprints of lipids from the halophilic Archaea Haloarcula marismortui

Souza

Müller-Santos

Iacomini

et al. 2009

Journal of Lipid Research

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“…Unsaturated AR derivatives have so far not been described for the Thermoplasmatales, however, for euryarchaea of the orders Halobacteriales, Thermococcales, Methanopyrales and Methanosarcinales (e.g. Dawson et al, 2012;Gonthier et al, 2001;Hafenbradl et al, 1993;Nichols et al, 2004), with a broad range of growth temperatures. This indicates that the abundance of these compounds in strains S5…”

Section: Strains S5mentioning

confidence: 99%

The novel extremely acidophilic, cell-wall-deficient archaeon Cuniculiplasma divulgatum gen. nov., sp. nov. represents a new family, Cuniculiplasmataceae fam. nov., of the order Thermoplasmatales

Golyshina

Lünsdorf

Kublanov

et al. 2016

International Journal of Systematic and Evolutionary Microbiology

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Two novel cell-wall-less, acidophilic, mesophilic, organotrophic and facultatively anaerobic archaeal strains were isolated from acidic streamers formed on the surfaces of copper-orecontaining sulfidic deposits in south-west Spain and North Wales, UK. Cells of the strains varied from 0.1 to 2 mm in size and were pleomorphic, with a tendency to form filamentous structures. The optimal pH and temperature for growth for both strains were 1.0-1.2 and 37-40 8C, with the optimal substrates for growth being beef extract (3 g l 21) for strain S5 T and beef extract with tryptone (3 and 1 g l 21, respectively) for strain PM4. The lipid composition was dominated by intact polar lipids consisting of a glycerol dibiphytanyl glycerol tetraether (GDGT) core attached to predominantly glycosidic polar headgroups. In addition, free GDGT and small relative amounts of intact and core diether lipids were present. Strains S5T and PM4 possessed mainly menaquinones with minor fractions of thermoplasmaquinones. The DNA G+C content was 37.3 mol% in strain S5 T and 37.16 mol% for strain PM4. A similarity matrix of 16S rRNA gene sequences (identical for both strains) showed their affiliation to the order Thermoplasmatales, with 73.9-86.3 % identity with sequences from members of the order with validly published names. The average nucleotide identity between genomes of the strains determined in silico was 98.75 %, suggesting, together with the 16S rRNA gene-based phylogenetic analysis, that the strains belong to the same species. A novel family, Cuniculiplasmataceae fam. nov., genus Cuniculiplasma gen. nov. and species Cuniculiplasma divulgatum sp. nov. are proposed based on the phylogenetic, chemotaxonomic analyses and physiological properties of the two isolates, S5 T and PM4 (5JCM 306415VKM B-2940). TheThe first genus and species of the Thermoplasmatales, Thermoplasma acidophilum, was described in 1970 (Darland et al., 1970), followed 25 years later by the genus Picrophilus Schleper et al., 1995). Since then, the taxa Ferroplasma (Golyshina et al., 2000), Thermogymnomonas acidicola (Itoh et al., 2007) and Acidiplasma (Golyshina et al., 2009) were described in the first decade of the 21st century. All members of the Thermoplasmatales are characterized as micro-organisms that are difficult to isolate, having extremely acidic pH optima for growth (among the lowest known), with their cells being typically pleomorphic (with the only exception being members of Picrophilus), as a consequence of the lack of an intact cell wall.Abbreviations: GDD, glycerol dibiphytanyl diether; GDGT, glycerol dibiphytanyl glycerol tetraether; IPL, intact polar lipid.The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of strains PM4 and S5 T are KT005320 and KT005321.

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“…(3) The crosslinking of the two acyl-chains of the lipids to yield macrocyclic archaeol or caldarchaeol derivatives by a covalent bond between the isoprenoid chains reduces the motion of the molecule creating a more closely-packed structure and increasing the membrane stability, creating an efficient barrier against water, proton and solute leakage (Dannenmuller et al 2000;Mathai et al 2001). (4) The increase in unsaturation along the isoprenoid chains of the lipids as a function of temperature (Nichols et al 2004) or salinity (Dawson et al 2012) has to date only been described in the psychrophilic methanogen Methanococcoides burtonii (Franzmann et al 1992;Nichols et al 2004), but unsaturated lipids have been characterized in several species of hyperthermophiles (Hafenbradl et al 1993;Gonthier et al 2001), which might indicate the occurrence of a similar adaptive strategy in hydrothermal vent organisms.…”

Section: Physiology and Adaptationmentioning

confidence: 99%

Microbial diversity and adaptation to high hydrostatic pressure in deep-sea hydrothermal vents prokaryotes

et al. 2015

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Prokaryotes inhabiting in the deep sea vent ecosystem will thus experience harsh conditions of temperature, pH, salinity or high hydrostatic pressure (HHP) stress. Among the fifty-two piezophilic and piezotolerant prokaryotes isolated so far from different deep-sea environments, only fifteen (four Bacteria and eleven Archaea) that are true hyper/thermophiles and piezophiles have been isolated from deep-sea hydrothermal vents; these belong mainly to the Thermococcales order. Different strategies are used by microorganisms to thrive in deep-sea hydrothermal vents in which "extreme" physico-chemical conditions prevail and where non-adapted organisms cannot live, or even survive. HHP is known to impact the structure of several cellular components and functions, such as membrane fluidity, protein activity and structure. Physically the impact of pressure resembles a lowering of temperature, since it reinforces the structure of certain molecules, such as membrane lipids, and an increase in temperature, since it will also destabilize other structures, such as proteins. However, universal molecular signatures of HHP adaptation are not yet known and are still to be deciphered.

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A di- O -dihydrogeranylgeranyl glycerol from Thermococcus S 557, a novel ether lipid, and likely intermediate in the biosynthesis of diethers in Archæa

Cited by 27 publications

References 22 publications

Positive and negative tandem mass spectrometric fingerprints of lipids from the halophilic Archaea Haloarcula marismortui

Positive and negative tandem mass spectrometric fingerprints of lipids from the halophilic Archaea Haloarcula marismortui

The novel extremely acidophilic, cell-wall-deficient archaeon Cuniculiplasma divulgatum gen. nov., sp. nov. represents a new family, Cuniculiplasmataceae fam. nov., of the order Thermoplasmatales

Microbial diversity and adaptation to high hydrostatic pressure in deep-sea hydrothermal vents prokaryotes

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