A diagnostic method to detect alcelaphine herpesvirus-1 of malignant catarrhal fever using the polymerase chain reaction

Hsu, David S.; Shih, Ling-Na; Castro, Anthony E.; Zee, Y. C.

doi:10.1007/bf01310755

Cited by 21 publications

(13 citation statements)

References 21 publications

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“…Using culture grown virus, PCR was 100 times more sensitive than tissue culture isolation in detecting FHV-1, with a detection limit of 390 fg of viral DNA after ethidium bromide staining. This sensitivity is comparable to that previously reported for PCR assays for bovine herpesvirus 4 and alcelaphine herpesvirus 1 [15,17]. However, it was less sensitive than PCR/Southern blotting assays for human herpesviruses simplex and type 6 [3,5].…”

Section: Discussionsupporting

confidence: 83%

Detection of active and latent feline herpesvirus 1 infections using the polymerase chain reaction

Reubel

Ramos

Hickman

et al. 1993

Archives of Virology

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A polymerase chain reaction (PCR) assay was developed to detect the thymidine kinase gene of feline herpesvirus 1 (FHV-1) and to study the active and latent carrier state in a group of naturally FHV-1 infected specific pathogen free (SPF) cats. The detection limit of PCR products on ethidium bromide stained gels was 390 fg or about 3 x 10(3) copies of the FHV-1 genome. The PCR was 25% more sensitive than conventional cell culture based virus isolation techniques in detecting FHV-1 in oral/ocular swabs and 100 times more sensitive in detecting virus in cell culture supernatants. Sites of FHV-1 latency in FHV-1 carriers as determined by PCR were mainly tissues of the head, especially the trigeminal ganglia, optic nerves, olfactory bulbs and corneas. Oral fauces, salivary glands, lacrimal glands, cerebellum and conjunctiva were less consistently positive. The cerebral cortex, thymus, trachea, lung, liver, spleen, kidney, and peripheral blood mononuclear cells were consistently negative for FHV-1 genome. The distribution of FHV-1 DNA in the tissues of the head was similar whether or not corticosteroid-induced virus shedding was occurring at the time the tissues were collected. Infectious virus was never recovered from tissue homogenates regardless of the PCR status of the tissues.

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Section: Discussionsupporting

confidence: 83%

Detection of active and latent feline herpesvirus 1 infections using the polymerase chain reaction

Reubel

Ramos

Hickman

et al. 1993

Archives of Virology

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“…The high degree of sensitivity afforded by the PCR has provided the technology for the detection of rare DNA sequences and has been used extensively for viral detection and diagnosis. A PCR for the detection of AHV-1 DNA sequence [41], has been reported for the diagnosis of AHV infections [13,14]. The sensitivity of this nested PCR was reported to be 0.01 TCIDs0 of infective AHV-1 virus [ 14].…”

Section: Discussionmentioning

confidence: 99%

“…The PCR has been applied to the detection of other herpesviruses and a PCR test has been described for the detection of AHV-1 [13,14]. A 2-stage nested amplification reaction, utilising different primer pairs from the same AHV-1 cloned sequence, subsequently was shown to amplify both AHV-1 and AHV-2 isolates [ 14].…”

Section: Introductionmentioning

confidence: 99%

PCR detection of the sheep-associated agent of malignant catarrhal fever

Baxter

Pow

Bridgen

et al. 1993

Archives of Virology

230

229

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From a genomic library previously constructed from a lymphoblastoid cell line (LCL) propagated from a bovine case of sheep-associated malignant catarrhal fever (SA-MCF), caused by ovine herpesvirus-2 (OHV-2), several OHV-2 clones were identified and characterised by hybridisation using probes from the unique region of the Alcelaphine herpesvirus-1 (AVH-1) genome. Nucleotide sequence from one clone was generated and the predicted amino acid sequence was found to contain regions of homology with the 140 and 160 kDa tegument proteins of Epstein-Barr virus and herpesvirus saimiri respectively. Oligonucleotide primers were constructed and a polymerase chain reaction (PCR) test was developed for the detection of OHV-2 viral DNA. Amplified product was identified by restriction with RsaI and BmyI. The primers were highly specific for OHV-2 DNA with a limit of detection of 6.4 pg of genomic DNA derived from the parent LCL. This was estimated to correspond to one diploid bovine cell. The PCR was successfully applied to detect OHV-2 DNA in peripheral blood leucocytes (pbl) from clinical cases of SA-MCF and normal sheep.

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“…PCR for proving AHV 1 DNA was developed by Hsu et al (1990). Baxter et al (1993) have developed specific nested PCR based on targeting a DNA fragment in the ORF 75 of OHV 2 and have confirmed the mortality in 21 bison (Bison bison) infected with SA-MCF (Berezowski et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Isolation and identification of malignant catarrhal fever virus in cell cultures

Hristov¹,

Peshev²

2016

BJVM

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Vet. Med., 19, No 4,[263][264][265][266][267][268][269][270][271][272][273] Malignant catarrhal fever (MCF) is a fatal disease syndrome responsible for mortality in domestic and wild ruminant species. MCF is caused by gammaherpesviruses -the ovine herpesvirus type 2 causing sheep-associated malignant catarrhal fever (SA-MCF) and alcelaphine herpesvirus type 1 known as wildebeest-associated MCF (WA-MCF). The present study described the cultural peculiarities of MCF virus isolated from different samples originated from dead and alive wild animals from the Sofia Zoo. MDBK, EBTR, RK, MA-104 and VERO cell cultures were used for the isolation of MCF virus. The best growth of viruses was observed on MDBK cell cultures. The CPE was characterised with forming of the syncytium and destruction of the monolayers 2-3 days after the virus adaptation. The CPE was different for obtained isolates. The isolates from gaur formed a bigger cell syncytium than that in the cell cultures infected with buffy coat from bisons where a cell syncytium of smaller size and shape was observed. The virus identification was performed by biochemical methods and PCR.

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A diagnostic method to detect alcelaphine herpesvirus-1 of malignant catarrhal fever using the polymerase chain reaction

Cited by 21 publications

References 21 publications

Detection of active and latent feline herpesvirus 1 infections using the polymerase chain reaction

Detection of active and latent feline herpesvirus 1 infections using the polymerase chain reaction

PCR detection of the sheep-associated agent of malignant catarrhal fever

Isolation and identification of malignant catarrhal fever virus in cell cultures

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