A Diet and Methods for the in vitro Rearing of the Tachinid, Eucelatoria sp.134

In vitro culture of the egg parasitoid Telenomus heliothidis: the role of teratocytes and medium consumption in development

“…However, with rare exception parasitoids have been reared on chemically undefined media which have been developed through a process of trial and error, and with a minimum knowledge of parasitoid development in its natural host (Greany, 1986;Thompson, 1986). As with the current study, even though parasitoids have been reared to adulthood, it is usually not with the success achieved with natural hosts (Boul6treau, 1972; Hoffman et al, 1974;Nettles et al, 1980;Thompson, 1981;. For example, several egg parasitoids of Lepidoptera, including T. heliothidis of the current study, have been reared on diets of similar composition (Liu et al, 1979;Ding et al, 1980;Wu et al, 1980).…”

Production of Dipteran Parasitoids

“…Nevertheless, the addition of 20-OH ecdysone to the media was necessary to trigger the first molt in P. nigrolineata and P. rufifrons (Grenier, 1988b;Fanti, 1991). Other tachinids, such as L. diatraeae and E. bryani, which develop independently of host hormones, can also develop from egg to adult in media devoid of hormones Nettles et al, 1980).…”

“…Subsequently, Fanti (1991) showed that the first molt could be obtained by supplementing an artificial medium devoid of insect components with 20-OH ecdysone. Archytas marmoratus, another species exhibiting complex behavioral and physiological interactions with the host, was reared on a medium from Nettles et al (1980) and on veal homogenate-based media, but parasitoid growth and development were poor despite the addition of lepidopterous pupal extracts (Bratti, 1994;Farneti et al, 1997). Limited results were also obtained with in vitro rearing efforts of Lydella thompsoni Herting, a larval parasitoid of O. nubilalis.…”

“…Yields reached as high as 46% of the parasitoid maggots, but only after the latter had been allowed to develop in the host for the first 18-24 h before being transferred onto the artificial media. It was estimated that an average of 2000 flies per liter of medium could be obtained (Nettles et al, 1980;. Another parasitoid of lepidopterous larvae, P. laxa, was reared from firstinstar larva to adult on the same media, with low yields, whereas yields of about 80% were obtained on a subnatural substrate based on pupal homogenate of the host H. zea (Bratti and Nettles, 1995).…”

Interspecific competition between two parasitoids of Helicoverpa zea: Eucelatoria bryani and E. rubentis

“…E. bryani and E. rubentis were reared according to methods outlined by Nettles et al (1980) and Reitz & Adler (1991). E. bryani and E. rubentis were reared according to methods outlined by Nettles et al (1980) and Reitz & Adler (1991).…”