A Diet High in Resistant Starch Modulates Microbiota Composition, SCFA Concentrations, and Gene Expression in Pig Intestine

Haenen, Daniëlle; Zhang, Jing; Silva, Carol Souza da; Bosch, G.; Meer, I.M. van der; Arkel, Jeroen van; Borne, J.J.G.C. van den; Gutiérrez, O. Pérez; Smidt, Hauke; Kemp, B.; Müller, Michael; Hooiveld, Guido

doi:10.3945/jn.112.169672

Cited by 295 publications

(289 citation statements)

References 61 publications

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“…Moreover, R. bromii was suggested to be a keystone species in resistant starch degradation, particularly type 3, which is required for the other bacteria to utilize the products from resistant starch [57]. Concordantly to the other studies [16,55,56], the Ruminococcus genus (including R. bromii ) increased in relative abundance in growing pigs that were fed with a type 3 resistant starch-containing diet [30]. An alteration in gut microbiome and a predominance of beneficial bacterial populations were observed in these pigs compared to control pigs.…”

Section: Important Dietary Components For the Gut Microbiotamentioning

confidence: 72%

“…bromii , E. rectale and Roseburia spp. populations in the gut of different animal models and humans [16,55,56]. Moreover, R. bromii was suggested to be a keystone species in resistant starch degradation, particularly type 3, which is required for the other bacteria to utilize the products from resistant starch [57].…”

Section: Important Dietary Components For the Gut Microbiotamentioning

confidence: 99%

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Modulation of the gut microbiota by prebiotic fibres and bacteriocins

Umu

Rudi

Diep

2017

Microbial Ecology in Health and Disease

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The gut microbiota is considered an organ that co-develops with the host throughout its life. The composition and metabolic activities of the gut microbiota are subject to a complex interplay between the host genetics and environmental factors, such as lifestyle, diet, stress and antimicrobials. It is evident that certain prebiotics, and antimicrobials produced by lactic acid bacteria (LAB), can shape the composition of the gut microbiota and its metabolic activities to promote host health and/or prevent diseases. In this review, we aim to give an overview of the impact of prebiotic fibres, and bacteriocins from LAB, on the gut microbiota and its activities, which affect the physiology and health of the host. These represent two different mechanisms in modulating the gut microbiota, the first involving exploitative competition by which the growth of beneficial bacteria is promoted and the latter involving interference competition by which the growth of pathogens and other unwanted bacteria is prevented. For interference competition in the gut, bacteriocins offer special advantages over traditional antibiotics, in that they can be designed to act towards specific unwanted bacteria and other pathogens, without any remarkable collateral effects on beneficial microbes sharing the same niche.

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Section: Important Dietary Components For the Gut Microbiotamentioning

confidence: 72%

Section: Important Dietary Components For the Gut Microbiotamentioning

confidence: 99%

Modulation of the gut microbiota by prebiotic fibres and bacteriocins

Umu

Rudi

Diep

2017

Microbial Ecology in Health and Disease

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“…The analysis of faecal microbiota was carried out using a phylogenetic microarray comprehensively targeting pig GI tract microbiota, the so-called Pig Intestinal Tract Chip (PITChip), developed by Pérez Gutiérrez (2010) and successfully applied by Haenen et al (2013). The updated version of the PITChip (V2.0) used in this study contained over 3300 oligonucleotides targeting 782 bacterial phylotypes reported to occur in the porcine gastrointestinal tract.…”

Section: Microbial Composition Profilingmentioning

confidence: 99%

“…Faecal microbiota composition was assessed using the Pig Intestinal Tract Chip (PITChip; Pérez Gutiérrez, 2010;Haenen et al 2013), and activity was evaluated using in vitro gas production kinetics and fermentation end-product profiles.…”

Section: Introductionmentioning

confidence: 99%

Adaptation of faecal microbiota in sows after diet changes and consequences for in vitro fermentation capacity

Sappok

Gutiérrez

Smidt

et al. 2015

Animal

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In vitro gas production studies are routinely used to assess the metabolic capacity of intestinal microbiota to ferment dietary fibre sources. The faecal inocula used during the in vitro gas production procedure are most often obtained from animals adapted to a certain diet. The present study was designed to assess whether 19 days of adaptation to a diet are sufficient for faecal inocula of pigs to reach a stable microbial composition and activity as determined by in vitro gas production. Eighteen multiparous sows were allotted to one of two treatments for three weeks: a diet high in fibre (H) or a diet low in fibre (L). After this 3-week period, the H group was transferred to the low fibre diet (HL-treatment) while the L group was transferred to the diet high in fibre (LH-treatment). Faecal samples were collected from each sow at 1, 4, 7, 10, 13, 16 and 19 days after the diet change and prepared as inoculum used for incubation with three contrasting fermentable substrates: oligofructose, soya pectin and cellulose. In addition, inocula were characterised using a phylogenetic microarray targeting the pig gastrointestinal tract microbiota. Time after diet change had an effect ( P < 0.05) on total gas production for the medium-fast fermentable substrates; soya pectin and oligofructose. For the more slowly fermentable cellulose, all measured fermentation parameters were consistently higher ( P < 0.05) for animals in the HL-treatment. Diet changes led to significant changes in relative abundance of specific bacteria, especially for members of the Bacteroidetes and Bacilli, which, respectively, increased or decreased for the LH-treatment, while changes were opposite for the HL-treatment. Changing the diet of sows led to changes in fermentation activity of the faecal microbiota and in composition of the microbiota over time. Adaptation of the microbiota as assessed by gas production occurred faster for LH-animals for fast fermentable substrates compared with HL-animals. Overall, adaptation of the large intestinal microbiota of sows as a result of ingestion of low and high fibre diets seems to take longer than 19 days, especially for the ability to ferment slowly fermentable substrates.

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“…In general, pigs are unable to produce endogenous enzyme for Resistant Search (RS) and thus seek helps from intestinal microbiota which convert it to short chain fatty acid. Haenen et al (2013) stated that diet with high RS modulate the microbial composition in colon and caecal which stimulate the expression of monocarboxylate transporter 1 (SLC16A1) and glucagon (GCG). Feeding strategy is one of the most important factor that control the quality of meat, safety and nutritional value.…”

Section: Application Of Nutrigenomics In Animal Sectormentioning

confidence: 99%

Applications of Nutrigenomics in Animal Sectors: A Review

Banerjee¹,

Pal²,

Ray³

2015

Asian J. of Animal and Veterinary Advances

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Nutrigenomics is the study of gene expression or metabolic pathway depending on different food material. In recent year, nutrigenomics has gained special attention due to its great potentiality for treating chronic disease. In this review, we have emphasized the importance of nutrigenomics on human health and on animal farm, specially on pork and poultry industries. Types of food consumption are crucial factor for human health in term of specific nutrition related gene expression and this knowledge is important for preparation of personalized diet. Human genome project identified several polymorphic gene and few of them are involved in metabolic pathway. The present review demonstrated the metabolism related disease like lactose intolerance and phenylketoneuria which is occurred due to polymorphic nature of that particular gene. Due to high cost of raw material, feed efficiency in pork and poultry industries is a crucial factor. In several investigations, researchers have used different combination of food material to monitor the growth and health of pig and chicken but it needs more accuracy and better understanding. Though the food product development and field application technique has a long way to go, further development in the field of nutrigenomics will definitely show it's potentiality in the food market and the growth of individuals. A greater understanding of these mechanisms will lead us to a sustainable fisheries, more production and other aquaculture activities.

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A Diet High in Resistant Starch Modulates Microbiota Composition, SCFA Concentrations, and Gene Expression in Pig Intestine

Cited by 295 publications

References 61 publications

Modulation of the gut microbiota by prebiotic fibres and bacteriocins

Modulation of the gut microbiota by prebiotic fibres and bacteriocins

Adaptation of faecal microbiota in sows after diet changes and consequences for in vitro fermentation capacity

Applications of Nutrigenomics in Animal Sectors: A Review

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