A diet supplemented with thiolic anti‐oxidants improves leucocyte function in two strains of prematurely ageing mice

Guayerbas, Noelia; Puerto, Marta; Ferrandez,; Fuente, Mónica De la

doi:10.1046/j.1440-1681.2002.03758.x

Cited by 28 publications

(20 citation statements)

References 48 publications

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“…1% (w/w)) by adult female Swiss and BALB/c mice for a short period of time (4-5 weeks) improves several immune functions such as chemotaxis, lymphoproliferative response to the mitogen concanavalin A, IL-2 release and NK activity in leucocytes from peritoneum, axillary nodes, spleen and thymus (39,49) .…”

Section: Effects Of a Diet Supplemented With Antioxidants On A Model mentioning

confidence: 99%

Murine models of premature ageing for the study of diet-induced immune changes: improvement of leucocyte functions in two strains of old prematurely ageing mice by dietary supplementation with sulphur-containing antioxidants

Fuente

2010

Proc. Nutr. Soc.

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Several immune functions are markers of health, biological age and predictors of longevity. A chronic oxidative and inflammatory state is the main cause of ageing and the immune system is involved in the rate of ageing. Thus, several murine models of premature ageing have been proposed owing to their early immunosenescence and oxidative stress, such as ovariectomised rats and mice, obese rats and anxious mice. In the last model, the most extensively studied by us, mice showing anxiety have an aged immune function and redox status as well as a shorter longevity in comparison with animals without anxiety of the same chronological age, being denominated prematurely ageing mice. A confirmation of the above is that the administration of diets supplemented with antioxidants improves the redox status and immune functions and increases the longevity of prematurely ageing mice. Antioxidant precursors of glutathione such as thioproline or N-acetylcysteine, which have a relevant role in ageing, have been the most widely investigated in adult prematurely ageing mice in our laboratory. In the present work, we have studied the effects of the ingestion for 5 weeks of a diet supplemented with 0 . 1 % (w/w) thioproline + N-acetylcysteine on several functions of leucocytes from chronological old (69-73 weeks of age) prematurely ageing mice of two strains (Swiss and BALB/c). The results show an improvement of the immune functions, with their values becoming closer to those in adult animals (24°2 weeks). Thus, an adequate nutrition with antioxidants, even in aged subjects, could be a good strategy to retard ageing.

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Section: Effects Of a Diet Supplemented With Antioxidants On A Model mentioning

confidence: 99%

Murine models of premature ageing for the study of diet-induced immune changes: improvement of leucocyte functions in two strains of old prematurely ageing mice by dietary supplementation with sulphur-containing antioxidants

Fuente

2010

Proc. Nutr. Soc.

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“…NAC has also been used to counteract the effects of ROS that are thought to contribute to immunological senescence. Specifically, NAC administration in a murine model of premature aging [6] and in elderly women [7] has been reported to enhance lymphocyte chemotaxis and proliferation, but suppress lymphocyte adherence ex vivo . The effects of NAC treatment on lymphocyte development were not reported in these experiments.…”

Section: Introductionmentioning

confidence: 99%

N-Acetylcysteine Increases the Frequency of Bone Marrow Pro-B/Pre-B Cells, but Does Not Reverse Cigarette Smoking-Induced Loss of This Subset

et al. 2011

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BackgroundWe previously showed that mice exposed to cigarette smoke for three weeks exhibit loss of bone marrow B cells at the Pro-B-to-pre-B cell transition, but the reason for this is unclear. The antioxidant N-acetylcysteine (NAC), a glutathione precursor, has been used as a chemopreventive agent to reduce adverse effects of cigarette smoke exposure on lung function. Here we determined whether smoke exposure impairs B cell development by inducing cell cycle arrest or apoptosis, and whether NAC treatment prevents smoking-induced loss of developing B cells.Methodology/Principal FindingsGroups of normal mice were either exposed to filtered room air or cigarette smoke with or without concomitant NAC treatment for 5 days/week for three weeks. Bone marrow B cell developmental subsets were enumerated, and sorted pro-B (B220+CD43+) and pre-B (B220+CD43−) cell fractions were analyzed for cell cycle status and the percentage of apoptotic cells. We find that, compared to sham controls, smoke-exposed mice have ∼60% fewer pro-B/pre-B cells, regardless of NAC treatment. Interestingly, NAC-treated mice show a 21–38% increase in total bone marrow cellularity and lymphocyte frequency and about a 2-fold increase in the pro-B/pre-B cell subset, compared to sham-treated controls. No significant smoking- or NAC-dependent differences were detected in frequency of apoptotic cells or the percentage cells in the G1, S, or G2 phases of the cycle.Conclusions/SignificanceThe failure of NAC treatment to prevent smoking-induced loss of bone marrow pre-B cells suggests that oxidative stress is not directly responsible for this loss. The unexpected expansion of the pro-B/pre-B cell subset in response to NAC treatment suggests oxidative stress normally contributes to cell loss at this developmental stage, and also reveals a potential side effect of therapeutic administration of NAC to prevent smoking-induced loss of lung function.

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“…With respect to the adherence and chemotaxis of lymphocytes -two functions that these cells share with phagocytes -the changes with ageing were similar to those in macrophages. In previous studies we have also observed an age-related increase of adherence and a decrease of chemotaxis in lymphocytes from old mice, adult prematurely ageing mice and old men and women (Guayerbas et al 2002b, Viveros et al 2007, Arranz et al 2008, De la Fuente and Miquel 2009. Similar changes have been shown in peritoneal lymphocytes from mice with endotoxic shock, a model of an acute oxidative stress situation (De la Fuente and .…”

Section: Phagocytic Efficiencymentioning

confidence: 58%

“…In addition, in postmenopausal women NAC showed similar effects (Arranz et al 2008). The ingestion of a diet supplemented with NAC and other thiolic precursors namely thioproline, also improved many leucocyte functions in adult, prematurely ageing mice and old animals (Blanco et al 1999, De la Fuente et al 2002, Guayerbas et al 2002b, 2004, De la Fuente 2010. NAC in vitro stimulated several functions of lymphocytes and macrophages from adult mice (Del Rio et al 1998, Pomaki et al 2005, De la Fuente et al 2011b and human subjects (Karlsson et al 2011).…”

Section: Introductionmentioning

confidence: 95%

The antioxidant N-acetylcysteine in vitro improves several functions of peritoneal leucocytes from old mice approaching their values to those of adult animals

Fuente

Hernánz

2012

J Appl Biomed

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The age-related deterioration of the function of immune cells, or immunosenescence, is based on oxidative stress (an imbalance between the levels of oxidants and antioxidant defences with an increase of the former). Accordingly, the ingestion of a diet supplemented with thiolic antioxidants such as N-acetylcysteine (NAC), a glutathione precursor, by aged subjects improved their leucocyte functions. The aim of the present study was to show if NAC improves in vitro several functions of leucocytes from chronologically old mice and if this antioxidant is able to bring the values of these functions to the levels of those of adult animals. Six concentrations of NAC (in a range from 0.001 mM to 2.5 mM) were investigated on several functions of peritoneal leucocytes from old (78±2 weeks of age) BALB/c mice. These functions were those of the phagocytic process in macrophages, namely: adherence to substrate, directed migration or chemotaxis, phagocytosis of inert particles and superoxide anion levels as a measure of digestion capacity, as well as of the adherence and chemotaxis of lymphocytes. These functions were also studied in peritoneal leucocytes from adult (18±2 weeks of age) mice. The results showed that NAC in vitro improves all the functions studied, especially at the highest concentrations, which had shown impaired values in old mice, approaching those of adult animals. Since the immune functions studied are markers of health and predictors of longevity, the administration to aged subjects of NAC, which shows a direct action in leucocytes, seems to be a good strategy to improve their immune system and, therefore, to reach a healthy longevity.

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A diet supplemented with thiolic anti‐oxidants improves leucocyte function in two strains of prematurely ageing mice

Cited by 28 publications

References 48 publications

Murine models of premature ageing for the study of diet-induced immune changes: improvement of leucocyte functions in two strains of old prematurely ageing mice by dietary supplementation with sulphur-containing antioxidants

Murine models of premature ageing for the study of diet-induced immune changes: improvement of leucocyte functions in two strains of old prematurely ageing mice by dietary supplementation with sulphur-containing antioxidants

N-Acetylcysteine Increases the Frequency of Bone Marrow Pro-B/Pre-B Cells, but Does Not Reverse Cigarette Smoking-Induced Loss of This Subset

The antioxidant N-acetylcysteine in vitro improves several functions of peritoneal leucocytes from old mice approaching their values to those of adult animals

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