A differential indicator method of identifying zones of ischemia and necrosis in rats with experimental myocardial infarction

Сернов, Л. Н.; Gatsura, V. V.

doi:10.1007/bf00841760

Cited by 7 publications

(5 citation statements)

References 6 publications

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“…To determine the size of heart ischemia and necrosis, the differential indicator method by Sernov and Gatzura (1989) was used, in our modification allowing reperfusion of the myocardium 120 minutes after an acute ischemia onset. The intensity of lipid peroxidation processes and the antiradical potency of myocardial tissue was studied by chemiluminescent method using Emilit EL photometer (USA).…”

Section: Experimental Methodsmentioning

confidence: 99%

2-aminoaethanesulfonic acid compounds possess protective property in reperfusion-induced heart jnjury

Kurganov¹,

Blinova²,

Семелева³

et al. 2018

RRP

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The study aim was to explore pharmacological effects of 2-aminoaethansulfonic acid compounds in reperfusion-induced heart injury. Materials and methods.The study was performed on rats and dogs of both sexes, isolated rats' hearts. Two compounds of 2-aminoethanesulfonic acid, magnesium-containing (LBK-527) and phenylacetamide-containing (LKhT-317) were investigated. Antiarrhythmic effects of the compounds were studied in coronary artery reperfusion 7, 30 and 120 min after acute myocardial ischemia modeling. The ability of the substances to limit the volume of reperfusion injury was investigated by differential indicator method. The influence of substances on the intensity of free radical processes in the myocardium, as well as the metabolic profile of coronary venous blood during reperfusion, was studied. Hemodynamic effects of the substances were studied during in vivo experiments, as well as on an isolated heart.Results and discussion. The compounds effectively prevent cardiac arrhythmias generation caused by myocardial reperfusion after 7, 30 and 120 minutes of ischemia. Prophylactic intravenous administration of LHT-317 and LBK-527 at higher therapeutic doses limit the size of rats' heart necrosis zone after occlusion-reperfusion syndrome develops, prevent reperfusion-induced excessive activation of free-radical processes in rat myocardium, activate the antiradical activity of the heart tissues, and optimize [O 2 ] and [CO 2 ] in coronary venous sinus blood of dogs. Cardioprotective effect of the compounds manifests in preserving myocardium contractile function, maintaing BP and stabilizing heart chronotropic function.Conclusions. The study analysis shows that 2-aminoethanesulfonic acid compounds have cardioprotective effect in reperfusion syndrome. Keywordsischemic and reperfusion heart injury, 2-aminoaethanesulfonic acid compounds, arrhythmia, hemodynamics, lipid oxidation, metabolic profile, isolated heart.

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Section: Experimental Methodsmentioning

confidence: 99%

2-aminoaethanesulfonic acid compounds possess protective property in reperfusion-induced heart jnjury

Kurganov¹,

Blinova²,

Семелева³

et al. 2018

RRP

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“…MI was provoked by ligating the descendant branch of the left coronary artery immediately below the auricula [7].…”

Section: Methodsmentioning

confidence: 99%

Comparative study of cardioprotective and antiradical activity of estrogens and their nitro derivatives

et al. 1999

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Cardioprotective and antiradical activities of estrogens and their nitro derivatives are compared. Antiradical activity was observed in estradiol, ethanol estradiol, and estradiol nitrate, but not in nystranol, which exhibited antiradical properties only after acid hydrolysis. The data obtained on hearts from rats with experimental myocardial infarction show that estrogens and their nitro derivatives restrict the area of myocardial infarction due to antiischemic and/or antinecrotic activities. Key Words: antiradical activity; nitro estrogens; experimental myocardial infarctionConsiderable attention is now focused on clinical application of estrogens and their synthetic analogs for preventing and treating cardiovascular diseases not only in women, but also in men [2,6].There is evidence that natural estrogen 17~-estradiol possesses a cardioprotective effect and restricts the area of myocardial infarction (MI) [5].In this connection it is of interest to study modified synthetic estrogens carrying an ONO 2 group, potential donor of nitric oxide. These derivatives are nystranol and estradiol nitrate.Chemical structure of nystranol and estradiol nitrate suggests that they could affect the cardiovascular system more efficiently than classical estrogens due to generation of bioactive NO group.Natural estrogens have a pronounced antiradical activity [3]. There are no such data on the nitro estrogens. Bearing in mind the important role of lipid peroxidation in the pathogenesis of MI, examination of estrogen nitro derivatives for potential antiradical activity would have a practical resonance.Our aim was to compare cardioprotective and antiradical activity of estrogens and their nitro derivatives MATERIALS AND METHODSExperiments were carried out on outbred albino rats of both sexes weighing 200-300 g. Estradiol (17~-estradiol), ethanol estradiol, nystranol (9t~-oxy-1 l~-nitroxyethynylestradiol diacetate), and estradiol nitrate (17 estradiol nitrate) were injected intraperitoneally in a dose of 10 mg/kg (in 20% ethanol) one hour before coronary occlusion. MI was provoked by ligating the descendant branch of the left coronary artery immediately below the auricula [7].The effect of steroids on the size of experimental MI was assessed after 4-h coronary occlusion.Estrogens and their nitro derivatives were tested for antiradical activity in the reaction of azo-bis-isobutyronitrile-induced oxidation of isopropylbenzene (cumol) as described elsewhere [9,10]. Test substance was added to cumol in a concentration of 5• -3 M. Nystranol was hydrolyzed with 0.1 M HCI. Oxidation kinetics was assessed by oxygen consumption measured using a gasometric setup. To determine the rate constant of interaction of the inhibitor with cumol peroxide radicals (K7), the kinetic curve of oxygen absorption was plotted in the semilogarithmic scale convert-0007-4888/99/0010-1009522.00 9Kluwer Academic/Plenum Publishers

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“…The most integral parameter, the size of the necrotic zone in acute myocardial infarction, served as the measure of the antiischemic properties of cytochrome C. To this end, myocardial infarction was modeled in artificially ventilated Laboratory of Antiischemic Preparations, Russian Research Center for the Safety of Bioactive Compounds, Staraya Kupavna, Moscow Region animals by ligation of the descending branch of the left coronary artery at the level of the bottom margin of the auricle. The rats were sacrificed 4 hours after coronary occlusion and the size of the necrotic and ischemic zones was determined by the differential indicator method [3], based on differential quantitative assay of Evans blue (ischemic zone) and formazan red (necrosis).…”

Section: Methodsmentioning

confidence: 99%

Antiischemic activity of immobilized cytochrome C

Сернов¹,

Zyuzina²

1996

Bull Exp Biol Med

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A differential indicator method of identifying zones of ischemia and necrosis in rats with experimental myocardial infarction

Cited by 7 publications

References 6 publications

2-aminoaethanesulfonic acid compounds possess protective property in reperfusion-induced heart jnjury

2-aminoaethanesulfonic acid compounds possess protective property in reperfusion-induced heart jnjury

Comparative study of cardioprotective and antiradical activity of estrogens and their nitro derivatives

Antiischemic activity of immobilized cytochrome C

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